Remediation of hepatic fibrosis as a result of the use of CCl4 and Ethanol by Chitosan Nano-Green Tea Extract: Quantification and Ultrastructural Studies

A Quantitative Description of Lipid and Extracellular Matrix Proteinaceous Fibers in Hepatic Fibrosis of a Rat Model by ImageJ using Nano-Images

“…This paper aims at confirming once again what have been published earlier [5] about lipid accumulation and ECM fiber formation during the process of hepatic fibrosis by adopting combined ImageJ and Origin Pro 2017 for statistical Analysis [5][6][7]. These two Freeware combined for the first time -in analyzing nanoscopy images obtained from fibrotic group and group treated with nanogreen tea extract providing this new tool for exploring and re-enforcing the presence of the two district features that occurred during the various stages of hepatocirrhosis; lipid bodies and extracellular matrix fibers and how nanogreen tea extract remediates both [4].…”

“…Comparing to the one treated with nanogreen tea extract in the presence of CCl 4 and ethanol 22% and the zero in control. The total damaged are in control was found as 15.9% and in CCl 4 /ethanol group it was 44.7% and in CCl 4 / ethanol/Nano GTE was 21% [5,9,12,15].…”

“…Comparing to the one treated with nanogreen tea extract in the presence of CCl 4 and ethanol 20% and with reference to the control group of zero damage. The total damaged are in control was found as 100749 and mean 153.00 while in CCl 4 /ethanol group it was 314540 and mean 114.00 in CCl 4 /ethanol/ CCl4/ethanol/NanoGTE was 3145728 with mean 129.00 [5].…”

“…Quantifications for the damaged, undamaged, and complete area of the tissue were carried out in pixels and the results were expressed in percentage of the ratio of the damaged to the complete tissue area [5]. To quantify the artificially made red-stained collagen in an image of a rat liver tissue section stained with H/E and/or Masson's Trichrome stains Table 2.…”

“…In several of our recently published works, although we have managed to establish their presence as an outcome of liver fibrosis and how nanogreen tea extract managed to remediate it in several technics [4,5] yet the analysis of their features in tissues often remains qualitative. Thus, quantitative microscopy technique for imaging localized lipid and ECM fibers environments and measuring them in fixed tissues is needed to be explored.…”