Remineralization of early enamel lesions using casein phosphopeptide amorphous calcium Phosphate: An ex-vivo study

Vashisht, Ruchi; Kumar, Anil; Indira, R; Srinivasan, Manali Ramakrishnan; Ramachandran, S

doi:10.4103/0976-237x.76385

Cited by 28 publications

(4 citation statements)

References 9 publications

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“…However, there was no significant difference between the 1450 ppm NaF toothpaste-positive control (Group 4). Similar to our results, Vashisht et al (46) they were reported that CPP-ACP indicated significant remineralization against the control group (deionized water). CPP-ACP promotes the stabilization of calcium and phosphate ions and mineral deposition in an acidic environment for enhanced buffering.…”

Section: Discussionsupporting

confidence: 92%

Effect of Different Remineralization Agents on Artificial Caries Lesion: An in-vitro Study

AKBEYAZ ŞİVET,

PARLAKYILDIZ GÖKÇE,

KARGUL

2023

Clinical and Experimental Health Sciences

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Objective: Remineralization technologies have been shown to arrest or reverse early carious lesions. This study aimed to evaluate and compare the effect of different agents on enamel remineralization in-vitro. Methods: Thirty-five enamel blocks were prepared and divided into the following groups: Group 1; CaGp and Xylitol-containing gel (R.O.C.S Medical Mineral Gel), Group 2; 1100 ppm NaF and 10% CPP-ACP toothpaste (MI Paste One), Group 3; 0.45% SnF2-1150 ppm F toothpaste (Enamelon), Group 4; Positive Control-1450 ppm NaF toothpaste (Colgate Total) and Group 5; Negative Control (deionized water). Microhardness was measured at baseline, after demineralization, and after respective treatments for different treatment groups using a digital Micro Vickers Hardness Tester. For producing demineralized lesions, samples were stored in acidic hydroxyethylcellulose (HEC, pH=4.8) for three days. The Paired Sample t-test, one-way ANOVA, and Tukey were used to compare data and SMH recovery (%SMHR) calculated among treatments. Results: The mean baseline surface microhardness value was statistically non-significant between the groups (p=.378). CaGP and Xylitolcontaining gel demonstrated having the most protective effect against demineralization. The surface remineralization potential of 1100 ppm NaF and 10% CPP-ACP containing novel toothpaste (MI Paste One) was almost similar to the positive control (1450 ppm Fluoride toothpaste)group (p>.05). Conclusions: All treatment groups showed remineralization after respective treatments and these agents can be used as an effective preventive measure for pediatric patients.

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Section: Discussionsupporting

confidence: 92%

Effect of Different Remineralization Agents on Artificial Caries Lesion: An in-vitro Study

AKBEYAZ ŞİVET,

PARLAKYILDIZ GÖKÇE,

KARGUL

2023

Clinical and Experimental Health Sciences

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“…The pH cycling protocol adopted for this study was based on the model described by Featherstone et al [ 45 ]. Various studies have performed the pH cycling process at different lengths of time ranging from 7 to 14 days [ 46 , 47 , 48 ]. In this study, a 28-day pH cycling protocol was chosen because it is believed that this was a long enough period to evaluate the potential efficacy of the RA.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Remineralizing Potential of Biomimetic Materials on Early Artificial Caries Lesions after 28 Days: An In Vitro Study

et al. 2023

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This study aimed to evaluate the loss of mineral content in the enamel surface in early artificial lesions and to assess the remineralizing potential of different agents by means of SEM coupled with energy-dispersive X-ray analysis (EDX). The analysis was performed on the enamel of 36 molars divided into six equal groups, in which the experimental ones (3–6) were treated using remineralizing agents for a 28-day pH cycling protocol as follows: Group 1, sound enamel; Group 2, artificially demineralized enamel; Group 3, CPP-ACP treatment; Group 4, Zn-hydroxyapatite treatment; Group 5, NaF 5% treatment; and Group 6, F-ACP treatment. Surface morphologies and alterations in Ca/P ratio were evaluated using SEM-EDX and data underwent statistical analysis (p < 0.05). Compared with the sound enamel of Group 1, the SEM images of Group 2 clearly showed loss of integrity, minerals, and interprismatic substances. Groups 3–6 showed a structural reorganization of enamel prisms, interestingly comprising almost the entire enamel surface. Group 2 revealed highly significant differences of Ca/P ratios compared with other groups, while Groups 3–6 showed no differences with Group 1. In conclusion, all tested materials demonstrated a biomimetic ability in remineralizing lesions after 28 days of treatment.

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“…Natural resources such as fluoride, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) derived from milk, and hydroxyapatite have been explored for their ability to control white spot lesions by promoting enamel remineralization [13]. These substances have shown promising results in clinical studies, demonstrating their potential as natural remedies for managing the early stages of dental demineralization.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Biological Response Elicited by Two Novel Tooth Cream Formulations of Cocos nucifera- Cell Line Studies and MTT Assay on Human Gingival Fibroblast

Balakrishnan,

Kumar Subramanian

2024

TIJPH

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The current research endeavors to assess the biological reactions induced by two newly formulated tooth creams from Cocos nucifera. Freshly harvested coconut from the Tirupur district of Tamil Nadu underwent processing to create two distinct formulations: one utilizing coconut milk and the other lyophilized coconut extract. Tooth cream samples were prepared and tested against commercial tooth cream following ISO 10993-5 recommendations. Human gingival fibroblast cells were isolated and cultured according to approved protocols, and cytotoxicity evaluations were conducted through MTT assay and live/dead staining. Results indicated high cell viability in both coconut-based formulations, comparable to the commercial tooth cream. Live/dead staining revealed predominantly live cells with minimal cytotoxic effects. Novel coconut-derived tooth creams exhibit comparable biocompatibility to commercial formulations, showing high cell viability and minimal cytotoxicity with human gingival fibroblasts. This suggests coconut-based tooth creams as safe alternatives for oral care, advocating further investigation into their efficacy and tissue safety. Overall, our findings endorse natural compound utilization in dental care formulations.

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Remineralization of early enamel lesions using casein phosphopeptide amorphous calcium Phosphate: An ex-vivo study

Cited by 28 publications

References 9 publications

Effect of Different Remineralization Agents on Artificial Caries Lesion: An in-vitro Study

Effect of Different Remineralization Agents on Artificial Caries Lesion: An in-vitro Study

Assessment of the Remineralizing Potential of Biomimetic Materials on Early Artificial Caries Lesions after 28 Days: An In Vitro Study

Evaluation of Biological Response Elicited by Two Novel Tooth Cream Formulations of Cocos nucifera- Cell Line Studies and MTT Assay on Human Gingival Fibroblast

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