Remineralization of teeth with casein phosphopeptide-amorphous calcium phosphate: analysis of salivary pH and the rate of salivary flow

Abstract

Aim To investigate the changes of salivary flow rate and salivary pH of Syrian children with mixed dentition following application of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP). Methods This study is part of a double-blind randomized controlled clinical trial. It included 50 children aged 6–8 who were randomly divided into two treatment groups to receive either CPP-ACP GC Tooth Mousse™ (Group A) or placebo (Group B) with 25 participants per group. After the application of the product in the mouth for 3 min, saliva samples were collected four times (T0, T1, T2, and T3) to measure salivary pH and the rate of salivary flow. Results There was no significant difference between group A and B in the mean value of salivary flow rate (t = 1.08, P = 0.28, 0.57 ± 0.28 versus 0.56 ± 0.38 respectively) and salivary pH (t = 0.61, P = 0.54, 7.28 ± 0.44 versus 7.25 ± 0.36 respectively). However, there was a significant difference between different time points (T0, T1, T2, and T3) in the mean value of salivary flow rate (0.41 ± 0.30, 0.65 ± 0.36, 0.53 ± 0.28, and 0.56 ± 0.34 respectively) and salivary pH (6.99 ± 0.44, 7.46 ± 0.36, 7.36 ± 0.32, and 7.26 ± 0.32 respectively). Conclusion The application of the GC Tooth Mouse (CPP-ACP) was similar to placebo in increasing the salivary pH and salivary flow rate. Trial registration ISRCTN17509082, Registration date 22/11/2022.

Preventive efficacy of 38% silver diamine fluoride and CPP-ACP fluoride varnish on molars affected by molar incisor hypomineralization in children: A randomized controlled trial

“…Furthermore, a recent study showed that a single topical application of the GC Tooth mousse (CPP-ACP) does not affect increasing the salivary pH and salivary flow rate which are great factors in caries prevention. 36 …”

“…35 Furthermore, a recent study showed that a single topical application of the GC Tooth mousse (CPP-ACP) does not affect increasing the salivary pH and salivary flow rate which are great factors in caries prevention. 36 On the other hand, both SDF and CPP-ACPFV showed comparable performance in preventing enamel breakdown (PEB). This indicates that both materials were effective in preserving the integrity of the enamel surface and preventing further deterioration of the affected teeth.…”

Effect of various types of milk on salivary pH among children: a pilot randomized controlled crossover trial

“…[ 27 , 28 ] In addition, salivary pH is used to evaluate the cariogenic potential of certain foods and beverages [ 29 ]. In the current study, litmus paper was used for measuring salivary pH due to its high reliability and quick documentation [ 30 ], which is to Alkarad et al [ 25 ] study. In this study, the milk intake was 250 mL, which is equivalent to one carton of milk [ 31 ].…”

“…The salivary pH was measured after 5 (t 1 ), 10 (t 2 ), 15 (t 3 ), 30 (t 4 ), and 60 (t 5 ) minutes of milk consumption. Salivary pH was measured using a pH saliva indicator strip (Duotest pH 3.5–6.8, Macherey-Nagel® GmbH & Co. KG, Düren, Germany), which is to Alkarad et al [ 25 ] study. Children were instructed to allow saliva to flow passively in the mouth for 5 min at the six-time points then they were asked to split 2 mL of saliva into a graduated tube.…”

“…Children were instructed to allow saliva to flow passively in the mouth for 5 min at the six-time points then they were asked to split 2 mL of saliva into a graduated tube. The pH saliva indicator strip was immersed in saliva for 1 min then the color change was recorded and evaluated according to the reference color chart [ 25 ]. Both the data assessor and the statistician were blinded to group allocations.…”