Remineralization potential of bioactive glass and casein phosphopeptide-amorphous calcium phosphate on initial carious lesion: An in-vitro pH-cycling study

Abstract

Aims:The aim of this study was to evaluate and compare the remineralization potential of bioactive-Glass (BAG) (Novamin®/Calcium-sodium-phosphosilicate) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing dentifrice.Materials and Methods:A total of 30 sound human premolars were decoronated, coated with nail varnish except for a 4 mm × 4 mm window on the buccal surface of crown and were randomly divided in two groups (n = 15). Group A — BAG dentifrice and Group B — CPP-ACP dentifrice. The baseline surface microhardness (SMH) was measured for all the specimens using the vickers microhardness testing machine. Artificial enamel carious lesions were created by inserting the specimens in de-mineralizing solution for 96 h. SMH of demineralized specimens was evaluated. 10 days of pH-cycling regimen was carried out. SMH of remineralized specimens was evaluated.Statistical Analysis:Data was analyzed using ANOVA and multiple comparisons within groups was done using Bonferroni method (post-hoc tests) to detect significant differences at P < 0.05 levels.Results:Group A showed significantly higher values (P < 0.05) when compared with the hardness values of Group B.Conclusions:Within the limits; the present study concluded that; both BAG and CPP-ACP are effective in remineralizing early enamel caries. Application of BAG more effectively remineralized the carious lesion when compared with CPP-ACP.

Leucine‐rich amelogenin peptide (LRAP) as a surface primer for biomimetic remineralization of superficial enamel defects: An in vitro study

“…Some previous studies have shown that the treatment of demineralized enamel by Asp-Ser-Ser (Chung and Huang, 2013;Chung and Li, 2013a,b;Yang et al, 2014) or amelogenin (Fan et al, 2011) promotes enamel remineralization. Moreover, the ability of casein phosphopeptides to improve enamel remineralization is well proven (Reynolds, '97;Mehta et al, 2014;Zhou et al, 2014). Proteins can bind to crystal faces to accelerate or inhibit crystal growth (Shiraga et al,'92).…”

Quantitative Analysis of Remineralization of Artificial Carious Lesions with Commercially Available Newer Remineralizing Agents Using SEM-EDX- In Vitro Study”

“…CPP-ACP has remineralizing effect on artificial subsurface enamel lesions and the remineralization effect increased with increase in the usage of the paste on 1st, 5th and 10th day respectively [17]. There are studies comparing the enamel remineralization potential of CPP-ACP and BAG [18] and it was clearly shown that samples of both the groups had plugs that sealed the fissures formed by demineralization and hence it was concluded that both BAG and CPP-ACP were capable of remineralizing early carious lesion and BAG had the potential to remineralize to a greater extent when compared to CPP-ACP. In another study it was considered that both the materials are effective in repair and prevention of demineralization and BAG showed better results initially, but eventually both had similar remineralizing potential [19].…”

A comparative evaluation of remineralizing ability of bioactive glass and amorphous calcium phosphate casein phosphopeptide on early enamel lesion

“…[13] The samples were then washed under deionized water, stored in artificial saliva for 10 days ( first cycle of remineralization) after which the samples were tested for microhardness and the values were recorded. Following this, ACP-CPP and BAG were applied for another 5 days and at the end of 15 days (second cycle of remineralization), the samples were subjected for microhardness testing using the same procedure as described earlier.…”