Remineralizing effect of a zinc-hydroxyapatite toothpaste on enamel erosion caused by soft drinks: Ultrastructural analysis

Colombo, Marco; Mirando, Maria; Rattalino, Davide; Beltrami, Riccardo; Chiesa, Marco; Poggio, Claudio

doi:10.4317/jced.53790

Cited by 19 publications

(22 citation statements)

References 22 publications

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“…It is well documented in literature that tooth erosion attributed to high consumption of citric acid containing soft drinks is increasingly seen as a public health concern, thus contributing to DH. 18 The potential strategy for management of DH is to effectively occlude the dentine tubules. The purpose of this study was to synthesized and evaluate the effectiveness of a ball-milled synthesized EB@TiO 2 to occlude open dentine tubules.…”

Section: Discussionmentioning

confidence: 99%

A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study

et al. 2019

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To synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; Group 4: Sensodyne. These were then agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine preand post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.

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Section: Discussionmentioning

confidence: 99%

A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study

et al. 2019

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“…Previous studies suggested that bovine and enamel models be used when examining the effectiveness of commercial toothpaste against acidic attacks. 16,17 Also considered as the sole test model was the dissolution of hydroxyapatite (HA). 18,19 It is generally well known that tooth enamel consists mostly of calcium hydroxyapatite with a molecular formula of Ca 10 (PO 4 ) 6 (OH) 2 .…”

Section: Introductionmentioning

confidence: 99%

An in vitro examination on the effectiveness of commercial toothpastes in the prevention of tooth decay, using eggshell as a substitute for human tooth material

et al. 2018

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Background: Despite improvements in oral health status, dental caries remains a public concern. This study examines the effectiveness of commercial toothpastes in the prevention of tooth decay, using eggshell powder as a substitute for human tooth material. Methods: Colgate, Aquafresh, Colgate Sensitive, Sensodyne, and Oralwise were tested. An enamel model was simulated by adding eggshell powder to beakers containing dissolved toothpastes. The contents were agitated for eight hours at 800rpm, filtered and oven dried. Scanning Electron Microscope (SEM) and Energy Dispersive Spectroscopy (EDX) were used to characterise the surface morphology and the calcium and phosphate composition of the toothpastes pre-and post-agitation with eggshell powder. Gas-displacement and pressure sensor tests evaluated the rate of reaction between the substitute "tooth enamel" and acids. results: EDX analysis confirmed the presence of calcium phosphate ions, while SEM revealed the formation of an enamel-like layer after agitation. Pressure sensor tests confirmed Colgate Sensitive as most effective in protection against acid attacks, with Oralwise least effective. conclusions: Eggshell can be used as a substitute for the human tooth in an in vitro experiment. All the tested brands of toothpaste effectively reduced the acid reaction, which would contribute to the prevention of tooth decay.

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“…In this study, the zinc level was set at 10 mmol/L based on the recommended intake (11 mg/day) and maximum permissible intake (40 mg/day) of zinc for humans. Creeth et al [30] and Colombo et al [31] also reported in their in vitro study using dentifrices and bovine enamel specimens that zinc increased demineralization resistance. The discrepancy between the present study and these previous reports could be due to differences in experimental methods.…”

Section: Discussionmentioning

confidence: 92%

Effects of Zinc, Iron, and Copper on Bovine Enamel Erosion Evaluated Using Transverse Microradiography

Yamada¹,

Watanabe²,

Hara³

et al. 2020

Health

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Objective: This study aimed to clarify the effect of trace elements on enamel erosion due to citric acid eliminating other factors that can affect enamel erosion. Method: Forty enamel specimens were obtained by embedding bovine enamel blocks into a quick cure resin. Half of the enamel surface of the specimens was covered with nail varnish. The specimens were randomly divided into control (Ct), zinc (Zn), iron (Fe), and copper (Cu) groups and immersed in 1% citric acid solution, 1% citric acid solution with 10 mmol/L zinc, 1% citric acid solution with 10 mmol/L iron, or 1% citric acid solution with 10 mmol/L copper, respectively, at 37˚C for 5 h. After immersion, the demineralized lesion depth was measured using transverse microradiography (TMR) and compared between groups using one-way analysis of variance and Tukey's test. Results: The lesion depth was significantly higher in the Zn (218.9 ± 88.5 µm) group than that in the Ct group (116.3 ± 22.1 µm) (p < 0.01). Conclusion: It was suggested that zinc has decalcification properties when consumed with citric acid, while iron and copper don't have such properties.

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Remineralizing effect of a zinc-hydroxyapatite toothpaste on enamel erosion caused by soft drinks: Ultrastructural analysis

Cited by 19 publications

References 22 publications

A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study

A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study

An in vitro examination on the effectiveness of commercial toothpastes in the prevention of tooth decay, using eggshell as a substitute for human tooth material

Effects of Zinc, Iron, and Copper on Bovine Enamel Erosion Evaluated Using Transverse Microradiography

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