Remineralizing Efficacy of Fluorohydroxyapatite Gel on Artificial Dentinal Caries Lesion

Abstract

The aim was to evaluate the remineralizing efficacy of fluorohydroxyapatite (FHA) gel on artificial dentinal caries lesion in vitro. Artificial carious lesions were made on occlusal cavities of teeth by exposing the dentin surface to a demineralizing solution. Each cavity was capped with a 3 mm thick FHA gel for 4 weeks. After the FHA gel was removed, the surface morphology and structure of the dentin were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The dentin mineral density (DMD) was measured by micro-computed tomography (Micro-CT). A layer of dense and orderly hexagonal crystal structure, with average diameter of 1 μm and thickness of 4~5 μm, could be observed on dentin surface. These crystals exhibited elemental peaks for calcium, phosphorus, carbon, and oxygen and characteristic peaks of hydroxyapatite (HA) and fluorapatite (FA) via XRD and FT-IR. The DMD of dentin surface layer significantly increased after it was capped with FHA gel (P<0.05). In the present study, the FHA gel could rapidly construct apatite on the artificial dentin caries surface and significantly increase the mineral density, which suggests that FHA gel might be a proper IPT material with remineralizing function.

Nanofluorapatite Hydrogels in the Treatment of Dentin Hypersensitivity: A Study of Physiochemical Properties and Fluoride Release

“…Fluorapatite, with the formula Ca 10 (PO 4 ) 6 F 2 , is a biocompatible material [ 23 ] and therefore has the potential to be studied as a material for use in dental practice; it also lends itself to research into reducing dentin hypersensitivity. Earlier experiments with fluorapatite indicate its remineralizing function [ 24 ], which supports the osseointegration of implants [ 25 ], increases the mechanical parameters of glass ionomer cements for tooth reconstruction [ 26 ], and suppresses pain perception [ 27 ]. Hydrogel formulations containing a combination of fluorapatite and sodium fluoride, containing a high level of 20,000 ppm fluoride ion, have not yet been analyzed.…”

Perilaku Sikat Gigi Terhadap Terjadinya Karies Gigi Di Sekolah Dasar

“…Sebuah penelitian yang dilakukan di Kabupaten Sleman, Jawa Tengah, menyatakan bahwa perilaku menyikat gigi yang salah memiliki hubungan yang erat dengan karies gigi. (Wang et al, 2015…”

Denti̇ni̇n Bi̇yomi̇meti̇k Remi̇nerali̇zasyonu

“…Jel yapısı hidrojel bir mikro çevre sağlarken agarozun da remineralizasyon odaklarının oluşması ve büyümesinde indükleyici olarak rol aldığı düşünülmektedir 11,27,44 . Wang ve arkadaşları 27 , indirekt pulpa kaplama materyali olarak FHA jel, kalsiyum hidroksit (Ca(OH) 2 ) ve mineral trioksit agregat (MTA) kullandıkları çalışmalarında lezyon yüzeyi altı mineral yoğunluğunda ve derinliğinde üç grupta da önemli bir değişikliğin olmadığını göstermişlerdir. Bu durumun ise dentin yüzeyinde oluşan apatitin, kollajen fibrilleri arasındaki boşluğa girmek için fazla büyük olması ile açıklanabileceğini bildirmişlerdir.…”

“…Yüzey kristallerle kaplandığında daha büyük kristaller bir araya toplanır ve düzgün bir yönde dururlar. Jel yapısı hidrojel bir mikro çevre sağlarken agarozun da remineralizasyon odaklarının oluşması ve büyümesinde indükleyici olarak rol aldığı düşünülmektedir 11,27,44 .…”