Remission of chronic fungal asthma in the absence of CCR8

Toll like receptor-2 modulates both innate and adaptive immune responses during chronic fungal asthma in mice

“…This modulation of the Th2 immune response was consistent with the decreased airway-remodeling present in the TLR2 -/-group, particularly at day 30 after conidia. Our previous studies have highlighted the importance of Th2-type cytokines in the airway remodeling response associated with chronic fungal asthma [16,17,23]. Clearly, the decreased Th2 immunity in TLR2 -/-mice did not prevent the increased airway hyperresponsiveness in this group at day 30.…”

“…Major features of the Th2 immune response during chronic fungal asthma are the persistence of mucus cell metaplasia and peribronchial fibrosis [16,17,23]. As shown in Figure 4A, mucus cells were prominently stained a bright magenta color with PAS at day 30 after conidia challenge.…”

“…Total RNA was isolated from homogenized mouse lungs using Trizol reagent (Invitrogen/ Life Technologies, Carlsbad, CA) as previously described in detail [17]. Purified RNA was treated with DNAse and reverse transcribed into cDNA using TAQMAN Reverse Transcription Reagents (Foster City, CA).…”

“…The presence and function of Th2-type cytokines [22][23][24] and chemokines [17,25] have major roles in the initiation and maintenance of chronic fungal asthma in mice. The present study demonstrated that whole lung levels of IL-4, IL-5, IL-10, and CCL17 were all significantly lower in the TLR2 -/-group compared with the TLR2 +/+ group at day 3 after conidia (Fig.…”

CC Chemokine Receptors and Chronic Inflammation—Therapeutic Opportunities and Pharmacological Challenges

“…In a model of chronic fungal asthma, the absence of CCR8 receptor was shown to promote the clearance of fungal material in the lungs of these animals, highlighting the receptor as a potential therapeutic target in fungal associated pulmonary conditions (Buckland et al, 2007).…”