Remodeling Microenvironment for Implant‐Associated Osteomyelitis by Dual Metal Peroxide

Abstract

Implant‐associated osteomyelitis (IAOM) is characterized by deep bone infection and progressive bone destruction, current therapy of long‐term high‐dose antibiotic treatment and radical surgical debridement often results in drug‐resistance and large bone defect. It is highly challenging to develop an antibiotic‐free, rapid bactericidal and osteogenic‐enhanced strategy for IAOM. Herein, we design an IAOM tailored anti‐bacterial and osteoinductive composite of Cu‐Sr peroxide nanoparticles (CSp NPs) encapulated in polyethylene glycol diacrylate (PEGDA) (CSp@PEGDA). The dual functional CSp NPs spontaneously displays in situ H2O2 self‐supplying and Fenton‐catalytic Cu2+ ions release, generating plenty of highly toxic hydroxyl radical (·OH) in a pH‐responsive manner for bacterial killing, while the released Sr2+ promotes the in vitro osteogenicity regarding cell proliferation, alkaline phosphatase activity, extracellular matrix calcification, and osteo‐associated genes expression. The integration of Cu2+ and Sr2+ in synthesized CSp NPs together with the coated PEGDA hydrogel ensures the stable and sustainable ions release during short‐ and long‐term periods. Benefitted from the injectablity and photo‐crosslinkability, CSp@PEGDA is able to thoroughly fill the infectious site and rapidly gelate in situ for bacterial elimination and bone regeneration, which is successfully verified through in vivo evaluation using a clinical‐simulating IAOM mouse model. These favorable abilities of CSp@PEGDA precisely meet the multiple therapeutic needs and pave a promising way for implant‐associated osteomyelitis treatment.This article is protected by copyright. All rights reserved

Self-Assembled Multilayer-Modified Needles Simulate Acupuncture and Diclofenac Sodium Delivery for Rheumatoid Arthritis

“…Results in Figure b show that compared to the model group, the levels of p-IκBα and NF-κB decreased significantly after the treatment. NF-κB is an important factor that regulates the microenvironment in the inflammation of bone . Compared to other methods, the suppression of protein expression of p-IκBα and NF-κB in the T4 group was the most significant, implying that DS-MMAN at acupoints shows synergistic anti-inflammatory potential.…”