Remodeling of Nucleoproteins during Gametogenesis, Fertilization, and Early Development

Poccia, Dominic

doi:10.1016/s0074-7696(08)61061-x

Cited by 262 publications

(131 citation statements)

References 400 publications

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“…Sperm maturation process involves the replacement of somatic cell histones with basic transition proteins and final restoration with sperm specific protamines resulting in highly compacted chromatin with hydrodynamic nucleus and the specific morphological form of the spermatozoa [47,48]. Any deviations in this maturation process of replacement of histones by protamines may result in poor chromatin packing and severe morphologically abnormal sperms, having a significant influence on fertilization rate [24,49].…”

Section: Discussionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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Purpose The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. Methods Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. Results Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. Conclusions Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

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Section: Discussionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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“…As a result, during spermatogenesis the somatic histones are gradually replaced by protamines, to form a stable nuclear structure of mature spermatozoa [Oliva 2006;Poccia 1986]. Concomitant with this event, a substantial portion of cytoplasm is phagocytized by Sertoli cells and thereby a major source of antioxidant capacity is lost which may render the sperm more prone to oxidative stress when faced with oxidants [de Lamirande and Gagnon 1995].…”

Section: Introductionmentioning

confidence: 99%

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Kiani-Esfahani¹,

Bahrami²,

Tavalaee³

et al. 2013

Systems Biology in Reproductive Medicine

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The aim of this study was to determine if there are any associations between impaired chromatin packaging and the origin of reactive oxygen species (ROS) production. Cytosolic ROS, mitochondrial ROS, DNA protamination, and apoptosis were evaluated with dichlorofluoresceindiacetate (DCFH-DA), dihydrorhodamine 123 (DHR123), chromomycin A3 (CMA3), and YO-Pro-1 (Y1)/propidium iodide (PI), respectively, by flow cytometry (FCM) in 40 infertile individuals. Percentages DCF+ and R123+ sperm were positively associated with percentage CMA3+ sperm and negatively associated with percentage apoptotic sperm. No correlation was observed between CMA3+ sperm and the percentage of apoptotic sperm. Under protamination of sperm is not associated with the origin of ROS production, but their relationship may suggest an association with general physiological dysfunction of sperm. Furthermore, under protamination does make sperm prone to apoptosis. Rather, it is likely that apoptosis is induced by ROS production. Considering that these conclusions are derived from correlative analyses, additional studies including an interventive approach are required.

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“…Linker histones may also influence the positioning of nucleosomes (Meersseman et al 1991;Chipev and Wolffe 1992). Distinct variants of linker histones are expressed during the development of several organisms, including vertebrates (Poccia 1986). In the sea urchin (Strongylocentrotus), there are distinct sperm, cleavage, and adult linker histones (Newrock et al 1977;Levy et al 1982;Knowles et al 1987;Lai and Childs 1988).…”

mentioning

confidence: 99%

Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

Bouvet

Dimitrov²,

Wolffe³

1994

Genes Dev.

233

154

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The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone HI within chromatin facilitates the activation of the oocyte 5S rRNA genes by TFIIIA. Variation in the amount of histone HI in chromatin does not significantly influence somatic 5S rRNA gene transcription. Thus, the regulated expression of histone HI during Xenopus development has a specific and dominant role in mediating the differential expression of the oocyte and somatic 5S rRNA genes. This example demonstrates that histones can exert dominant repressive effects on the transcription of a gene in vivo in spite of an abundance of transcription factors for that gene.

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Remodeling of Nucleoproteins during Gametogenesis, Fertilization, and Early Development

Cited by 262 publications

References 400 publications

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

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