Remote ablation chamber for high efficiency particle transfer in laser ablation electrospray ionization mass spectrometry

Dolatmoradi, Marjan; Fincher, Jarod A.; Korte, Andrew R.; Morris, Nicholas J.; Vertes, Akos

doi:10.1039/d0an00984a

Cited by 1 publication

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“…It limits, however, the usefulness of LAESI as an ionization technique for MSI experiments to applications with very distinct spatial distributions of metabolites. Studies of the transport of neutral particles in remote LAESI geometries, 32 such as that of Dolatmoradi et al, 33 are therefore important for the ongoing development of the LAESI method. The transmission geometry of the optical system applied in that study, however, is unsuitable for applications on samples with a thickness beyond that of a thin section and with a three-dimensional surface topography, such as stems and whole leaves.…”

Section: Resultsmentioning

confidence: 99%

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

Bartels

Svatoš

2022

J. Am. Soc. Mass Spectrom.

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Spatially resolving the relative distribution of analyte molecules in biological matter holds great promise in the life sciences. Mass spectrometry imaging (MSI) is a technique that can provide such spatial resolution but remains underused in fields such as chemical ecology, as traditional MSI sample preparation is often chemically or morphologically invasive. Laser ablation electrospray ionization (LAESI)-MSI is a variation of MSI particularly well-suited for situations where chemical sample preparation is too invasive but provides new challenges related to the repeatability of measurement outcomes. We assess the repeatability of LAESI-MSI by sampling a droplet of [ring- 13 C 6 ] l -phenylalanine with known concentration and expressing the resulting variability as a coefficient of variation, c v . In doing so, we entirely eliminate variability caused by surface morphology or underlying true sample gradients. We determine the limit of detection (LOD) for 13 C 6 -Phe by sampling from droplets with successively decreasing but known concentration. We assess the influence of source geometry on the LOD and repeatability by performing these experiments using four distinct variations of sources: one commercial and three custom-built ones. Finally, we extend our study to leaf and stem samples Arabidopsis thaliana and Gossypium hirsutum . We overcome the challenges of LAESI associated with three-dimensional surface morphology by relying on work previously published. Our measurements on both controlled standard and realistic samples give strong evidence that LAESI-MSI’s repeatability in current implementations is insufficient for MSI in chemical ecology.

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Section: Resultsmentioning

confidence: 99%

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

Bartels

Svatoš

2022

J. Am. Soc. Mass Spectrom.

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Remote ablation chamber for high efficiency particle transfer in laser ablation electrospray ionization mass spectrometry

Abstract:
Laser ablation electrospray ionization (LAESI) driven by mid-infrared laser pulses, allows the direct analysis of biological tissues with minimal sample preparation. Dedicated remote ablation chambers have been developed to eliminate...

Cited by 1 publication

References 31 publications

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

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Remote ablation chamber for high efficiency particle transfer in laser ablation electrospray ionization mass spectrometry

Abstract: Laser ablation electrospray ionization (LAESI) driven by mid-infrared laser pulses, allows the direct analysis of biological tissues with minimal sample preparation. Dedicated remote ablation chambers have been developed to eliminate...

Cited by 1 publication

References 31 publications

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

Influence of Ion Source Geometry on the Repeatability of Topographically Guided LAESI-MSI

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Product

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About

Abstract:
Laser ablation electrospray ionization (LAESI) driven by mid-infrared laser pulses, allows the direct analysis of biological tissues with minimal sample preparation. Dedicated remote ablation chambers have been developed to eliminate...