Remote or Conventional Ischemic Preconditioning –Local Liver Metabolism in Rats Studied with Microdialysis

Björnsson, Bergþór; Winbladh, Anders; Bojmar, Linda; Trulsson, Lena; Olsson, Hans; Sundqvist, Tommy; Gullstrand, Per; Sandström, Per

doi:10.1016/j.jss.2011.07.038

Cited by 9 publications

(5 citation statements)

References 48 publications

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“…Similar results were found in a study investigating the effects of ischemia preconditioning of heart muscle prior to coronary artery occlusion [ 43 ]. In a recent study, remote ischemic preconditioning led to reduced levels of glycerol in ischemic brain tissue [ 44 ], and similar effects of direct ischemic preconditioning have been found in liver tissue [ 45 , 46 ]. Furthermore, preconditioning has been shown to sustain lactate, pyruvate and the LPR in liver, muscle, free flaps and heart [ 46 - 49 ].…”

Section: Discussionmentioning

confidence: 77%

Effects of inhalation of low-dose nitrite or carbon monoxide on post-reperfusion mitochondrial function and tissue injury in hemorrhagic shock swine

et al. 2015

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IntroductionTissue reperfusion following hemorrhagic shock may paradoxically cause tissue injury and organ dysfunction by mitochondrial free radical expression. Both nitrite and carbon monoxide (CO) may protect from this reperfusion injury by limiting mitochondrial free radial production. We explored the effects of very small doses of inhaled nitrite and CO on tissue injury in a porcine model of hemorrhagic shock.MethodsTwenty pigs (mean wt. 30.6 kg, range 27.2 to 36.4 kg) had microdialysis catheters inserted in muscle, peritoneum, and liver to measure lactate, pyruvate, glucose, glycerol, and nitrite. Nineteen of the pigs were bled at a rate of 20 ml/min to a mean arterial pressure of 30 mmHg and kept between 30 and 40 mmHg for 90 minutes and then resuscitated. One pig was instrumented but not bled (sham). Hemorrhaged animals were randomized to inhale nothing (control, n = 7), 11 mg nitrite (nitrite, n = 7) or 250 ppm CO (CO, n = 5) over 30 minutes before fluid resuscitation. Mitochondrial respiratory control ratio was measured in muscle biopsies. Repeated measures from microdialysis catheters were analyzed in a random effects mixed model.ResultsNeither nitrite nor CO had any effects on the measured hemodynamic variables. Following inhalation of nitrite, plasma, but not tissue, nitrite increased. Following reperfusion, plasma nitrite only increased in the control and CO groups. Thereafter, nitrite decreased only in the nitrite group. Inhalation of nitrite was associated with decreases in blood lactate, whereas both nitrite and CO were associated with decreases in glycerol release into peritoneal fluid. Following resuscitation, the muscular mitochondrial respiratory control ratio was reduced in the control group but preserved in the nitrite and CO groups.ConclusionsWe conclude that small doses of nebulized sodium nitrite or inhaled CO may be associated with intestinal protection during resuscitation from severe hemorrhagic shock.Electronic supplementary materialThe online version of this article (doi:10.1186/s13054-015-0903-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 77%

Effects of inhalation of low-dose nitrite or carbon monoxide on post-reperfusion mitochondrial function and tissue injury in hemorrhagic shock swine

et al. 2015

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“…This led to the monitoring of metabolic changes in liver grafts in a clinical pilot study with 10 patients (116). Metabolic products were also monitored during ischemia-reperfusion injury in the rat liver using microdialysis and commercially available bedside kits (117). This study proved that microdialysis is applicable for human liver surgery by continuous intraoperative monitoring of intrahepatic metabolism (118).…”

Section: Microdialysis Monitoringmentioning

confidence: 89%

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Priya¹,

Córcoles²

2012

Sepsis - An Ongoing and Significant Challenge

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“…This feature permits probes to be inserted virtually in any tissue with minimum disturbances to its physiology and normal parenchymal structure. Several authors have reported its utilization in subcutis Hagstrom et al, 1987;Kaptein et al, 1998;, muscle (Deboer et al, 1991;Deguchi et al, 1991;Hallstrom et al, 1989;Miller et al, 2011;Mimura et al, 1996;Olausson et al, 2013;Stallknecht et al, 1999), liver (Bjornsson et al, 2012;Lunte, 1995, 1996;Haugaa et al, 2010;Takahashi et al, 1996;Vanbelle et al, 1995), brain (Bjerring et al, 2011a, b;Bossers et al, 2013;Li et al, 2012;Notkina et al, 2012;Sato et al, 2011), adipose tissue (Arner and Bolinder, 1991;Jansson et al, 1995;Kopterides et al, 2012;Lourido et al, 2002;Song and Lunte, 1999;Summers et al, 1998), and recently in the lamellar tissue of the horse hoof (Nourian et al, 2010). Also tissue microdialysis can be used for continuous monitoring for time periods ranging from a few hours to several weeks .…”

Section: Tissue Microdialysismentioning

confidence: 99%

The effects of locally delivered insulin on equine hoof lamellar tissue

Castro¹

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I would like to thank firstly to my family for supporting me unconditionally in all my projects, in especial to my mother, who has been the main responsible of the person I am today. Secondly, I would like to express my sincere gratitude to my supervisors: Andrew van Eps, for the patience and generosity he always showed to me when it came to sharing his knowledge; Chris Pollitt, for replying that first email 3 years ago, which was the beginning of this project, for sharing his knowledge and vast experience with this young student, and for encouraging me to do more research; Martin Sillence, for trusting in my capacities and for having always the time to catch up, have a drink and give me his advice. I must thank all the people involved in this project along these 2 years: Claire Underwood, for allowing me to be part of her experiments and for all the help she gave me, especially when it was required at the very last minute; to Rajesh Gupta, for all the time and Buddhist tales shared during my training at CARF and to all the UQ and QUT staff who contributed in many different ways to this work. Finally, I want to express my sincere appreciation to all my friends, and especially to Ana and Carlos, who became my temporary family for a while and offered me a hand from the very beginning.

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Remote or Conventional Ischemic Preconditioning –Local Liver Metabolism in Rats Studied with Microdialysis

Cited by 9 publications

References 48 publications

Effects of inhalation of low-dose nitrite or carbon monoxide on post-reperfusion mitochondrial function and tissue injury in hemorrhagic shock swine

Effects of inhalation of low-dose nitrite or carbon monoxide on post-reperfusion mitochondrial function and tissue injury in hemorrhagic shock swine

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

The effects of locally delivered insulin on equine hoof lamellar tissue

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