The effect of spatial scale on spatial analysis has long, but sporadically, been recognized in could be adequately quantified using spectral information-based variables, the results and accuracy of such a analysis depended on both landscape composition and sample size. The linear response of the statistical relationship to the change in sample size over some range of scales indicated that scale effects could be readily predicted in certain cases. However, in general, because scale effects can further be complicated by the choice of variables and the idiosyncrasy of particular landscapes, the predictability of scale effects seems to be confined only to certain domains of scale. To find these domains multiple-scale or hierarchical analysis must be performed. This study further supports that the modifiable areal unit problem is a common one across the disciplinary boundaries of geography, ecology and other earth sciences.Unraveling the problem not only will improve our understanding of pattern and process in nature, but also will have important implications for appropriate use of remote sensing data and GIS.