Removable and replaceable micro-mixing system with surface acoustic wave actuators

Yamaguchi, Akinobu; Takahashi, Masatoshi; Saegusa, Shunya; Utsumi, Yuichi; Saiki, Tsunemasa

doi:10.35848/1347-4065/ad2aa4

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…The influence of chamber geometry on mixing efficiency undergoes detailed exploration in Figure S4 and Figure d. With the total chamber volume held constant at 75 μL (determined by the subsequent extraction and amplification system), , the ratio of the bottom diameter to the chamber height was varied, with mixing efficiency represented by the distinct red line in Figure d after a consistent operational period of 20 s. A critical observation arises: a small ratio, making the chamber height much greater than its diameter, limits the microvortex created by acoustic energy due to boundary constraints, thereby reducing its capacity to effectively mix the chamber’s liquid contents. This limitation is attributed to the device’s proximity to the boundary, which restricts the microvortex’s effective mixing range.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the device is more energyefficient and can be powered by portable batteries. 30,31 Amorphous Silicon Temperature Sensor and Photosensor. Figure 3a shows the cross section of the amorphous silicon chip, while Figure 3b offers a top-down view of the actual assembly.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…At this level, the liquid damping is dominant. In addition, the device is more energy-efficient and can be powered by portable batteries. , …”

Section: Resultsmentioning

confidence: 99%

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Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing

Li,

Costantini

et al. 2024

Anal. Chem.

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Lab-on-a-chip systems (LOCs), characterized by their high sensitivity, low sample consumption, and portability, have significantly advanced the field of on-site testing. Despite the evolution of integrated LOCs from qualitative to quantitative analyses, on-chip full integration of sample preparation, purification, and multiplexed detection remains a challenge. Here, we propose a strategy for the heterogeneous integration of a set of complementary metal oxide semiconductor-compatible devices including acoustic resonator, thin-film resistors, and temperature/photosensors as a new type of LOC for nucleic acid testing (NAT). Programmed acoustic streaming-based particles and fluid manipulations largely simplify the nucleic acid extraction process including cell lysis, nucleic acid capture, and elution. The design of the acoustic microextraction module and extraction process was thoroughly studied. Benefitted by the microelectromechanical system approach, the conventional mechanical actions and complex flow control are avoided, which enables a compact hand-held NAT instrument without complicated peripherals. Validation experiments conducted on plasma-harboring mutations in the epidermal growth factor receptor (EGFR) gene confirmed the robustness of the system, achieving an impressive nucleic acid (NA) extraction efficiency of approximately 90% within 5 min and a limit of detection of the target NA in the plasma of 1 copy/μL.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%