Removal by adsorbent beads of biological response modifiers released from platelets, accumulated during storage, and potentially associated with platelet transfusion reactions

Abstract

This study revealed that Cellulose Beads A or B are effective in removing the three BRMs that accumulate during PLT storage. Additional in vitro assays and in vivo studies are required to evaluate whether this method is effective in reducing transfusion reactions.

Removal of biological response modifiers associated with platelet transfusion reactions by columns containing adsorption beads

“…Therefore, a simple and convenient method is required to reduce the levels of BRMs in stored plasma PCs and washed PCs. We previously established a batch method to reduce BRMs using adsorptive cellulose beads, which adsorb the BRMs released from PLTs during storage; 24 however, the adsorption batch method requires refinement before it is of practical use in the clinical setting. Selesorb and Liposorber beads have been clinically used for many decades for immunoadsorption and LDL apheresis, respectively.…”

“…Therefore, we previously developed and evaluated in nonclinical settings an adsorption batch system, which uses adsorptive beads to remove BRMs. 24 This system was effective at reducing the levels of sCD40L, RANTES, and TGF-β1. However, the adsorption batch system is not practical for clinical uses.…”

“…Leukoreduced PCs, which have been clinically used in Japan since 2004, have reduced the incidence of febrile NHTRs but have not been able to completely eliminate it; however, there were few effects on allergic reactions. In the previous study, we showed that some BRMs, such as soluble CD40 ligand (sCD40L); regulated upon activation, normal T‐cell expressed, and secreted (RANTES; also known as CCL5); and transforming growth factor‐β1 (TGF‐β1) accumulate in proportion to the storage duration …”

“…Partial reduction in plasma levels cannot completely prevent NHTRs. Therefore, we previously developed and evaluated in nonclinical settings an adsorption batch system, which uses adsorptive beads to remove BRMs . This system was effective at reducing the levels of sCD40L, RANTES, and TGF‐β1.…”

“…In the previous study, we showed that some BRMs, such as soluble CD40 ligand (sCD40L); regulated upon activation, normal T-cell expressed, and secreted (RANTES; also known as CCL5); and transforming growth factor-β1 (TGF-β1) accumulate in proportion to the storage duration. 24 CD40 is expressed on a variety of cell types including endothelial cells, monocytes, macrophages, smooth muscle cells, and PLTs; however, the CD40 ligand (CD40L), a 39-kDa transmembrane glycoprotein, is expressed in PLTs. sCD40L is released by the cleavage of CD40L and accumulates in PCs.…”