The unrestricted discharge of domestic and industrial wastewaters along with agricultural runoff water into the environment as mixed-wastewater pose serious threat to freshwater 2 resources in many countries. Mixed-wastewater pollution is a common phenomenon in the developing countries as the technologies to treat the individual waste streams at source are lacking due to high operational and maintenance costs. Therefore, the need to explore the potential of suspended growth process (SGP) which is a well-established process technology for biological wastewater treatment, is the focus of this paper. Different wastewater constituents: representing domestic, pharmaceutical, textile, petroleum, and agricultural runoff were synthesized as a representative of mixed-wastewater and treated in two semicontinuous bioreactors (R1 & R2) operated at constant operating conditions vis., MLSS (mg/L): 4640-R1, 4440-R2, SRT: 21-d, HRT: 48 -72-h, and uncontrolled pH. The system attained stable condition in day 97, with average COD, BOD and TSS reduction as 84.5, 86.2, and 72.2% for R1; and 85.1, 87.9, and 75.1% for R2 respectively. Phosphate removal on average was by 74.3% in R1 and 76.6% in R2, while average nitrification achieved in systems 1 and 2 were 56.8and 54.7% respectively. The biological treatment system has shown potential for improving the quality of mixed-wastewater to the state where reuse may be considered and tertiary treatment can be employed to polish the effluent quality.