Removal of Acrosomes of Ram and Rabbit Spermatozoa

Srivastava, Pranay

doi:10.1530/jrf.0.0330323

Cited by 25 publications

(15 citation statements)

References 9 publications

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“…The plasma membrane and acrosomal membranes were sequentially removed using procedures that retain the activity of surface-associated and acrosomal enzymes (Srivastava, 1973;Srivastava et al, 1974). A sample of whole semen was removed by aspiration with a pastuer pipet and placed into 1 ml picric acid fixative (University of Georgia School of Veterinary Medicine EM Core Facility) in order to verify sperm membrane integrity by transmission electron microscopy (TEM) before demembranation.…”

Section: Materials and Methods Sperm Collection And Membrane Removalmentioning

confidence: 99%

Galactosyltransferase activity is restricted to the plasma membranes of equine and bovine sperm

Fayrer-Hosken

Caudle²,

Shur

1991

Molecular Reproduction Devel

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beta 1, 4-Galactosyltransferase (GalTase) is localized to the plasma membrane of mouse sperm, in which it mediates the binding of sperm to glycoconjugate residues in the egg zona pellucida. In this study, the presence of subcellular distribution of sperm GalTase were determined in two other mammalian species that yield sufficient sperm for subcellular fractionation. Equine and bovine semen were collected, and the plasma membranes (PM), outer acrosomal membranes (OAM), and inner acrosomal membranes (IAM) were sequentially removed. The purities of the isolated membrane preparations were determined by transmission electron microscopy and found to be greater than or equal to 90%, 96%, and 98% for equine PM, OAM, and IAM, respectively, and greater than or equal to 80%, 94%, and 97% for bovine PM, OAM, and IAM, respectively. GalTase activity was assayed under optimal conditions in all membrane preparations and was preferentially localized to the isolated PM both in equine and in bovine spermatozoa. The selective localization of GalTase to the sperm PM in two other species suggest that it may serve as a generalized gamete receptor during initial sperm-egg binding in mammals.

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Section: Materials and Methods Sperm Collection And Membrane Removalmentioning

confidence: 99%

Galactosyltransferase activity is restricted to the plasma membranes of equine and bovine sperm

Fayrer-Hosken

Caudle²,

Shur

1991

Molecular Reproduction Devel

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“…The recovery rate was recorded from the post-thaw motility of the spermatozoa. Acrosin activity was determined in acrosomal extracts of spermatozoa prepared according to SRIVASTAVA (1973) by detergent treatment. Enzyme activity was assayed spectrophotometrically, using a-N-benzyl-L-arginine ethyl ester (BAEE) as substrate (MEIZEL and MUKEKJI, 1975).…”

Section: Introductionmentioning

confidence: 99%

Acrosomal Damage of Buffalo Spermatozoa during Freezing in Extenders

Chinnaiya

Ganguli

2010

Zentralblatt Für Veterinärmedizin Reihe A

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IntroductionFreezing of semen in a suitable extender has opened a new vista in artificial insemination. It induces temporary quiescence in spermatozoa which can be stored for later resuscitation. Extensive data have shown that bovine spermatozoa can survive freeze-thawing when extended in glycerol-containing media (PICKETT and BERNDTSON, 1974;FOOTE, 1970). In contrast, limited success has been achieved in freezing buffalo semen (BHATTACHARYA and MAULE, 1962; BHOSREKAR, 1974), using the conventional egg-yolk citrate (EYC) diluent employed for bull semen; it is less effective for buffalo semen preservation. A new diluent, citric acid whey (CAW), has been recently evolved for buffalo semen preservation and freezing in this laboratory (GAN- GULI et al., 1973; GANGULI, 1974). The extracellular release of transaminase (CHINNAIYA et al., 1979) and the uptake of labelled glucose and fructose by buffalo spermatozoa in extenders during cold storage (ATREJA and GANGULI, 1978) have been reported. In the light of these observations, an assessment of the acrosomal damage in relation to leakage of acrosin of buffalo spermatozoa during freezing has been made when semen was extended in different media containing glycerol. This report presents results that indicate an almost similar efficiency of three diluents tested for freezing buffalo semen, as revealed by the acrosomal characteristics. Material and MethodsSemen samples were collected once a week from Murrah buffalo bulls using the artificial vagina technique (WALTON, 1945), two successive ejaculates being collected at a time. Semen quality was evaluated by the method of HERMAN and MADDEN (1953). Samples having high initial motility, with a score between 3.5 and 4.5 (5 being the best motility), determined microscopically, were split into three equal parts and diluted with CAW, EYC and Tris-'*

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“…Each time pooled semen was centrifuged at 9OOg for 15 min at 22°C to sediment spermatozoa. The sperm acrosomal extract was prepared as described by Srivastava (1973) and Srivastava et al (1974). Briefly, washed spermatozoa were stirred first with 0.05 M MgC1, in 0.05 M Tris Maleate, pH 6.1, at 2 4°C for 30 min and centrifuged.…”

Section: Preparation Of Acrosomal Extractsmentioning

confidence: 99%

Inhibition of rabbit sperm acrosomal enzymes by gossypol

Yuan

Shi

Srivastava

1995

Molecular Reproduction Devel

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The effect of gossypol on the activities of 10 acrosomal enzymes of the rabbit sperm was evaluated. Acrosin, Azocoll proteinase, neuraminidase, and arylsulfatase were significantly inhibited or completely inactivated by 12-76 microM gossypol. Hyaluronidase, beta-glucuronidase, and acid phosphatase were inhibited only at a higher concentration of gossypol (380 microM). Phospholipase C, alkaline phosphatase, and beta-N-Acetyl glucosaminidase were not inhibited even at 380 microM gossypol. Gossypol was found to be a noncompetitive inhibitor of arylsulfatase with a Ki of 120 microM. The inhibition was reversible and dose-dependent. As the acrosomal enzymes were more sensitive to the inhibition by gossypol compared to sperm enzymes involved in glycolysis or energy production, these assays may serve as a more reliable indicator for monitoring the occurrence of gossypol-induced sterility.

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Removal of Acrosomes of Ram and Rabbit Spermatozoa

Cited by 25 publications

References 9 publications

Galactosyltransferase activity is restricted to the plasma membranes of equine and bovine sperm

Galactosyltransferase activity is restricted to the plasma membranes of equine and bovine sperm

Acrosomal Damage of Buffalo Spermatozoa during Freezing in Extenders

Inhibition of rabbit sperm acrosomal enzymes by gossypol

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