Removal of anthropogenic lead pollutions by a potent Bacillus species AS2 isolated from geogenic contaminated site

Cephidian, Atefeh; Makhdoumi, Ali; Mashreghi, Mansour; Gharaie, Mohamad Hosein Mahmudy

doi:10.1007/s13762-016-1023-2

Cited by 16 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The residual Hg content in the respective culture supernatants after the growth of RWS2 was used to calculate the biological removal capacity (BRC) [55], expressed by the equation:

{\rm{BRC}}({\rm{mg}}\unicode{x02044}{\rm{g}})=(\Pr {\rm{imary}}\,{\rm{metal}}\,{\rm{concentration}}-{\rm{Residual}}\,{\rm{metal}}\,{\rm{concentration}})\unicode{x02044}({\rm{Bacterial}}\,{\rm{biomass}}\,{\rm{or}}\,{\rm{dry}}\,{\rm{weight}}).

…”

Section: Methodsmentioning

confidence: 99%

“…The biological removal efficiency or BRE was determined using the equation [55]:

\mathrm{BRE}\,( \% )=(\mathrm{Primary}\,\mathrm{metal}\,\mathrm{concentration}-\mathrm{Residual}\,\mathrm{metal}\,\mathrm{concentration})/(\mathrm{Primary}\,\mathrm{metal}\,{\rm{concentration}})\times 100.

…”

Section: Methodsmentioning

confidence: 99%

“…The residual Hg content in the respective culture supernatants after the growth of RWS2 was used to calculate the biological removal capacity (BRC) [55], expressed by the equation: The biological removal efficiency or BRE was determined using the equation [55]:…”

Section: Determination Of Resistance To Other Heavy Metalsmentioning

confidence: 99%

See 2 more Smart Citations

Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Saha,

Roy,

Saha

et al. 2023

J Basic Microbiol

View full text Add to dashboard Cite

Mercury (Hg) is a highly toxic heavy metal and Hg‐resistant indigenous bacterial isolates may offer a green and cost‐effective bioremediation strategy to counter Hg contamination. In this study, a potent Hg‐resistant bacterium was isolated from the forest soil of a bird sanctuary. Identification using matrix‐assisted laser desorption ionization‐time of flight mass spectrometry depicted the isolate as a strain of Bacillus tropicus, validated by morphological, biochemical, and molecular studies. The isolate demonstrated biological Hg removal efficiency and capacity of 50.67% and 19.76 mg g−1, respectively. The plasmid borne resistance determinant, merA, encoding mercuric reductase, was detected in the bacterium endowing it with effective Hg volatilization and resistance capability. A Fourier‐transform infrared spectroscopic comparative metabolic profiling revealed the involvement of various functional groups like –COOH, ‐CH2, ‐OH, PO4− and so on, resulting in differential spectral patterns of the bacterium both in control and Hg‐exposed situations. A temporal variance in metabolic signature was also observed during the early and mid‐log phase of growth in the presence of Hg. The bacterium described in this study is the first indigenous Hg‐resistant strain isolated from the Uttar Dinajpur region, which could be further explored and exploited as a potent bioresource for Hg remediation.

show abstract

“…The residual Hg content in the respective culture supernatants after the growth of RWS2 was used to calculate the biological removal capacity (BRC) [55], expressed by the equation:

{\rm{BRC}}({\rm{mg}}\unicode{x02044}{\rm{g}})=(\Pr {\rm{imary}}\,{\rm{metal}}\,{\rm{concentration}}-{\rm{Residual}}\,{\rm{metal}}\,{\rm{concentration}})\unicode{x02044}({\rm{Bacterial}}\,{\rm{biomass}}\,{\rm{or}}\,{\rm{dry}}\,{\rm{weight}}).

…”

Section: Methodsmentioning

confidence: 99%

“…The biological removal efficiency or BRE was determined using the equation [55]:

\mathrm{BRE}\,( \% )=(\mathrm{Primary}\,\mathrm{metal}\,\mathrm{concentration}-\mathrm{Residual}\,\mathrm{metal}\,\mathrm{concentration})/(\mathrm{Primary}\,\mathrm{metal}\,{\rm{concentration}})\times 100.

…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Saha,

Roy,

Saha

et al. 2023

J Basic Microbiol

View full text Add to dashboard Cite

show abstract

“…Much like other Gram-positive bacteria, Bacillus spp. also employ one or several of these methods to remove Pb from the contaminated environments [ 125 , 126 , 127 ] ( Table 1 ).…”

Section: Bacillus Species and Heavy Metalsmentioning

confidence: 99%

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

2021

View full text Add to dashboard Cite

The rise of anthropogenic activities has resulted in the increasing release of various contaminants into the environment, jeopardizing fragile ecosystems in the process. Heavy metals are one of the major pollutants that contribute to the escalating problem of environmental pollution, being primarily introduced in sensitive ecological habitats through industrial effluents, wastewater, as well as sewage of various industries. Where heavy metals like zinc, copper, manganese, and nickel serve key roles in regulating different biological processes in living systems, many heavy metals can be toxic even at low concentrations, such as mercury, arsenic, cadmium, chromium, and lead, and can accumulate in intricate food chains resulting in health concerns. Over the years, many physical and chemical methods of heavy metal removal have essentially been investigated, but their disadvantages like the generation of chemical waste, complex downstream processing, and the uneconomical cost of both methods, have rendered them inefficient,. Since then, microbial bioremediation, particularly the use of bacteria, has gained attention due to the feasibility and efficiency of using them in removing heavy metals from contaminated environments. Bacteria have several methods of processing heavy metals through general resistance mechanisms, biosorption, adsorption, and efflux mechanisms. Bacillus spp. are model Gram-positive bacteria that have been studied extensively for their biosorption abilities and molecular mechanisms that enable their survival as well as their ability to remove and detoxify heavy metals. This review aims to highlight the molecular methods of Bacillus spp. in removing various heavy metals ions from contaminated environments.

show abstract

“…In polluted sites, some indigenous microorganisms can develop a variety of resistance and detoxification mechanisms to neutralize/tolerate heavy metal stress for survival (Bruins et al 2000;Cephidian et al 2016). Prevention of the entry of metal ions into microbial cells by extracellular sequestration of metals through functional groups on the cell walls and some intracellular detoxification mechanisms (such as the production of some metal-chelating substances) as well as metal efflux mediated by ATPase pumps are probably responsible for the resistance of the isolate to heavy metals (Hall 2002;Zhang et al 2006;Vijayaraghavan and Yun 2008;Mejias Carpio et al 2017).…”

Section: Identification Of the Cu-and Cd-resistant Bacterial Strain Dc-b3mentioning

confidence: 99%

Characterization of Cu and Cd biosorption by Pseudomonas sp. strain DC-B3 isolated from metal mine soil

Liang

Chen

Mei

et al. 2018

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

A Cu-and Cd-resistant bacterial strain, DC-B3, was isolated from heavy metal-contaminated soils in a Cu mine, was identified as belonging to Pseudomonas sp. and biosorbed Cu 2+ and Cd 2+ from aqueous solution in the present study. Its biosorption properties as a function of environmental parameters such as contact time, initial concentration, pH, temperature and bacterial dosage were investigated through batch experiments, and the removal capacity peaked at pH 6.0, 40 °C and 0.5 g biomass L −1 at an initial concentration of 105 mg L −1 . Sorption occurred extremely rapidly at the beginning, equilibrium was reached after approximately 1 h and 4 h for Cu 2+ and Cd 2+ , respectively, and the biosorption kinetic data for both metals were well fitted by the pseudo-second-order model (r 2 > 0.99). Sorption isotherms showed that the adsorptions of both metals were highly favourable, and equilibrium data were better described by the Langmuir model (r 2 > 0.98 for Cu 2+ and 0.99 for Cd 2+ ), with the estimated maximum sorption capacities of 12.6 and 26.5 mg g −1 for Cu 2+ and Cd 2+ , respectively. Desorption assays suggested that biosorption was a chemical process and that a fraction of the metal ions might have entered the bacterial cells. Fourier transform infrared spectroscopy analysis revealed that hydroxyl, amino, carboxyl and phosphate functional groups on the bacterial cell walls might be mainly responsible for the biosorption, although their contributions to the binding of Cu 2+ and Cd 2+ were different. This result implied that the strain DC-B3 can be used as a potentially eco-friendly and efficient biosorbent for the remediation of Cu(II) and Cd(II) contaminants.

show abstract

Removal of anthropogenic lead pollutions by a potent Bacillus species AS2 isolated from geogenic contaminated site

Cited by 16 publications

References 37 publications

Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Study of mercury resistance and Fourier transform infrared spectroscopy‐based metabolic profiling of a potent Bacillus tropicus strain from forest soil

Unraveling the Underlying Heavy Metal Detoxification Mechanisms of Bacillus Species

Characterization of Cu and Cd biosorption by Pseudomonas sp. strain DC-B3 isolated from metal mine soil

Contact Info

Product

Resources

About