Removal of aqueous phenol catalysed by a low purity soybean peroxidase

Wilberg, Katia de Quadros; Assenhaimer, Cristhiane; Rubio, J.

doi:10.1002/jctb.646

Cited by 48 publications

(23 citation statements)

References 21 publications

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“…Authors have reported the use of purified horseradish peroxidase (HRP) to remove 30 different phenols and aromatics amines (Wilberg et al 2000;Cooper and Nicell 1996). Phenol conversion is activated by H 2 O 2 ; the enzyme catalyzes the oxidation of aromatic compounds, forming free radicals which undergo spontaneous polymerisation (Wilberg et al 2002). Soybean seed hulls have been identified as a rich source of peroxidase, the soybean peroxidase (SBP), and being a by-product of soybean food industry, they provide a cheap and abundant source of peroxidase (Wilberg et al 2002;Hejri and Saboora 2009).…”

Section: Enzymatic Polymerization Of Phenolmentioning

confidence: 99%

“…Phenol conversion is activated by H 2 O 2 ; the enzyme catalyzes the oxidation of aromatic compounds, forming free radicals which undergo spontaneous polymerisation (Wilberg et al 2002). Soybean seed hulls have been identified as a rich source of peroxidase, the soybean peroxidase (SBP), and being a by-product of soybean food industry, they provide a cheap and abundant source of peroxidase (Wilberg et al 2002;Hejri and Saboora 2009). Radish roots contain peroxidase enzyme and can be used for the removal of phenol from wastewaters (Naghibi et al 2003).…”

Section: Enzymatic Polymerization Of Phenolmentioning

confidence: 99%

“…Treatment in the presence of PEG as an additive increased the effect of enzymatic removal. Wilberg et al (2002) studied the application of low-purity soybean peroxidase (LP-SBP), obtained from wasted seed hulls, as a catalyst for phenol polymerizations in an aqueous solution in the presence of hydrogen peroxide. At all phenol concentrations tested, a retention time of about 100 min was sufficient to achieve good results.…”

Section: Momordica Charantiamentioning

confidence: 99%

See 2 more Smart Citations

Biological removal of phenol from wastewaters: a mini review

Pradeep¹,

Anupama²,

Navya³

et al. 2014

Appl Water Sci

176

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Phenol and its derivatives are common water pollutants and include wide variety of organic chemicals. Phenol poisoning can occur by skin absorption, inhalation, ingestion and various other methods which can result in health effects. High exposures to phenol may be fatal to human beings. Accumulation of phenol creates toxicity both for flora and fauna. Therefore, removal of phenol is crucial to perpetuate the environment and individual. Among various treatment methods available for removal of phenols, biodegradation is environmental friendly. Biological methods are gaining importance as they convert the wastes into harmless end products. The present work focuses on assessment of biological removal (biodegradation) of phenol. Various factors influence the efficiency of biodegradation of phenol such as ability of the microorganism, enzymes involved, the mechanism of degradation and influencing factors. This study describes about the sources of phenol, adverse effects on the environment, microorganisms involved in the biodegradation (aerobic and anaerobic) and enzymes that polymerize phenol.

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Section: Enzymatic Polymerization Of Phenolmentioning

confidence: 99%

Section: Enzymatic Polymerization Of Phenolmentioning

confidence: 99%

Section: Momordica Charantiamentioning

confidence: 99%

See 1 more Smart Citation

Biological removal of phenol from wastewaters: a mini review

Pradeep¹,

Anupama²,

Navya³

et al. 2014

Appl Water Sci

176

View full text Add to dashboard Cite

show abstract

“…Above all, SBP can be certainly cheaper than TP, since it is easily extracted from soybean seed coats, which are a waste product of the soybean processing industry (Wilberg et al, 2002). A crude SBP extract has been found to be more efficient than the purified one (Flock et al, 1999), as are the peroxidases cited earlier, and it is active over a broad range of pH (3·5-8·0) (Caza et al, 1999;Kamal and Behere, 2003;Wright and Nicell, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Peroxidases catalyse the oxidisation of phenols (Al-Kassim et al, 1994;Caza et al, 1999;Wilberg et al, 2002;Yu et al, 1994) and anilines (Saha et al, 2008) to form aromatic radicals in the presence of hydrogen peroxide (H 2 O 2 ). These radicals diffuse from the active site of the enzyme into the solution, where they couple non-enzymatically to form dimers.…”

Section: Introductionmentioning

confidence: 99%

Soybean peroxidase-catalysed removal of benzidines from water

Altahir

FengWei

Hassan

et al. 2015

Journal of Environmental Engineering and Science

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Crude soybean peroxidase (SBP), isolated from soybean seed coats (hulls) at unusually low concentrations, catalyses the oxidative polymerisation of hazardous aqueous benzidine and its 3,3′-dichloro, 3,3′-dimethyl and 3,3′-dimethoxy derivatives in the presence of hydrogen peroxide. The optimum operating conditions for oxidation of 0·10 mM benzidine were investigated. At pH 5, the hydrogen peroxide-to-substrate concentration ratio was 1·5 and the minimum SBP concentration required to achieve at least 95% conversion of the benzidine in synthetic wastewater was 0·43 mU/ml. Progress curves were established for the conversion of the four substrates, and apparent first-order rate constants were derived. Enzyme-catalysed polymerisation with SBP and subsequent removal of the polymeric products generated can provide an alternative means to conventional methods for treating many aromatic wastewater pollutants, including the benzidines studied here.

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Synthesis of peroxidase‐encapsulated sodium cellulose sulphate/poly‐dimethyl‐diallyl‐ammonium chloride biopolymer via polyelectrolyte complexation for enhanced removal of phenol

Chiong

Lau

Zeng

et al. 2019

Asia-Pacific J Chem Eng

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Peroxidases have attracted significant interests in enzymatic wastewater treatment strategies. In this work, jicama peroxidase (JP) was extracted from jicama skin peels and used for the degradation of phenol under free and immobilized conditions. The crude enzyme extract demonstrated enzymatic activity of 1.6 ± 0.1 U mL −1 . Sodium cellulose sulphate/poly-dimethyl-diallyl-ammonium chloride (NaCS-PDMDAAC) spherical capsules were synthesized and immobilized with crude JP to generate JP beaded capsules with an average diameter of 5.05 mm ± 0.16 mm. Phenol biodegradation analysis showed that the free and immobilized JP capsules demonstrated optimum working pH values of 7 and 6, respectively, and both systems maintained JP catalytic functionalities over a broad range of H 2 O 2 concentration before H 2 O 2 inhibition.The optimal temperature range for phenol removal was from 25°C to 40°C for both free and immobilized JP with lower removal efficiency above 45°C due to thermal denaturation. Due to diffusive mass transfer limitation, immobilized JP capsules required a longer reaction time of 15 hr for optimal phenol removal efficiency of >95%, whereas free JP achieved the same efficiency in 13 hr. The first order kinetic rate constants for free and immobilized JP capsules were determined to be 1.21 hr −1 and 1.02 hr −1 , respectively. JP capsules maintained reusability up to 4 cycles at the highest removal efficiency of >95% with no regeneration.

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Removal of aqueous phenol catalysed by a low purity soybean peroxidase

Cited by 48 publications

References 21 publications

Biological removal of phenol from wastewaters: a mini review

Biological removal of phenol from wastewaters: a mini review

Soybean peroxidase-catalysed removal of benzidines from water

Synthesis of peroxidase‐encapsulated sodium cellulose sulphate/poly‐dimethyl‐diallyl‐ammonium chloride biopolymer via polyelectrolyte complexation for enhanced removal of phenol

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