Removal of bacteria from boar semen using a low-density colloid

Morrell, Jane M.; Núñez-González, A.; Crespo-Félez, I.; Martínez-Martínez, Sonia; Alborcia, M. J. Martínez; Fernández‐Alegre, Estela; Domínguez, Juan Carlos; Gutiérrez-Martín, César B.; Martínez‐Pastor, Felipe

doi:10.1016/j.theriogenology.2018.12.028

Cited by 42 publications

(33 citation statements)

References 31 publications

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“…The components of the commercially available extender, particularly antibiotics, are not available on the manufacturer's website (IMV-Technologies website). However, even on day 7 in the present study, the membrane integrity was higher in all samples than in the previous study with 40% Porcicoll (Morrell et al, 2019).…”

Section: Discussioncontrasting

confidence: 93%

Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality

Deori

Johannisson

Morrell

2020

Reprod Domestic Animals

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Artificial insemination (AI) was developed as a technique for effectively distributing gametes from males of high genetic merit while avoiding movement of animals between herds, and inter-animal contact. However, potential pathogens can be spread between animals in semen. Semen from healthy animals frequently contains bacteria that contaminate the ejaculate during semen collection and processing. Antibiotics are added to semen extenders used to prepare semen doses for artificial insemination (AI), to

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Section: Discussioncontrasting

confidence: 93%

Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality

Deori

Johannisson

Morrell

2020

Reprod Domestic Animals

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“…Myroides species have been cultured from blood, 3 urine, 8 soft tissue, 9 bronchoalveolar lavage, 10 sputum, 11 bile, 12 pus 13 and pericardial fluid, 14 although rarely isolated from clinical specimens. They have also been found in seafood products, meat-processing, flesh flies, grey mullet's gut, livestock manure, Sagor catfish, and boar semen, [15][16][17][18] bacteria existing in these non-human hosts may have some connection with the transmission of the pathogen. Myroides infections include cellulitis, urinary tract infection (UTI), bacteremia, necrotizing fasciitis, pneumonia, pericarditis endocarditis, ventriculitis, acalculous cholecystitis, urosepsis, empyema and canaliculitis as well as nosocomial outbreaks.…”

Section: Discussionmentioning

confidence: 99%

<p>A Confirmed Catheter-Related Blood Stream Infection (CRBSI) in an Immunocompetent Patient Due to <em>Myroides odoratimimus</em>: Case Report and Literature Review</p>

Lu¹,

Xia²,

Zhang³

et al. 2020

IDR

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The genus Myroides are gram-negative bacilli which are completely aerobic, nonmotile, non-fermenting and yellow-pigmented with a characteristic fruity odor. Myroides species are widely found in the environment, especially in water and soil, and are considered as low-grade opportunistic pathogens for humans. Myroides infections are most commonly seen in immunocompromised patients and only rarely occur in immunocompetent patients. We here report the first confirmed catheter-related bloodstream infection (CRBSI) due to Myroides odoratimimus in an immunocompetent patient. We also review the literature related to Myroides infections.

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“…Recently, the possibility of separating all sperm from seminal plasma without affecting the semen quality was investigated in swine (Morrell et al, 2019). The ejaculates were diluted in the antimicrobial-free Beltsville Thawing Solution and subjected to single-layer centrifugation in a low-density colloid, which provided an increase on sperm velocity and linearity, besides removing or reducing bacterial contamination in boar ejaculates.…”

Section: Physical Methodsmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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How to cite: Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology. AbstractThe use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Among the various mammalian species, there is a large variation regarding the antimicrobial types added to semen extenders as cephalosporins (ceftiofur, cefdinir, eg) and quinolones (ofloxacin, ciprofloxacin), alone or in combination with large action spectra substances as penicillin-streptomycin and gentamicin-tylosin-lincomycin-spectinomycin. To combat problems related to bacterial resistance to these drugs, the emergence of alternatives is increasingly evident. Among these alternatives, use of physical methods as centrifugation and filtration, as well as the use of antimicrobial peptides and other substances from different origins have been highlighted for presenting antimicrobial potential.

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Removal of bacteria from boar semen using a low-density colloid

Cited by 42 publications

References 31 publications

Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality

Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality

<p>A Confirmed Catheter-Related Blood Stream Infection (CRBSI) in an Immunocompetent Patient Due to <em>Myroides odoratimimus</em>: Case Report and Literature Review</p>

Current and alternative trends in antibacterial agents used in mammalian semen technology

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