Removal of bovine viral diarrhea virus (BVDV) from lumpy skin disease virus (LSDV) vaccine stocks by passage on chorioallantoic membranes of fertilized hens’ eggs

Munyanduki, Henry; Omar, Ruzaiq; Douglass, Nicola; Williamson, Anna‐Lise

doi:10.1016/j.jviromet.2019.113752

Cited by 8 publications

(12 citation statements)

References 19 publications

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“…Once confirmed, the recombinant was grown from a single focus in a 96-well plate and scaled up in a hyperflask of MDBK cells, which were infected at an MOI of 0.2. As MDBK cells harbor bovine viral diarrheal virus (BVDV), the vaccine to be used for the cattle experiment was grown on chick chorioallantoic membranes (CAMs) [22].…”

Section: Isolation Of Nlsdv∆sod-uctmentioning

confidence: 99%

“…The lysate was passaged on MDBK cells and three rounds of picking of non-fluorescing foci were performed. A single non-fluorescing focus was isolated on a 96-well plate and expanded in MDBK cells followed by passage on chick CAMs [22].…”

Section: Isolation Of Nlsdvsodis-uctmentioning

confidence: 99%

“…Viruses nLSDV, nLSDV∆SOD-UCT and nLSDVSODis-UCT were compared for growth on chick CAMs. Seven-day-old specific pathogen free eggs (SPF) from Leghorn chickens (Avifarms (Pty) Ltd. (Lyttelton, South Africa)) were infected as previously described [22,24]. CAMs were inoculated with 100 µl of 10 4 TCID 50 / mL nLSDV, nLSDV∆SOD-UCT and nLSDVSODis-UCT.…”

Section: Growth Curves On Chick Chorioallantoic Membranes (Cams)mentioning

confidence: 99%

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Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

et al. 2020

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Lumpy skin disease is an important economic disease of cattle that is controlled by vaccination. This paper presents an investigation into the role of the lumpy skin disease virus (LSDV) superoxide dismutase (SOD) homologue on growth and histopathology of the virus both in vitro and in vivo. SOD homologue knock-out and knock-in recombinants (nLSDV∆SOD-UCT and nLSDVSODis-UCT, respectively) were constructed and compared to the Neethling vaccine (nLSDV) for growth in a permissive bovine cell line as well as on fertilized chick chorioallantoic membranes (CAMs). The infected CAMs were scored for histological changes. Deletion of the SOD homologue from LSDV reduced virus growth both in Madin-Darby bovine kidney (MDBK) cells as well as on CAMs. Furthermore, the knockout virus showed reduced inflammation in CAMs and more ballooning degeneration. A pilot experiment was performed in cattle to compare the lesions produced by the different LSDV constructs in the same animal. One animal developed a larger lesion to nLSDV∆SOD-UCT compared to both nLSDVSODis-UCT and nLSDV. Histological analysis of biopsies of these lesions shows less inflammation and necrosis associated with nLSDVSODis-UCT compared to nLSDV and nLSDV∆SOD-UCT. None of the vaccinated animals showed disseminated LSDV disease, indicating that the candidate vaccines are safe for further testing. Our results suggest that the SOD homologue may improve immunogenicity and reduce virulence.

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Section: Isolation Of Nlsdv∆sod-uctmentioning

confidence: 99%

Section: Isolation Of Nlsdvsodis-uctmentioning

confidence: 99%

Section: Growth Curves On Chick Chorioallantoic Membranes (Cams)mentioning

confidence: 99%

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Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

et al. 2020

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“…Virus BVD merupakan patogen pada sapi, sehingga bila pada vaksin tersebut terdapat virus LSD dan BVD, akan menjadi tidak efektif karena dapat menimbulkan penyakit BVD. Munyanduki et al (2020) telah menemukan cara agar menghilangkan virus BVD saat propagasi virus LSD untuk pembuatan vaksin, yaitu dengan cara dikembangkan melalui sel unggas. Unggas bersifat nonpermisif terhadap virus BVD, tetapi permisif terhadap LSDV.…”

Section: Vaksinasiunclassified

“…Seed virus LSD dipasase ulang pada CAM sebelum ditanam dalam sel primary lamb testes (LT) untuk diperbanyak. Seed vaksin tetap negatif BVDV setelah diteruskan ke sel LT (Munyanduki et al 2020).…”

Section: Vaksinasiunclassified

Lumpy Skin Disease: Ancaman Penyakit Emerging Bagi Kesehatan Ternak Sapi Di Indonesia

Sendow¹,

Assadah²,

Ratnawati³

et al. 2021

WARTAZOA

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Lumpy skin disease (LSD) menyebabkan kerugian ekonomi bagi peternak sapi, yang disebabkan oleh virus LSD, genus Capripoxvirus, famili Poxviridae. Penyakit ini di karakterisasi dengan adanya nodul pada kulit. Virus ini hanya menginfeksi sapi dan kerbau rawa yang memiliki tingkat mortalitas rendah, namun tingkat morbiditas tinggi. Sedangkan virus ini tidak menginfeksi kambing dan domba. Hingga saat ini, LSD belum pernah dilaporkan di Indonesia, oleh karena itu pengenalan penyakit LSD diperlukan terutama bagi dokter hewan dan paramedis lapangan sehingga infeksi LSD dapat diketahui dan penanganannya dapat dilakukan lebih dini. Tulisan ini akan membahas mengenai penyakit LSD, cara penyebaran penyakit, epidemiologi, diagnosis, faktor risiko serta pengendalian penyakit LSD, sehingga diharapkan masuknya infeksi LSD dapat terdeteksi sedini mungkin dan diantisipasi dengan lebih arif oleh pemangku kebijakan.

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Experimental infection of indigenous North African goats with goatpox virus

et al. 2021

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Background Goatpox is a viral disease caused by infection with goatpox virus (GTPV) of the genus Capripoxvirus, Poxviridae family. Capripoxviruses cause serious disease to livestock and contribute to huge economic losses. Goatpox and sheeppox are endemic to Africa, particularly north of the Equator, the Middle East and many parts of Asia. GTPV and sheeppox virus are considered host-specific; however, both strains can cause clinical disease in either goats or sheep with more severe disease in the homologous species and mild or sub-clinical infection in the other. Goatpox has never been reported in Morocco, Algeria or Tunisia despite the huge population of goats living in proximity with sheep in those countries. To evaluate the susceptibility and pathogenicity of indigenous North African goats to GTPV infection, we experimentally inoculated eight locally bred goats with a virulent Vietnamese isolate of GTPV. Two uninfected goats were kept as controls. Clinical examination was carried out daily and blood was sampled for virology and for investigating the antibody response. After necropsy, tissues were collected and assessed for viral DNA using real-time PCR. Results Following the experimental infection, all inoculated goats displayed clinical signs characteristic of goatpox including varying degrees of hyperthermia, loss of appetite, inactivity and cutaneous lesions. The infection severely affected three of the infected animals while moderate to mild disease was noticed in the remaining goats. A high antibody response was developed. High viral DNA loads were detected in skin crusts and nodules, and subcutaneous tissue at the injection site with cycle threshold (Ct) values ranging from 14.6 to 22.9, while lower viral loads were found in liver and lung (Ct = 35.7 and 35.1). The results confirmed subcutaneous tropism of the virus. Conclusion Clinical signs of goatpox were reproduced in indigenous North African goats and confirmed a high susceptibility of the North African goat breed to GTPV infection. A clinical scoring system is proposed that can be applied in GTPV vaccine efficacy studies.

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Removal of bovine viral diarrhea virus (BVDV) from lumpy skin disease virus (LSDV) vaccine stocks by passage on chorioallantoic membranes of fertilized hens’ eggs

Cited by 8 publications

References 19 publications

Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

Lumpy Skin Disease: Ancaman Penyakit Emerging Bagi Kesehatan Ternak Sapi Di Indonesia

Experimental infection of indigenous North African goats with goatpox virus

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