The present study focused on the effect of hot isostatic pressing (HIP) treatment on the microstructure of K417G superalloy. The experimental results showed that after the HIP treatment the size and volume fraction of the porosities significantly decreased. In addition, the dendritic structure and γ/γ' eutectics in the as-cast specimens became obscure after the HIP treatment due to the improvement of segregation. The γ′ phases in the dendrite core were smaller than those in the interdendritic region, whether in the as-cast or HIP specimens. The slow cooling at the end of the HIP treatment leads to the irregular morphology of the γ′ phases.