Removal of Cd (II) from Aqueous Solution using Immobilized Rhizomucor Tauricus

Kumar, K. Kishore; Prasad, M.M.; Sarma, G. V. S.; Murthy, Ch. V. R.

doi:10.4172/1948-5948.1000004

Cited by 25 publications

(2 citation statements)

References 54 publications

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“…In most of them, VPA is monitored by fluorescence polarization immunoassay (FPIA). [7,8] However other methods, such as high-performance liquid chromatography (HPLC) assay coupled with either ultraviolet, [9,10] fluorescence detection, [11][12][13] or gas chromatography with mass spectrometric detection [14,15] have also led to satisfactory results. Most of these techniques require laborious sample preparation with evaporation steps or derivatization.…”

Section: Introductionmentioning

confidence: 98%

Determination of Total and Unbound Concentrations of Valproic Acid in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Fernández-Campos

Calpena

Soy

et al. 2012

Journal of Liquid Chromatography & Related Technologies

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& An analytical method for the determination of valproic acid in human plasma and ultrafiltrated human plasma has been developed and validated. The analytical method lies in the solid phase extraction of valproic acid (VPA), followed by its quantification through an LC-MS method, using benzoic acid as the internal standard. The intra-assay and inter-assay precisions of this technique ranged between 4.29% and 9.91% and its accuracy ranged between 1.00% and 7.73%, for VPA spiked in plasma matrix. For VPA spiked in ultrafiltrated plasma, matrix precisions were from 6.29% to 9.84% and accuracies, from À1.17% to 5.38%. The limit of quantification was set at 1 lg=mL (precision 3.20%, accuracy 11.31%). The linear working range of concentrations was established between 10 and 250 lg=mL for the plasma matrix and between 1 and 100 lg=mL for the ultrafiltrated plasma matrix. The absolute recovery of valproic acid was over 82% in the studied range of concentrations in both matrixes. Valproic acid was found to be stable in both matrixes at the assayed conditions. This method has shown to be suitable for the analysis of free and total valproic acid concentrations in human plasma samples.

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Section: Introductionmentioning

confidence: 98%

Determination of Total and Unbound Concentrations of Valproic Acid in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Fernández-Campos

Calpena

Soy

et al. 2012

Journal of Liquid Chromatography & Related Technologies

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“…Various bioanalytical methods of VPA which included high-performance liquid chromatography (HPLC) 9 had been developed, but it consumed significant analysis time and large amounts of samples. In recent years, simple, selective and highly sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) were developed and validated.…”

Section: 5mentioning

confidence: 99%

Rapid and Sensitive Analysis of Valproic Acid in Human Red Blood Cell by LC-MS/MS

Han¹,

Kim²,

Jeon³

et al. 2012

Bulletin of the Korean Chemical Society

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A sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine valproic acid in human red blood cell (RBC). It is important to measure the drug concentration of the RBC as well as that of the plasma because of drug partitioning for pharmacokinetic and pharmacodynamic study. The method was linear over the dynamic range of 1-100 µg/mL with a correlation coefficient r = 0.9997. The linearity of this method was established from 1 to 100 µg/mL for valproic acid in red blood cell with accuracy and precision within 15% at all concentrations. The intra-run and inter-run assay accuracy and coefficient of variations are all within 15% for all QC samples prepared in plasma and red blood human samples. Then, valproic acid amount by protein precipitation in plasma was quantified by LC-MS/MS mass spectrometry. The distribution ratio of VPA in RBC and plasma was analyzed by clinical samples. Based on measurement of the valproic acid in human red blood cell, this method has been applied to clinical research for study of distribution ratio of valproic acid in blood.

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