Removal of cells from a malignant B-cell line from bone marrow with immunomagnetic beads and with complement and immunoglobulin switch variant mediated cytolysis

CD34 selection for purging in multiple myeloma and analysis of CD34+b cell precursors

“…Negative selection resulted in tumor cell depletion over a range of 0.1 to 4 log in a recent clinical study [20] and was higher than 4 log experimentally [21]. We obtained a comparable myeloma cell depletion of log 3.97 by selection of CD34' cells.…”

“…In lymphohematopoietic malignancies and solid tumors, residual tumor cells in the bone marrow may play a crucial role in relapse of the disease after high-dose therapy and autografting [l, 21. Various purging procedures have been used to separate malignant cells from normal hematopoietic progenitors.…”

Effective removal of SCLC cells from human bone marrow. Use of four monoclonal antibodies and immunomagnetic beads

“…Altogether, the results in the present work were better than any of those previously reported, probably reflecting both the efficacy of immunomagnetic procedures (Kiesel et al, 1987;Kvalheim et al, 1988) and the properties of the antibodies used. In agreement with the experience of Vredenburgh & Ball (1990), the best results were obtained with two purging cycles at a 50:1 ratio of beads to tumour cells.…”

Kinetic Study of the Degradation of Crystal Violet by K2S2O8. Comparison with Malachite Green

“…In order to study the cytotoxic effect of crystal violet on human cells, and if the pretreatment with K 2 S 2 O 8 (KSP) protects against its cytotoxicity, two Blymphocytic cell lines were used: SU-DHL-4 and OCI-LY-1 [43][44][45]. Both cell lines were cultured in suspension in RPMI-1640 media which was supplemented with 10 % heat inactivated fetal bovine serum (FBS), L-glutamine (100x GlutaMaX, Gibco/Invitogen 35050), penicillin, streptomycin (100xPen/strep, Gibco/ Invitrogen 15070-063) at 37 o C and 5 % CO 2 .…”