Removal of chlorophenols from wastewater by immobilized horseradish peroxidase

Takagi, Kenji; Wada, Shinpei; Ichikawa, Hiroyasu

doi:10.1002/(sici)1097-0290(19960705)51:1<126::aid-bit15>3.0.co;2-o

Cited by 156 publications

(68 citation statements)

References 18 publications

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“…In HRP-catalysed phenolic reactions, the HRP inactivation mainly occurs as a result of sorption/occlusion by polymeric products (HRP adsorption on precipitated products occludes its active sites) [44] or upon heme-destruction (generated free radicals react and inactivate the heme-centre in HRP) [45]. This improvement in the efficiency of HRP by PEG addition during dephenolization is due to the formation of a protective layer in the vicinity of the active centre of HRP which restricts the attack of free phenoxy radicals and help in effectively suppressing the heme-destruction by co-dissolved PEG [44][45][46].…”

Section: Efficient Removal Of Phenol and P-chlorophenol From Spiked Mmentioning

confidence: 99%

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Ahirwar¹,

Sharma²,

Singh³

et al. 2017

JMSE-B

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Abstract:In the light of the increasing burden of pollutants in major rivers and stringent environmental legislation, adaptation to eco-friendly treatment approaches is desperately required to decontaminate industrial effluents before its discharge to rivers and other fresh water-bodies. In present study, we have designed a simple and efficient method for removal of phenols from effluent wastewater using an immobilized preparation of HRP (horseradish peroxidase). The enzyme was isolated in bulk amount from the roots of the Armoracia rusticana and covalently immobilized to polycarbonate supports using a photolabile linker FNAB (1-fluoro-2-notro-4-azidobenzene). The immobilized enzyme showed enhanced storage stability and reusability. The immobilized HRP was subsequently used for degradation of phenols in sewage and spiked wastewater. The phenol content of spiked wastewater was reduced to 93% in the 3 L reactor following treatment with immobilized HRP and H 2 O 2 . The improvement in the quality of water upon treatment was reflected by the changes in pH, conductivity, TDS (total dissolved salts) and biodegradation of organic contents as indicated by 77% and 87% reduction in COD (chemical oxygen demand) and BOD (biochemical oxygen demand) respectively in the analyzed sample.

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Section: Efficient Removal Of Phenol and P-chlorophenol From Spiked Mmentioning

confidence: 99%

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Ahirwar¹,

Sharma²,

Singh³

et al. 2017

JMSE-B

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“…Polyethylene glycol is biodegradable and as an additive improved the removal efficiency by protecting the enzyme during the reaction [52][53][54][55][56]. In this study the presence of PEG remarkably improved the catalytic performance of I-PGP in the oxidation of phenol and α-naphthol.…”

Section: Calculated Value (A-b=c) (X) 630mentioning

confidence: 99%

Application of Diethylaminoethyl Cellulose Immobilized Pointed Gourd (Trichosanthes dioica) Peroxidase in Treatment of Phenol and α-Naphthol

Singh¹

2015

J Bioprocess Biotech

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Recently, enzymatic treatment using peroxidases in removal of aromatic compounds has gained importance. In this study pointed gourd peroxidase was salt fractionated and direct immobilization of these proteins on diethylaminoethyl cellulose for oxidation of phenol and α-naphthol has been investigated. The activated diethylaminoethyl cellulose was quite effective in high yield immobilization of peroxidases from pointed gourd and it could bind ~576 units per g of the matrix. Immobilized pointed gourd peroxidase on this anion exchanger showed very high effectiveness factor 'Ɛ' as 0.91 with an activity yield of 91%. Immobilized PGP (I-PGP) as compared to soluble counterparts (s-PGP) were more effective and removed 79%, 88% and 54% oxidation of phenol and α-naphthol by 75%, 81% and 61% at 30, 40 and 50°C respectively, with a treatment time of 140 min. In the absence CdCl2 s-PGP as well as I-PGP exhibited upto 93% of oxidation of these compounds; whereas the presence of CdCl2 of negatively affected the removal of phenol and α-naphthol. The reactor worked well continuously for over one month for effectively oxidizing/ removing phenol and α-naphthol by 54% and 61% respectively. Thus, such immobilized enzyme systems in reactor have a great future and could be exploited for treating organic pollutants present in industrial effluents.

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“…Therefore we used RB 19 for enzymatic decolorization [10]. It structurally resembles certain polycyclic aromatic hydrocarbons, which are substrates of ligninolytic peroxidases [24][25][26]. It has a structure consisting of azo, anthrazine, naphthalene and sulfonated groups (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Determination of decolorization properties of Reactive Blue 19 dye using Horseradish Peroxidase enzyme

Celebi¹,

Altıkatoğlu²,

Mustafaeva³

et al. 2013

Turk J Bioch

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Aim: Fenolic compounds are major of pollutants in medicine, food and local matrixe. The enzyme peroxidase is known for its capacity to remove phenolic compounds and aromatic amines from aqueous solutions and also to decolorize textile effluents. In this study, decolorization of anthraquinone dye, Reactive Blue 19 (RB 19), large quantities of which used in textile and other industries was investigated using Horseradish peroxidase (HRP) at different conditions. In addition, decolorization efficiency of dye by enzyme was studied in the presence of denaturing agent urea and salt in synthetic wastewater. Material and Methods: Horseradish peroxidase and Reactive Blue 19 were obtained by commercial way. The enzymatic decolorization of the dye was examined by UV-Vis spectrophotometer measurements. Decolorization studies were performed by varying parameters such as enzyme and dye concentrations, pH, temperature, incubation time, presence of H 2 O 2 , salt and urea. Results: Optimum pH value for dye decolorization was determined as 5.0. Maximum dye was removed within 5 min after the beginning for every experiment. After 5 minutes of treatment, the color removal of dye was ca. 90-95% at pH 5.0 and all temperatures. Furthermore, the higher concentrations of dye, H 2 O 2 and NaCl did not exhibit inhibition effect but the initial decolorization rate decreased with increasing the urea concentration. The kinetic constants of enzyme were determined for dye.

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Removal of chlorophenols from wastewater by immobilized horseradish peroxidase

Cited by 156 publications

References 18 publications

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Application of Diethylaminoethyl Cellulose Immobilized Pointed Gourd (Trichosanthes dioica) Peroxidase in Treatment of Phenol and α-Naphthol

Determination of decolorization properties of Reactive Blue 19 dye using Horseradish Peroxidase enzyme

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