-In this work, the biosorption of Cr 3+ and Ni 2+ by Sargassum filipendula pre-treated with CaCl 2 was studied. Kinetic and equilibrium experiments were carried out for mono-and multi-component solutions in a batch reactor at pH 3.0 and 30 ºC. The results from the kinetic experiments showed that Cr 3+ adsorbs slower than Ni 2+ . This behavior was explained by means of a mechanistic analysis, which showed that Cr 3+ uptake presented three adsorption stages, whereas Ni 2+ adsorption presents only two. The mono-component equilibrium data, along with binary kinetic data obtained from mono-component experiments, showed that, although the kinetics for Cr 3+ removal are slower, the biomass had a stronger affinity for this ion. Almost all Ni 2+ is desorbed from the biomass as Cr 3+ adsorbs. The binary equilibrium data also presented this behavior. The binary data was also modeled by using modified forms of the Langmuir, Jain and Snoeyink, and Langmuir-Freundlich isotherms. However, the prediction obtained presented low accuracy. An alternative modeling with artificial neural networks was presented and the results showed that this technique could be a promising tool to represent binary equilibrium data. The main contribution of this work was to obtain experimental data for Cr 3+ /Ni 2+ adsorption, which is a system rarely found in the literature and that provides information that could be used in process modeling and simulation.