Removal of detergent and solvent from solvent–detergent-treated immunoglobulins

Trešćec, Anđa; Šimić, Mirjana; Branović, Karmen; Gebauer, Branka; Benko, Bojan

doi:10.1016/s0021-9673(99)00178-8

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…Most inactivation usually occurs within the first 30-60 minutes of the S/D treatment (49) . S/D treatment was performed by following the method of Horowitz et al (56) and the removal of TnBP and Triton X-100 were performed as described by Treščec et al (59) . The removal of the S/D agents from the fecal viromes yielded approx.…”

Section: Methodsmentioning

confidence: 99%

“…It is important to note that the majority of inactivation typically occurs within the first 30-60 minutes of the solvent/detergent treatment [50]. The removal of TnBP and Triton X-100 was performed according to the method described by Treščec et al [51]. In brief, the applied volume of Amberlite XAD-7 in the column was set to 150 % of the theoretical binding capacity to ensure a sufficient removal of TnBP and Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

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Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Rasmussen

Förster

Larsen

et al. 2023

Preprint

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Fecal microbiota transplantation (FMT) from a healthy donor to recurrent C. difficile infection (CDI) patients has proven efficient in curing the disease, possibly through bacteriophage-mediated (phages) modulation of the gut microbiome landscape. Fecal virome transplantation (FVT, sterile filtrated donor feces) has also been shown efficient for treating the disease. FVT has the advantage over FMT that no bacteria are transferred, but FVT does not exclude the risk of transferring eukaryotic viruses. We aimed to develop methodologies to obtain safer FVT by removing and/or inactivating eukaryotic viruses, while maintaining an active phage community. Donor feces were used as inoculum for a chemostat-fermentation to remove eukaryotic viruses by dilution (FVT-ChP). FVT solutions underwent solvent-detergent treatment to inactivate enveloped viruses (FVT-SDT) and pyronin-Y treatment to block the replication of RNA-viruses (FVT-PyT). The efficacy of these treatments was assessed in a CDI mouse model and compared with untreated FVT (FVT-UnT), FMT, and saline-treatment as controls. Intriguingly, 8/8 mice receiving FVT-SDT did not reach the humane endpoints until planned euthanization and expressed limited symptoms of CDI. While 5/7 saline treated mice reached the humane endpoint. Compared to the saline treatment, lower C. difficile abundance (p<0.005) in the FVT-SDT-treated mice suggested that the intervention had hampered C. difficile colonization. The mice receiving FVT-ChP and FVT-UnT tended to express alleviated CDI symptoms compared to the saline control. This proof-of-concept study may constitute the initial step of developing therapeutic tools that targets a broad spectrum of gut-related diseases and thereby substituting FMT with a safer phage-mediated therapies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Rasmussen

Förster

Larsen

et al. 2023

Preprint

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“…The fecal viromes were treated according to the recommendations from the WHO for clinical use of solvent/detergent treated plasma; incubation in 1% (w/v) tri(n-butyl) phosphate (TnBP) and 1% (w/v) Triton X-100 at 30°C for 4 hours 35 . Solvent/detergent treatment was performed by following the method of Horowitz et al 36 and the removal of TnBP and Triton X-100 was performed as described by Treščec et al 100 . In brief, the applied volume of Amberlite XAD-7 in the column was set to 150% of the theoretical binding capacity to ensure a sufficient removal of TnBP and Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

“…The fecal viromes were treated by following the recommendations from the World Health Organization (WHO) for clinical use of S/D treated plasma; incubation in 1% (w/v) tri(n-butyl) phosphate (TnBP) and 1% (w/v) Triton X-100 at 30°C for 4 hours 71 . S/D treatment was performed by following the method of Horowitz et al 34 and the removal of TnBP and Triton X-100 were performed as described by Treščec et al 72 . The removal of the S/D agents from the fecal viromes yielded approx.…”

Section: Solvent/detergent Treated Fecal Virome (Fvt-sdt)mentioning

confidence: 99%

Transfer of modified fecal viromes improve blood glucose regulation and alleviates symptoms of metabolic dysfunction-associated fatty liver disease in an obesity male mouse model

Mao

Larsen

Zachariassen

et al. 2023

Preprint

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Introduction: Recent evidence suggest a link between gut microbiome dysbiosis and metabolic syndrome, including type-2-diabetes, obesity, and non-alcoholic fatty liver disease (NAFLD). Fecal microbiota transplantation (FMT) has been explored as a way to restore a healthy gut microbiome in obese patients but poses safety concerns. The wide use of FMT is limited by safety concerns about transferring the entire fecal microbiome from one individual to another. Fecal virome transplantation (FVT) is a safer alternative that transfers bacteriophages without bacterial transfer, but still carries the risk of eukaryotic virus infection. Therefore, a safer and more effective tool for modulating gut microbiome is needed. Methods: We explored the potential of implementing three alternative FVT techniques with increased safety established in a recent study (eukaryotic viruses were either eliminated or inactivated) to ameliorate symptoms associated with a diet-induced obesity mouse model. Male mice were fed with an ad libitum high-fat diet before being euthanized (23 weeks of age) and received the different FVT treatments twice with one week of interval. Body weight was measured, oral glucose tests were performed, feces were sampled frequently, and liver, fat pads, mesenteric lymph node, and blood serum were sampled at termination of study. Results: FVT treatments had no effect on weight gain or the amount of epididymal white adipose tissue. Mice given regular untreated FVT (FVT-UnT) had a significant (p < 0.05) drop in the pathological score of their liver tissue when compared to HFD-control mice. Mice treated with a chemostat propagated fecal virome (FVT-ChP, eukaryotic viruses eliminated by dilution) improved their blood glucose regulation significantly (p < 0.05) compared to HFD-control mice. Gut microbiome analysis of both the bacterial and viral component suggested that bacteriophage-mediated modulation of the gut microbiome could be a driving factor for the observed effects. Conclusions: These results may lay the ground to develop safer bacteriophage-based therapeutic tools to restore the dysbiotic gut microbiome associated with metabolic syndrome.

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“…Non-ionic and zwitterionic detergents are compatible with IEC. Some recent works have been published on the use of IEC to separate detergents from the proteins of interest [57,58].…”

Section: Principlesmentioning

confidence: 99%

Identification, characterization and determination of metal-binding proteins by liquid chromatography. A review

Guntiñas

Bordin

Rodríguez

2002

Analytical and Bioanalytical Chemistry

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The use of liquid chromatography in the separation and determination of metal-binding proteins is reviewed. Advantages and drawbacks of different chromatographic techniques based on various principles: size exclusion, ion exchange (cationic and ionic), reversed phase and affinity, are presented and discussed. The topic "metal-binding proteins" is considered and presented from two different points of view. The first one regards metal speciation in biological samples (serum and blood). In metal speciation studies, the exact identity of the protein to which the metal is bound often remains unknown. The second point of view is that, frequently, the interest of analyzing metal-binding proteins is not related anymore to the metallic fraction of the protein, but to other chemical structures attached to the protein, such as carbohydrates, which indirectly determine how good the function of the protein is. In this review, special attention is paid to studies dealing with the glycosylation of transferrin, and with the glycated isoform of haemoglobin.

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Removal of detergent and solvent from solvent–detergent-treated immunoglobulins

Cited by 9 publications

References 19 publications

Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Transfer of modified fecal viromes improve blood glucose regulation and alleviates symptoms of metabolic dysfunction-associated fatty liver disease in an obesity male mouse model

Identification, characterization and determination of metal-binding proteins by liquid chromatography. A review

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