Removal of excess interfacial material from surface-modified emulsions using a microfluidic device with triangular post geometry

Lu, Li; Irwin, Rebecca M.; Coloma, Mikhail; Schertzer, Jeffrey W.; Chiarot, Paul R.

doi:10.1007/s10404-014-1521-9

Cited by 12 publications

(15 citation statements)

References 44 publications

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“…from the green stream into the yellow domain) and continue along the flow into the OL2 phase containing cholesterol. A comprehensive description of the oil separation performance of the triangular posts was discussed by Lu et al 59 Finally, the water in oil emulsions, covered with a monolayer of lipid-1 molecules (Fig. 1c), approach the second flow focusing region (Fig.…”

Section: Resultsmentioning

confidence: 99%

Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition

2019

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Section: Resultsmentioning

confidence: 99%

Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition

2019

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“…1, six triangular posts were built in the co-flow region. 49 The flow rate of the oil/lipid-2 stream was 400 μL h −1 . 1b) and the last (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Fig. 49 Mixing of DOPE and DOPC between the inner-and outer-leaflet occurs spontaneously and is necessary to maintain the stability of the vesicles. This qualitatively confirms the presence of both lipids in the bilayer membrane.…”

Section: Membrane Asymmetry Characterizationmentioning

confidence: 99%

“…To ensure material consistency inside the microchannel network, the glass slide was first spin-coated with a thin layer of PDMS (i.e. 49 It is noteworthy that the channel geometry can be changed to provide precise control over the emulsion and vesicle sizes. The PDMS replica and PDMS-coated slide were covalently bonded to create a sealed microfluidic device.…”

Section: Fabrication Of Microfluidic Devicesmentioning

confidence: 99%

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Continuous microfluidic fabrication of synthetic asymmetric vesicles

Schertzer

Chiarot

2015

Lab Chip

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We report on a novel microfluidic strategy for the continuous fabrication of monodisperse asymmetric vesicles with customized membrane composition, size, and luminal content. The microfluidic device encompasses a triangular post region and two flow-focusing regions. The major steps involved in the vesicle fabrication process include: (1) forming highly uniform water emulsions in an oil/inner-leaflet-lipid solution, (2) replacing the inner-leaflet-lipid solution with an outer-leaflet-lipid solution inside the microchannel network, (3) forming water-in-oil-in-water double emulsions, and (4) extracting excess oil/outer-leaflet-lipid solution from the double emulsions. Bilayer membrane asymmetry and unilamellarity are evaluated using a fluorescence quenching assay and a transmembrane protein insertion assay, respectively. Our approach addresses many of the deficiencies found in existing technologies for building vesicles, and yields strong membrane asymmetry. The ability to create and sustain membrane asymmetry is an important feature, as it is a characteristic of nearly all natural membranes. Over 80% of the vesicles remain stable for at least 6 weeks and the membrane asymmetry is maintained for over 30 hours. The asymmetric vesicles built using this strategy are collected off-chip and hold the potential to be used as model systems in membrane biology or as vehicles for drug delivery.

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“…37,53 This strategy uses a customized PDMS microfluidic device on which monodisperse symmetric or asymmetric vesicles with tailored membrane composition, size, and luminal content are built at high-throughput. The microchannels of the microfluidic device consist of a triangular post region and two flow-focusing regions, where the following steps are performed to build the GUVs: (1) inner-leaflet assembly on w/o emulsions templates formed at the first flow-focusing region, (2) replacement of the inner-leaflet-lipid with the outer-leaflet-lipid in the oil phase using a continuous hydrodynamic flow separation technique in the triangular post region, 54,55 (3) outer-leaflet assembly on w/o/w double emulsions formed at the second flow-focusing region, and (4) extraction of the intermediate oil layer from the double emulsions. Oleic acid was used as the oil phase to carry the lipids.…”

Section: Methodsmentioning

confidence: 99%

Membrane mechanical properties of synthetic asymmetric phospholipid vesicles

Doak

Schertzer

et al. 2016

Soft Matter

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Synthetic lipid vesicles have served as important model systems to study cellular membrane biology. Research has shown that the mechanical properties of bilayer membranes significantly affects their biological behavior. The properties of a lipid bilayer are governed by lipid acyl chain length, headgroup type, and the presence of membrane proteins. However, few studies have explored how membrane architecture, in particular trans-bilayer lipid asymmetry, influences membrane mechanical properties. In this study, we investigated the effects of lipid bilayer architecture (i.e. asymmetry) on the mechanical properties of biological membranes. This was achieved using a customized micropipette aspiration system and a novel microfluidic technique previously developed by our team for building asymmetric phospholipid vesicles with tailored bilayer architecture. We found that the bending modulus and area expansion modulus of the synthetic asymmetric bilayers were up to 50% larger than the values acquired for symmetric bilayers. This was caused by the dissimilar lipid distribution in each leaflet of the bilayer for the asymmetric membrane. To the best of our knowledge, this is the first report on the impact of trans-bilayer asymmetry on the area expansion modulus of synthetic bilayer membranes. Since the mechanical properties of bilayer membranes play an important role in numerous cellular processes, these results have significant implications for membrane biology studies.

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Removal of excess interfacial material from surface-modified emulsions using a microfluidic device with triangular post geometry

Cited by 12 publications

References 44 publications

Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition

Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition

Continuous microfluidic fabrication of synthetic asymmetric vesicles

Membrane mechanical properties of synthetic asymmetric phospholipid vesicles

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