1983
DOI: 10.1073/pnas.80.17.5320
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Removal of extraplatelet Na+ eliminates indomethacin-sensitive secretion from human platelets stimulated by epinephrine, ADP, and thrombin.

Abstract: We have previously observed that removal of extraplatelet Na+ markedly diminishes human platelet aggregation and secretion in response to epinephrine. The present studies demonstrate that this effect of the removal of extraplatelet Na' on platelet function is not unique to activation of platelets by 2-adrenergic agents but represents a phenomenon also evident for other platelet stimuli. Thus, platelet aggregation and secretion in response to maximal concentrations of ADP and lower concentrations of thrombin (<… Show more

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Cited by 43 publications
(14 citation statements)
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“…Thus, it is inferred that sodium deprivation may prevent activation of the cyclooxygenase. 128 Low concentrations of thrombin also increase the ^Na content of the platelets about threefold, accompanied by an increase in the fluorescence of 3,3'-dipropylthiodicarbocyanine, which represents depolarization of the platelet membrane. The extent of the depolarization depends on [Na + ] 0 , with 20% effect remaining at 29 mM Na + .…”
Section: Role Of Sodium and Phmentioning
confidence: 99%
“…Thus, it is inferred that sodium deprivation may prevent activation of the cyclooxygenase. 128 Low concentrations of thrombin also increase the ^Na content of the platelets about threefold, accompanied by an increase in the fluorescence of 3,3'-dipropylthiodicarbocyanine, which represents depolarization of the platelet membrane. The extent of the depolarization depends on [Na + ] 0 , with 20% effect remaining at 29 mM Na + .…”
Section: Role Of Sodium and Phmentioning
confidence: 99%
“…Confluent cells were washed twice with phosphate-buffered saline (PBS) containing 137 mmol/l NaCl, 2.6 mmol/l KCl, 10 mmol/l Na 2 HPO 4 , 1.8 mmol/l KH 2 PO 4 and harvested with a rubber policeman. Cells were pelleted and resuspended in lysis buffer (5 mmol/l Tris-HCl, pH 7.5, 5 mmol/l EDTA, 5 mmol/l EGTA, 0.1 mmol/l phenylmethylsulfonyl fluoride), and then homogenized in a Dounce homogenizer.…”
Section: Cell Culture and Membrane Preparationmentioning
confidence: 99%
“…This action, thought to reflect the modulatory influence of intracellular Na + on receptor signaling [4,5], is associated with rightward shifts of agonist binding curves, indicative of reduced formation of the ternary complex between the ligand-occupied receptor and G-protein [6,7]. Thus, the binding of Na + to these receptors increases R state prevalence, corresponding to a lower value of the equilibrium constant J in the extended ternary model shown in figure 1, as previously discussed by Costa et al [8].…”
mentioning
confidence: 99%
“…The role of the Na + /H + exchanger-1 (NHE-1) in platelet activation and aggregation previously has been studied using the clinically employed diuretic amiloride or its derivatives [2, 9, 17, 2123]. We previously reported the characterization of benzamide-N-(aminoiminomethyl)-4-[4-(2-furanylcarbonyl)-1-piperazinyl]-3-(methylsulfonyl) methanesulfonate (BIIB 513) and 2-methyl-5-methylsulfonyl-1-(1-pyrrollyl)-benzoyl-guanidine (EMD 85131), both specific and selective benzoylguanidine inhibitors of the NHE isoform 1 (NHE-1), compounds that confer marked cardioprotection in vivo, as measured by a reduction in arrhythmias and myocardial infarct size [38].…”
Section: Introductionmentioning
confidence: 99%