Removal of Leukocytes from Whole Blood and Erythrocyte Suspensions by Filtration through Cotton Wool

Abstract

Abstract. Using the filtration method described earlier, leukocytes were removed from units of fresh whole blood and erythrocyte suspensions (hematocrit 50%). After filtration, the units were stored for one or two weeks. Units of whole blood and erythrocyte concentrates were stored for periods up to three weeks, the leukocytes were then removed by filtration, and the units were stored for another week. During the storage period, the following parameters were measured: pH and concentrations of ATP + ADP, 2,3‐diphosphoglycerate, hemoglobin and potassium (Hb and K+ in the cell‐free supernatant). The parameters used in the present study indicate that removal of leukocytes by filtration did not cause significant alteration to stored whole blood and erythrocyte concentrates, compared to unfiltered blood stored for the same time.

The Enigma of Good Kidney-Graft Survival in the Face of Poor HLA Matches

“…The precise way in which the blood was to be prepared was not specified. Most centers gave "washed" leukocyte-poor blood, but a few used cotton-wool-filtered blood, which for all pracücal purposes IS leukocyte-free (Diepenhorst et al, 1972) As IS shown in Fig. 3, this prospective study confirmed the retrospective study with respect to the graft-protecting effect of one transfusion of leukocyte-poor blood.…”

A mathematical model for the leukocyte filtration process

“…It was found that most of the phenomena observed during leukocyte filtration experiments could be explained by a general mathematical formulation of the process [Eqs. (8) and ( superficial filtration velocity (cm . s- ') filter porosity in Diepenhorst model, Eq.…”

“…(8) and (9)], at least three filtration parameters, c, urnax and A, , should be solved independently. Since theoretical relations for these parameters in terms of the properties of the filter are not yet well developed, this can only be achieved by trial and error.…”

Blood Transfusion as Regulator of the Immune Response

“…To further reduce the incidence of especially HLA (human leukocyte antigen) alloimmunization in poly-transfused and/or transplant patients, filters were developed to selectively reduce the leukocytes in the blood (table 1). This lead to a reduction in the formation of α-HLA antibodies in these frequently transfused patients and further reduced the number of febrile complications [10,11]. When analyzing the results from different studies across the world, it is important to realize that, although whole blood is not widely used anymore, all other types of erythrocyte products are considered 'the' standard erythrocyte product in some countries.…”