Removal of Methyl Parathion from Artificial Off-Gas Using a Bioreactor Containing a Constructed Microbial Consortium

Lin, Li; Yang, Chao; Lan, Wensheng; Xie, Shouqi; Qiao, Chuanling; Liu, Junxin

doi:10.1021/es702631x

Cited by 26 publications

(12 citation statements)

References 36 publications

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“…Recently, engineered E. coli strains expressing OPH or MPH have been tested for the ability to degrade different organophosphorus pesticides (16,24,36). In 2008, a bioreactor inoculated with MPH-expressing E. coli was developed and used successfully for removal of methyl parathion from artificial off-gas (21). We are currently investigating the degradation of a wide variety of pesticides in a bioreactor with recombinant E. coli cells coexpressing OPH and MPH.…”

Section: Resultsmentioning

confidence: 99%

Cotranslocation of Methyl Parathion Hydrolase to the Periplasm and of Organophosphorus Hydrolase to the Cell Surface of Escherichia coli by the Tat Pathway and Ice Nucleation Protein Display System

Yang

Freudl

Qiao

et al. 2010

Appl Environ Microbiol

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A genetically engineered Escherichia coli strain coexpressing organophosphorus hydrolase (OPH) and methyl parathion hydrolase (MPH) was constructed for the first time by cotransforming two compatible plasmids. Since these two enzymes have different substrate specificities, the coexpression strain showed a broader substrate range than strains expressing either one of the hydrolases. To reduce the mass transport limitation of organophosphates (OPs) across the cell membrane, MPH and OPH were simultaneously translocated to the periplasm and cell surface of E. coli, respectively, by employing the twin-arginine translocation (Tat) pathway and ice nucleation protein (INP) display system. The resulting recombinant strain showed sixfold-higher whole-cell activity than the control strain expressing cytosolic OP hydrolases. The correct localization of MPH and OPH was demonstrated by cell fractionation, immunoblotting, and enzyme activity assays. No growth inhibition was observed for the recombinant E. coli strain, and suspended cultures retained almost 100% of the activity over a period of 2 weeks. Owing to its high level of activity and superior stability, the recombinant E. coli strain could be employed as a whole-cell biocatalyst for detoxification of OPs. This strategy of utilizing dual translocation pathways should open up new avenues for cotranslocating multiple functional moieties to different extracytosolic compartments of a bacterial cell.

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Section: Resultsmentioning

confidence: 99%

Cotranslocation of Methyl Parathion Hydrolase to the Periplasm and of Organophosphorus Hydrolase to the Cell Surface of Escherichia coli by the Tat Pathway and Ice Nucleation Protein Display System

Yang

Freudl

Qiao

et al. 2010

Appl Environ Microbiol

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“…Several highly toxic compounds have been biodegraded using this approach, including 4-chlorodibenzofuran (Arfmann et al 1997), parathion (Gilbert et al 2003) and methyl parathion (Li et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of hexachlorobenzene by a constructed microbial consortium

Ding

Mao

et al. 2014

World J Microbiol Biotechnol

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A consortium comprised of an engineered Escherichia coli DH5α and a natural pentachlorophenol (PCP) degrader, Sphingobium chlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA (+) camB (+) camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 μM HCB was degraded by the consortium at a rate of 0.033 nmol/mg (dry weight)/h over 24 h, accompanied by transient accumulation and immediate consumption of the intermediate PCP, detected by gas chromatography. In contrast, in the consortium comprised of Pseudomonas putida PaW340 harbouring camA (+) camB (+) camC and ATCC 39723, PCP accumulated in PaW340 cells but could not be further degraded, which may be due to a permeability barrier of Pseudomonas PaW340 for PCP transportation. The strategy of bacterial co-culture may provide an alternative approach for the bioremediation of HCB contamination.

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“…Synthetic microbial consortia have been used as an alternative to naturally occurring communities to improve and accelerate the biodegradation of pollutants 98; 99; 100; 101; 102 . For example, a co-culture of a genetically engineered Escherichia coli and a wild-type Ochrobactrum sp.…”

Section: Applications Of Synthetic Ecology In Biomedicine Metabolic mentioning

confidence: 99%

“…For example, a co-culture of a genetically engineered Escherichia coli and a wild-type Ochrobactrum sp. was established in a laboratory-scale bioreactor to degrade methyl parathion, a highly toxic pesticide commonly used for agriculture crop protection 100 . The engineered E. coli strain overproduces methyl parathion hydrolase converting methyl parathion into p-nitrophenol, which is a toxic intermediate and serves as the sole carbon, nitrogen and energy source for and degraded by Ochrobactrum sp.…”

Section: Applications Of Synthetic Ecology In Biomedicine Metabolic mentioning

confidence: 99%

Synthetic Ecology of Microbes: Mathematical Models and Applications

Zomorrodi

Segrè

2016

Journal of Molecular Biology

197

165

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As the indispensable role of natural microbial communities in many aspects of life on Earth is uncovered, the bottom-up engineering of synthetic microbial consortia with novel functions is becoming an attractive alternative to engineering single-species systems. Here, we summarize recent work on synthetic microbial communities with a particular emphasis on open challenges and opportunities in environmental sustainability and human health. We next provide a critical overview of mathematical approaches, ranging from phenomenological to mechanistic, which can be used to decipher the principles that govern the function, dynamics and evolution of microbial ecosystems. Finally, we present our outlook on key aspects of microbial ecosystems and synthetic ecology that require further developments, including the need for more efficient algorithms and a better integration of empirical methods and model-driven analysis, the importance of improving gene function annotation, and the value of a standardized library of well-characterized organisms to be used as building blocks of synthetic communities.

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Removal of Methyl Parathion from Artificial Off-Gas Using a Bioreactor Containing a Constructed Microbial Consortium

Cited by 26 publications

References 36 publications

Cotranslocation of Methyl Parathion Hydrolase to the Periplasm and of Organophosphorus Hydrolase to the Cell Surface of Escherichia coli by the Tat Pathway and Ice Nucleation Protein Display System

Cotranslocation of Methyl Parathion Hydrolase to the Periplasm and of Organophosphorus Hydrolase to the Cell Surface of Escherichia coli by the Tat Pathway and Ice Nucleation Protein Display System

Biodegradation of hexachlorobenzene by a constructed microbial consortium

Synthetic Ecology of Microbes: Mathematical Models and Applications

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