Removal of neurotoxic ethyl parathion pesticide by two-stage chemical/enzymatic treatment system using Fenton’s reagent and organophosphorous hydrolase

Abstract

Organophosphate compounds, which are essential ingredients of pesticide, plasticizer, air fuel, and chemical warfare agents, are serious neurotoxic hazardous materials. Therefore, diverse physical-chemical treatments have been attempted to degrade organophosphate compounds. In the present work, we propose a two-stage chemical and enzymatic treatment system. As a first stage, pretreatment of oxidation and coagulation using Fenton's reagent utilizing iron and hydrogen peroxide was employed. Preferentially, 1 mM ethyl parathion (EP) pesticide was largely (~80%) removed by Fenton's reagent reaction within 15 min. To remove residual EP, enzymatic treatment with organophosphorous hydrolase (OPH) from recombinant Escherichia coli was employed as a second stage. We successfully demonstrated that the proposed two-stage hybrid treatment process removed 1 mM environmental toxic EP efficiently (~98%) within 30 min.

Synthesis of Pd-M@HCS(M = Co, Ni, Cu) Bimetallic Catalysts and Their Catalytic Performance for Direct Synthesis of H2O2

“…Hydrogen peroxide (H 2 O 2 ), which has been diversely used for producing a wide range of products [1][2][3][4][5][6], is considered a green and clean raw material due to its highly atomic utilization and environmentally friendly properties. The direct synthesis of H 2 O 2 (DSHP) from hydrogen and oxygen is believed a promising method since H 2 O is the only by-product during the producing process [7][8][9].…”

Mineralization of Organophosphorous Pesticides by Electro-generated Oxidants

“…Removal of phosphorus from pesticidal effluents can be achieved by combined chemical-biological treatment wherein additives are added into the aeration chamber 11,12 . The principle behind this technique is to convert soluble phosphorus into insoluble form by complexing with metallic ions coupled with microbial phosphorus assimilation and to transfer insoluble phosphorus into sludge mass, which can be removed efficiently by any conventional solid-liquid separation device.…”