Removal of nitrate from industrial wastewaters in a pilot plant by nitrate-tolerantKlebsiella oxytoca CECT 4460 andArthrobacter globiformis CECT 4500

Piñar, Güadalupe; Oliva, José-Miguel; Sánchez-Barbero, Luis; Calvo, Vı́ctor; Ramos, Juan L.

doi:10.1002/(sici)1097-0290(19980605)58:5<510::aid-bit7>3.0.co;2-g

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…According to the literature data, the majority of microorganisms do not tolerate nitrate concentrations above 100 mM (ca 6 g NO 3 /L). However, certain strains are known (i.e., Klebsiella oxytoca CECT 4460) that tolerate a nitrate concentration of 1 M (62 g NO 3 /L) [12, 13,14].…”

Section: Nitrate Concentrationmentioning

confidence: 99%

Microbiological denitrification of red beet juice

Walkowiak-Tomczak¹

2002

European Food Research and Technology

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The purpose of study was the determination of optimal conditions for red beet juice denitrification by Paracoccus denitrificans bacteria as well as the evaluation of the usefulness of microbiologically treated juice for the production of a natural colouring preparation for foods.Total reduction of nitrates was found during bacterial culturing at 25 and 30°C, at an initial pH of 7.0-8.0 and juice osmotic pressure of 900-1100 mOsm/kg.Microbiological denitrification procedure affected colour, taste and aroma of the juice. However, the use of juice as food colouring and component was made feasible by acidification and partial evaporation of water together with volatile substances. Materials and methods MicroorganismParacoccus denitrificans strain (ATCC 19367) was used in this study. The bacteria were propagated on culture media recommend-D. Walkowiak-Tomczak ( ✉ ) ul.

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Section: Nitrate Concentrationmentioning

confidence: 99%

Microbiological denitrification of red beet juice

Walkowiak-Tomczak¹

2002

European Food Research and Technology

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“…Biological removal of nitrogenous compounds from industrial wastewaters with high nitrogen contents has been the focus of many studies, mainly because of its potential advantage and improved removal of these compounds over physical and/or chemical processes (4,9,18). Effluents from industrial facilities producing fertilizers, semiconductors, and munitions contain high nitrate loads and are difficult to treat (3,14,15,18,20,23). In fact, when these wastewaters reach conventional treatment plants a futile reduction of nitrate to nitrite takes place, which eventually leads to the full collapse of the treatment plants (10).…”

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confidence: 99%

“…To determine if the two different recombinant derivatives of K. oxytoca, K. oxytoca bearing pUPE2 and K. oxytoca GP1, exhibited higher nitrite reductase activities than the parental strain, the three strains were grown on M8 minimal medium with 40 mM nitrate and 2% (wt/vol) sucrose or 2% (wt/vol) glycerol as the sole C source (15). Cells in the mid-exponential growth phase were harvested by centrifugation, and cell extracts were prepared by sonication.…”

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confidence: 99%

Recombinant Klebsiella oxytoca Strains with Improved Efficiency in Removal of High Nitrate Loads

Piñar

Ramos

1998

Appl Environ Microbiol

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Klebsiella oxytoca CECT 4460 removes high nitrate loads from industrial wastewaters without accumulation of nitrite under optimal culture conditions; however, under nonoptimal conditions nitrite accumulates. This situation reflects an in vivo-limited functioning of nitrite reductase in this strain. As a way to overcome this limitation, an increase in the nitrite reductase gene dose inK. oxytoca CECT 4460 was considered. To achieve this, we cloned and transferred into this strain the Klebsiella pneumoniae nasB gene, which encodes assimilatory nitrite reductase (Lin et al., J. Bacteriol. 176:2551–2559, 1994). The delivery vector was either the wide-host-range plasmid pUPE2, in which the nasB gene is expressed from the Escherichia coli Plac promoter, or a mini-Tn5-Km vector, which upon random insertion in the host chromosome allowed expression of the nasB gene from an unidentified chromosomal host promoter. The effect of the increase in the dose of the nasB gene in K. oxytoca CECT 4460 on the accumulation of nitrite in the culture medium was tested in two recombinant strains. The results obtained showed that K. oxytoca CECT 4460 bearing pUPE2 accumulated 88% less nitrite than the wild-type strain, while the recombinant strain bearing the K. pneumoniae nasB gene in the host chromosome showed a 25% lower level of nitrite accumulation in the culture medium than that of the wild type.

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Genetically Engineered Microorganisms for Biodegradation of Recalcitrant Compounds

Duque

Esteve‐Núñez

Michán

et al. 2003

Encyclopedia of Environmental Microbiology

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Key Concepts Hybrid Pathways for Metabolism of Chloroorganic Compounds Modified Pathways for Alkylaromatics Hybrid Pathways for Nitroaromatics Removal of Organomercurials Improved Removal of Pollutants in the Plant Rhizosphere Tracking GEMs in Microcosms and Behavior of Recombinant Microbes

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Removal of nitrate from industrial wastewaters in a pilot plant by nitrate-tolerantKlebsiella oxytoca CECT 4460 andArthrobacter globiformis CECT 4500

Cited by 6 publications

References 18 publications

Microbiological denitrification of red beet juice

Microbiological denitrification of red beet juice

Recombinant Klebsiella oxytoca Strains with Improved Efficiency in Removal of High Nitrate Loads

Genetically Engineered Microorganisms for Biodegradation of Recalcitrant Compounds

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