Removal of Oxygen from Gas Streams

Meites, Louis; Meltes, Thelma.

doi:10.1021/ac60022a044

Cited by 266 publications

(111 citation statements)

References 3 publications

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“…The work reportedl here deals with the O., stoichiometrv anid( C(o, pro(duiction tltring ascorbic aci(I oxi(lation catalvzed by MIVrotlhcc iumiii extracts. Although some of the findings reported in this study agree with those of W;\hite ain(l Krtupka (29) (20). Standard assay reaction mixtuires coiltainedI 0.O15NM acetate buffer, 20 umoles of ascorbic acid, 10( 5M-\ EDTA; antii 0.8 ml of fuingal extract, all at pH 4.5.…”

supporting

confidence: 75%

“…Although some of the findings reported in this study agree with those of W;\hite ain(l Krtupka (29) (20). Standard assay reaction mixtuires coiltainedI 0.O15NM acetate buffer, 20 umoles of ascorbic acid, 10( 5M-\ EDTA; antii 0.8 ml of fuingal extract, all at pH 4.5. Uindler these coniditions, there was nio detectible 0., uiptake without extract or with boiled extract.…”

supporting

confidence: 75%

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Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria

Lillehoj

Smith

1966

Plant Physiol.

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Summlnlary. Ascorbic acid oxidase activity in Mllyrotheciumii verrucaria extracts resulted in°2 uptake exceeding 0.5 mole per mole of ascorbic acid and in CO2 evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10 % of the oxidized prodtuct disappeared. A comparison of the gas exchange data with the amount of ascorbic acid not accounted for indicated that the reaction couild not be explained by independent oxidase and oxygenase systems. Chromatographic examination of the reaction mixtures identified L-threonic acid. Experiments with ascorbic acid-1-14C showed that C-1 was partially decarboxylated during the oxidation. Test of the fungal extracts for enzymes that might explain the deviation from expected stoichiometry showed that phenolase, glutathione reductase, cytochrome oxidase, peroxidase and oxalic decarboxylase were not involved. Addition of azide in concentrations sufficient to block catalase increased excess 0 consumption abouit 65 %. No enzymes were found that could directly attack oxidized ascorbic acid.H,09 accumulated during oxidation in azide-blocked systems.The 02 excess could be explained by assuming the enzyme had peroxidative capacity on a reductant other than ascorbic acid. An intermediate of ascorbic acid oxidation appeared to function as the substrate yielding CO, and L-threonic acid on degradation.The increase in excess 0 ultilized in azide-blocked systems and the H20. accumulation also were explained by the proposed scheme. Another interpretation wotuld involve production of free radicals duiring ascorbic acid oxidation. Evidence for this was the ability of extracts to oxidize DPNH in the presence of ascorbic acid. Oxygen radicals formed in suich reactions were considered possible agents of degradation of ascorbic acid.Two enzymes are known that transfer electrons directly from ascorbic acid to 02. The first is the well-characterized copper oxidase from higher plants (8). The second, foulnd in a fuingus, Myrotheciutm zerrucaria (Alb. and Schw.) Dit. ex. Fr., was called

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supporting

confidence: 75%

supporting

confidence: 75%

Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria

Lillehoj

Smith

1966

Plant Physiol.

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“…2a. After O 2 was consumed by 0.2 M NaHSO 3 (Meites and Meltes, 1948), in the presence of Cu 2 þ ion, the color of DNPC remained, as shown in Fig. 2b, indicating that the reaction was an oxidation reaction and the oxidization of L-cysteine only occurred in the presence of both dissolved O 2 as an oxidant and Cu 2 þ ion as a catalyst, as demonstrated in Scheme 1.…”

Section: Resultsmentioning

confidence: 90%

“…100 mM L-cysteine in HEPES solution was incubated at 90°C for 40 min in the absence and presence of 50 nM Cu 2 þ ions, respectively, and then, HPLC-MS was employed to identify whether the L-cystine emerged. Furthermore, sodium sulfite was added into the system to confirm the function of O 2 as described by Meites and Meltes (1948). 100 mM L-cysteine with 50 nM Cu 2 þ ions in 0.2 M sodium sulfite pre-treated HEPES solution was incubated at 90°C for 40 min and then 0.72 mM CDNB was added.…”

Section: The Function Of Cu 2 þ Ion In Inhibitory Processmentioning

confidence: 99%

Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of l-cysteine

Yin

Wang

et al. 2015

Biosensors and Bioelectronics

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“…To obtain anaerobic conditions the medium was equilibrated with high purity N~ (Linde Co., Needham Heights, Massachusetts) after first bubbling the tank gas through a double train of vanadyl sulfate solution as recommended for deoxygenation by Meites and Meites (4). A vibrating platinum microelectrode (5) was used for oxygen measurements to determine the time required to obtain anaerobic conditions in the chamber when nitrogen replaced air as the gassing phase.…”

Section: Methodsmentioning

confidence: 99%

Active Sodium Transport by the Isolated Toad Bladder

Leaf

Anderson

Page

1958

The Journal of General Physiology

360

161

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Studies were made of the active ion transport by the isolated urinary bladder of the European toad, Bufo bufo, and the large American toad, Bufo marinus. The urinary bladder of the toad is a thin membrane consisting of a single layer of mucosal cells supported on a small amount of connective tissue.The bladder exhibits a characteristic transmembrane potential with the serosal surface electrically positive to the mucosal surface. Active sodium transport was demonstrated by the isolated bladder under both aerobic and anaerobic conditions. Aerobically the mean net sodium flux across the bladder wall measured with radioactive isotopes, Na ~ and Na ~, just equalled the simultaneous short-circuit current in 42 periods each of 1 hour's duration. The electrical phenomenon exhibited by the isolated membrane was thus quantitatively accounted for solely by active transport of sodium. Anaerobically the mean net sodium flux was found to be slightly less than the short-circuit current in 21 periods of observation. The Cause of this discrepancy is not known.The short-circuit current of the isolated toad bladder was regularly stimulated with pure oxytocin and vasopressin when applied to the serosal surface under aerobic and anaerobic conditions. Adrenaline failed to stimulate the short-circuit current of the toad bladder.

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Removal of Oxygen from Gas Streams

Cited by 266 publications

References 3 publications

Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria

Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria

Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of l-cysteine

Active Sodium Transport by the Isolated Toad Bladder

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