Removal of pharmaceutical’ estrogenic activity of sequencing batch reactor effluents assessed in the T47D-KBluc reporter gene assay

Data on UPLC/MS method validation for the biodegradation of pharmaceuticals and intermediates by a fungal consortium and on T47DK-Bluc reporter gene assay to assess the reduction of their estrogenic activity

“…T47D human breast cancer cells, which naturally express both human estrogen receptor (ER)-α and -β, were transferred with an estrogen-responsive element luciferase reporter gene construct [5]. The 17-β estradiol (E 2 ) agonist positive control is a natural steroid sex hormone produced by the ovaries named according to UIPAC as (8R,9S, 13S, 14S, 17S)-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol [1], with molecular mass of 272.388 g/mol. The antagonist ICI 182,780 is a synthetic steroidal antiestrogen derived from E 2 named by IUPAC as (7R, 8R, 9S, 13S, 14S, 17S)-13-methyl-7-[9-(4,4,5,5,5-pentafluoropentylsulfinyl)nonyl]-6,7,8,9,11,12,14,15, 16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol [1], with a molecular mass of 606.777 g/mol.…”

“…In the present report, authors present data on the method development and validation to confirm the removal of pharmaceutical compounds (PhCs) and their metabolites, especially the reduction of the metabolite’ estrogenic activity in a sequencing batch reactor (SBR) [1], as it has been reported that intermediate products might be more toxic than the initial parent compounds [2]. The UPLC-MS method development parameters are shown in Table 1 with the chromatogram and production spectrum in Fig.…”

“…A consortium of South African indigenous fungi Aspergillus niger , Mucor circinelloides , Trichoderma longibrachiatum , Trametes polyzona and Rhizopus microspores previously isolated and identified was found to be capable to remove the selected PhCs, namely CBZ, DCF and IBP in the SBR run at a retention time of one day. Pharmaceutical compound metabolites have exhibited higher estrogenic activity assayed in the T47D-KBluc (EEq 2.69 ± 0.17 ng/L) [1]. In the T47D-KBluc gene reporter bioassay, to determine the EEq values using Graphpad Prism software (version 4), X-values (concentrations) were log transformed and the E 2 and ICI 182,780 curves were fitted (sigmoidal function, variable slope).…”

“…In the T47D-KBluc gene reporter bioassay, to determine the EEq values using Graphpad Prism software (version 4), X-values (concentrations) were log transformed and the E 2 and ICI 182,780 curves were fitted (sigmoidal function, variable slope). Y-values and were corrected in order to correspond to dilution factors and determine their EEq values [1], [5]. The T47D-KBluc standard curves are shown in Fig.…”

Endocrine-Disrupting Compounds: An Overview on Their Occurrence in the Aquatic Environment and Human Exposure

“…Biological processes (both aerobic and anaerobic), such as activated sludge treatment [138], anaerobic digester systems [139], and fungal bioreactors [140] are also frequently used in the removal of endocrine disruptors, especially in combination with other tertiary treatments to improve their efficiency [132,141,142]. Through different enzymatic mechanisms (e.g., adsorption, accumulation and so on) bacteria, microalgae, and fungi can efficiently degrade EDCs with better performances for mixed populations with respect to individual microorganisms [143,144].…”