Removal of phytic acid and protein-phytic acid interactions in rapeseed

Effect of Phytic Acid on the In‐Vitro Rate of Digestibility of Rapeseed Protein and Amino Acids

“…Jones, Food Research Institute, Ottawa, Canada. Two low-phytate RF were prepared based on methods previously reported by Serraino and Thompson (1984). Low-phytate RF (RF-D) was prepared by dialyzing a 5% (w/v) aqueous dispersion of RF at pH 5.15 against distilled water at 4°C for 3 days followed by freeze-drying.…”

“…Its effects on mineral absorption have been well documented (Erdman and Forbes, 1977;Erdman, 1979), and have led to the development of methods aimed at removing phytic acid from vegetable protein systems (deRham and Jost, 1979;Gillberg and Tornell, 1976;Okubo et al, 1975;Serraino and Thompson, 1984;Thompson and Cho, 1984).…”

“…At alkaline pH, in the presence of cations, phytic acid forms a ternary protein-mineral-phytate complex (Cheryan, 1980). Such complex formation is believed to obstruct or inhibit the enzymatic degradation of the protein (O'Dell and De Boland, 1976;Singh and Krikorian, 1982). Others who use nitrogen solubility as a criteria for assessing digestibility (Iacobucci et al, 1973) believe that during protein hydrolysis, phytate forms peptide-phytate complexes that are insoluble, thus reducing the production of soluble nitrogen, which in turn appears to give a lower rate of hydrolysis.…”

Application of new methodology to canola protein isolation

“…These researchers found that phytic acid reductions greater than 60å were possible through solubilization but a Iarger phytic acid reduction was offset by low protein recovery. Greater success has been achieved by other researchers with the use of a phytase enzyme removing up to 884 of the phytic acid present in rapeseed (Serraino and Thompson, 1984).…”

Effects of protease enzyme supplementation on protein digestibility of legume and/or fish meal-based fish feeds

“…It forms indigestible complexes with nutrients including protein, reducing their utilisation by fish for growth (Gabriel et al, 2007;GarcõÂa-Estepa et al, 1999;Gilani et al, 2005;Hidvegi and Lasztity, 2002;Kumar et al, 2012b They are difficult to suppress cost effectively using conventional pre-treatments like heating, soaking and germination (Afify et al, 2011). Due to this use of dietary exogenous digestive enzymes especially phytase has been explored as a cheaper pre-treatment strategy of improving protein digestibility in low value plant based feeds for enhanced fish growth (Abdoulaye et al, 2011;Bedford and Partridge, 2010;Gabriel et al, 2007;Kim et al, 2006;Kumar et al, 2012a;Kumar et al, 2012b;Reddy et al, 1989;Serraino and Thompson, 1984;Wheeler and Ferrel, 1971). It has already been proven that the use of phytase in fish diets improves phosphorus absorption by fish reducing water pollution (Tudkaew et al, 2008).…”