Removal of seminal plasma prior to liquid storage of boar spermatozoa: A practice that can improve their fertilizing ability

Pavaneli, Ana Paula Pinoti; Passarelli, Marina da Silva; Freitas, Flávia Vieira de; Ravagnani, Gisele Mouro; Andrade, André Furugen Cesar de; Martins, Simone Maria Massami Kitamura; Yeste, Marc

doi:10.1016/j.theriogenology.2018.10.020

Cited by 30 publications

(20 citation statements)

References 54 publications

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“…Previous studies demonstrated a dose-and time-dependent depressing effect of boar SP on motility, which becomes increasingly apparent after 72 h of storage [3,4]. Thus, removal of SP prior to long-term liquid storage can be beneficial for maintaining sperm motility [3] and fertilising ability [5], but seems not to be applicable in AI practice. Ejaculates from different boars differ in their sperm's sensitivity to autologous SP [3].…”

Section: Introductionmentioning

confidence: 97%

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

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Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.

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Section: Introductionmentioning

confidence: 97%

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

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“…However, these roles are considered not essential for fertilization, as proved by the use of epididymal or of washed ejaculated spermatozoa for in vitro fertilization (IVF), or intra-cytoplasmatic sperm injection (ICSI) and artificial reproductive techniques (ARTs) of increasing application to alleviate the increasing infertility seen in humans, thought to be related to the detrimental impacts of the environment and certain habits on male health [ 5 , 6 , 7 ]. Under the procedures of IVF and/or sperm cryopreservation, the SP has even been considered detrimental and for decades customarily removed [ 8 , 9 ]. However, SP seems to warrant functions beyond fertilization, and is therefore related—in many species—with fertility [ 5 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Seminal Plasma: Relevant for Fertility?

Rodriguez‐Martinez

Martı́nez

Calvete

et al. 2021

IJMS

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Seminal plasma (SP), the non-cellular component of semen, is a heterogeneous composite fluid built by secretions of the testis, the epididymis and the accessory sexual glands. Its composition, despite species-specific anatomical peculiarities, consistently contains inorganic ions, specific hormones, proteins and peptides, including cytokines and enzymes, cholesterol, DNA and RNA—the latter often protected within epididymis- or prostate-derived extracellular vesicles. It is beyond question that the SP participates in diverse aspects of sperm function pre-fertilization events. The SP also interacts with the various compartments of the tubular genital tract, triggering changes in gene function that prepares for an eventual successful pregnancy; thus, it ultimately modulates fertility. Despite these concepts, it is imperative to remember that SP-free spermatozoa (epididymal or washed ejaculated) are still fertile, so this review shall focus on the differences between the in vivo roles of the SP following semen deposition in the female and those regarding additions of SP on spermatozoa handled for artificial reproduction, including cryopreservation, from artificial insemination to in vitro fertilization. This review attempts, including our own results on model animal species, to critically summarize the current knowledge of the reproductive roles played by SP components, particularly in our own species, which is increasingly affected by infertility. The ultimate goal is to reconcile the delicate balance between the SP molecular concentration and their concerted effects after temporal exposure in vivo. We aim to appraise the functions of the SP components, their relevance as diagnostic biomarkers and their value as eventual additives to refine reproductive strategies, including biotechnologies, in livestock models and humans.

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“…Moreover, while other studies have investigated whether the presence of seminal plasma during liquid storage (15–20 °C) affects pig sperm function and survival, their results seem to be less consistent. Indeed, whilst some authors reported that removal of seminal plasma prior to liquid storage is a harmful practice, as it decreases sperm motility, viability, and acrosome integrity [ 14 , 15 ], others found that its elimination might be beneficial for sperm survival and in vivo fertilizing ability [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…During capacitation, the process through which sperm cells acquire their ability to fertilize an oocyte, there are a series of changes that affect sperm motility, plasma membrane and acrosome integrity, membrane lipid disorder, mitochondrial activity, and Ca 2+ homeostasis, and involve the phosphorylation of certain proteins [ 17 , 18 , 19 , 20 ]. Although seminal plasma proteins have been reported to inhibit sperm capacitation [ 21 ], the controversial results about to which extent the presence of seminal plasma is important to maintain sperm function during liquid storage [ 16 ] make it necessary to elucidate whether this prolonged interaction can affect the ability of mammalian sperm to elicit in vitro capacitation and trigger acrosomal exocytosis. Furthermore, mounting evidence in other species, like the sheep, indicates that not only is the overall sperm motility affected during in vitro capacitation but also the proportions of motile sperm subpopulations [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Presence of Seminal Plasma during Liquid Storage of Pig Spermatozoa at 17 °C Modulates Their Ability to Elicit In Vitro Capacitation and Trigger Acrosomal Exocytosis

Pavaneli

Recuero

Chaves

et al. 2020

IJMS

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Although seminal plasma is essential to maintain sperm integrity and function, it is diluted/removed prior to liquid storage and cryopreservation in most mammalian species. This study sought to evaluate, using the pig as a model, whether storing semen in the presence of seminal plasma affects the sperm ability to elicit in vitro capacitation and acrosomal exocytosis. Upon collection, seminal plasma was separated from sperm samples, which were diluted in a commercial extender, added with seminal plasma (15% or 30%), and stored at 17 °C for 48 or 72 h. Sperm cells were subsequently exposed to capacitating medium for 4 h, and then added with progesterone to induce acrosomal exocytosis. Sperm motility, acrosome integrity, membrane lipid disorder, intracellular Ca2+ levels, mitochondrial activity, and tyrosine phosphorylation levels of glycogen synthase kinase-3 (GSK3)α/β were determined after 0, 2, and 4 h of incubation, and after 5, 30, and 60 min of progesterone addition. Results showed that storing sperm at 17 °C with 15% or 30% seminal plasma led to reduced percentages of viable spermatozoa exhibiting an exocytosed acrosome, mitochondrial membrane potential, intracellular Ca2+ levels stained by Fluo3, and tyrosine phosphorylation levels of GSK3α/β after in vitro capacitation and progesterone-induced acrosomal exocytosis. Therefore, the direct contact between spermatozoa and seminal plasma during liquid storage at 17 °C modulated their ability to elicit in vitro capacitation and undergo acrosomal exocytosis, via signal transduction pathways involving Ca2+ and Tyr phosphorylation of GSK3α/β. Further research is required to address whether such a modulating effect has any impact upon sperm fertilizing ability.

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Removal of seminal plasma prior to liquid storage of boar spermatozoa: A practice that can improve their fertilizing ability

Cited by 30 publications

References 54 publications

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Seminal Plasma: Relevant for Fertility?

The Presence of Seminal Plasma during Liquid Storage of Pig Spermatozoa at 17 °C Modulates Their Ability to Elicit In Vitro Capacitation and Trigger Acrosomal Exocytosis

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