The manuscript reports facile one step synthesis of CuO semiconductor nanorods by sol-gel aaproach for photocatalytic and bioremediation applications. Spectroscopic characterization along with X-ray diffractometry and electron microscopy studies confirmed the formation of nanorods with 12 to 14 nm diameter and 50-100 nm length. As synthesized nanorods were subjected to photocatalytic degradation of dyes viz. Methylene Orange (MO), Methylene Blue (MB), Eriochrome Black T (ET) and Congo Red (CR) in a time bound study. Comparative analysis of the data depicted that time taken for degradation of equal amount of CR was more compared to the other three dyes owing to its high molecular weight and lower diffusion rate in aqueous medium. Subsequently, the antibacterial properties of the nanorods were investigated against the gram negative Escherichia coli and gram positive Bacillus bacteria. Zone of clearance was observed in disk diffusion assays, thereby confirming the antibacterial characteristics of the nanorods. These nanorods thus hold great promise as a simple, selective and a sensitive analytical platform for the effective bio-monitoring and photocatalyst for dye degradation.