Removal of white cells from red cells by transfusion through a new filter

Sirchia, G.; Wenz, Barry; Rebulla, Paolo; Parravicini, A.; Carnelli, V.; Bertolini, Francesco

doi:10.1046/j.1537-2995.1990.30190117625.x

Cited by 86 publications

(37 citation statements)

References 21 publications

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“…Leucocyte depletion decreases the incidence of adverse transfusion outcomes such as HLA alloimmunisation or febrile non-haemolytic reactions [3, 4, 5, 6]. Furthermore, pre-storage leucocyte depletion reduces supernatant levels of leucocyte enzymes [7, 8]and improves biochemical parameters of stored human red blood cells (RBCs) [1, 9, 10, 11, 12].…”

Section: Introductionmentioning

confidence: 99%

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

et al. 2000

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Background and Objectives: Leucocyte depletion improves the quality of stored blood units. We have studied its role on blood viscosity. Materials and Methods: Viscosity of CPDA-1 blood units was measured in a Couette viscometer at shear rates of 94.5 and 0.1 s^–1 prior to and following filtration with the Leukotrap^® A1 system on day 0 and after 21 days at +4°C. Results: On day 0, high but not low shear viscosity was significantly decreased. The red blood cell morphology was unaffected. On day 21, blood viscosity was increased similarly for unfiltered and filtered samples at both shear rates. The echinocytosis observed after storage correlated with the increase in viscosity. Conclusion: Leucocyte depletion is associated with a decrease in high shear viscosity. This effect is, however, completely lost after 21 days.

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Section: Introductionmentioning

confidence: 99%

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

et al. 2000

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“…The leukocyte filtration was performed bed-side, and the efficacy of bed-side filtration has been tested by different study groups [19]. There is now general agreement that prestorage filtration will give more standardized products, because the filtration will be carried out under standardized circumstances [24, 25].…”

Section: Discussionmentioning

confidence: 99%

“…The filtration was performed at the bed-side using PALL RC100 (Meda, Copenhagen, Denmark) for erythrocyte transfusion and PALL PL50 if platelets had to be transfused. The efficacy of the PALL RC100 filter has previously been investigated by Sirchia et al [19], which found < 20 × 10 6 leukocytes per litre after filtration. This is equivalent to a leukocyte reduction of 99.9%.…”

Section: Methodsmentioning

confidence: 99%

Leukocyte Filtration Does Not Affect Lymphocyte Subpopulations and NK Cell Function in Recipients of Blood Transfusions

Mathiesen¹,

Lund²,

Brodthagen³

et al. 1998

Vox Sanguinis

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Background and objectives: The possible immunosuppressive action of blood transfusion has aroused great interest recently, particularly with respect to its effects on tumor growth and recurrence rate of malignant disease. Materials and methods: The effect of blood transfusion on lymphocyte subpopulations and NK cell function preoperatively and 6 months postoperatively was studied in 129 patients treated with elective surgery for colorectal malignancy. Forty-two patients (33%) received blood transfusions, 21 of them randomly allocated to receive leukocyte-depleted blood products. Investigation was by means of conventional laboratory methods. Results: In 21 patients receiving a median of 3 units of non-leukocyte-depleted blood products (NLD), a significant reduction in CD4+ lymphocytes (44% vs. 40%, p < 0.01) occurred. In contrast, no significant changes in CD4+ lymphocytes were observed in the 21 patients transfused with leukocyte-depleted blood products (LD). However, with respect to lymphocyte subpopulations and NK cell function, differences between the NLD and LD groups were not significant. There was a marginal decrease in HLA-DR+ lymphocytes in the NLD patients without a history of previous transfusion. Conclusions: There seems to be no major change in lymphocyte subpopulations and NK cell function 6 months after blood transfusion. Thus we cannot confirm our previous findings of a reduced number of CD20+ cells after blood transfusion.

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“…This concentration of LMWH allows the generation of thrombin when blood is exposed to a denuded vascular segment. [19][20][21][22] Blood platelets and leukocytes were reduced by a filtration procedure, 23 using an RC100 filter (PALL Corp, Glen Cove, NY). The platelet number was adjusted to 30,000 platelets/µL, mixing thrombocytopenic blood and whole blood.…”

Section: Blood Collection and Preparation Of Thrombocytopenic Bloodmentioning

confidence: 99%

Liposomes bearing fibrinogen could potentially interfere with platelet interaction and procoagulant activity

Hernández

Urbán²,

Casals³

et al. 2012

IJN

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Background:The contribution of fibrinogen (FBN) to hemostasis acting on platelet aggregation and clot formation is well established. It has been suggested that FBN-coated liposomes could be useful in restoring hemostasis. In the present study, we evaluated the modifications induced by multilamellar raw liposomes (MLV) or fibrinogen-coated liposomes (MLV-FBN) on hemostatic parameters. Materials and methods: Different experimental settings using whole blood or thrombocytopenic blood were used. Thromboelastometry, aggregation studies, platelet function analyzer (PFA-100 ® ) tests and studies under flow conditions were applied to detect the effect of MLV-FBN on hemostatic parameters. Results: The presence of MLV-FBN in whole blood modified its viscoelastic properties, prolonging clot formation time (CFT) (226.5 ± 26.1 mm versus 124.1 ± 9.4 mm; P , 0.01) but reducing clot firmness (45.4 ± 1.8 mm versus 35.5 ± 2.3 mm; P , 0.05). Under thrombocytopenic conditions, FIBTEM analysis revealed that MLV-FBN shortened clotting time (CT) compared to MLV (153.3 ± 2.8 s versus 128.0 ± 4.6 s; P , 0.05). Addition of either liposome decreased fibrin formation on the subendothelium (MLV 8.1% ± 4.7% and MLV-FBN 0.8% ± 0.5% versus control 36.4% ± 6.7%; P , 0.01), whereas only MLV-FBN significantly reduced fibrin deposition in thrombocytopenic blood (14.4% ± 6.3% versus control 34.5% ± 5.2%; P , 0.05). MLV-FBN inhibited aggregation induced by arachidonic acid (52.1% ± 8.1% versus 88.0% ± 2.1% in control; P , 0.01) and ristocetin (40.3% ± 8.8% versus 94.3% ± 1.1%; P , 0.005), but it did not modify closure times in PFA-100 ® studies. In perfusion experiments using whole blood, MLV and MLV-FBN decreased the covered surface (13.25% ± 2.4% and 9.85% ± 2.41%, respectively, versus control 22.0% ± 2.0%; P , 0.01) and the percentage of large aggregates (8.4% ± 2.3% and 3.3% ± 1.01%, respectively, versus control 14.6% ± 1.8%; P , 0.01). Conclusion:Our results reveal that, in addition to the main contribution of fibrinogen to hemostasis, MLV-FBN inhibits platelet-mediated hemostasis and coagulation mechanisms.

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Removal of white cells from red cells by transfusion through a new filter

Cited by 86 publications

References 21 publications

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

Leukocyte Filtration Does Not Affect Lymphocyte Subpopulations and NK Cell Function in Recipients of Blood Transfusions

Liposomes bearing fibrinogen could potentially interfere with platelet interaction and procoagulant activity

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