Removing Oxygen-Derived Free Radicals Stimulates Healing of Ethanol-Induced Erosive Gastritis in the Rat

Abstract

The effect of the radical removing agents, allopurinol and dimethyl sulphoxide (DMSO), on the healing rate of ethanol (1 ml of 40% solution) induced gastric mucosal injury was studied in the rat. One millilitre of 1 2 or 5 % allopurinol or DMSO were instilled into the stomach 1 24 and 48 h after giving ethanol by gavage. One hour after administration of ethanol, gastric mucosal injury was produced in all animals (20.4 ± 1.2 mm², mean ± SEM, n = 10). Treatment for 24 h with 2% allopurinol or DMSO significantly (p < 0.01) reduced the extent of the ethanol injury (11.1 ± 0.8 and 11.9 ± 0.9 mm², respectively, vs. 19.2 ± 1.1 mm2, n= 10) and this was similarly achieved by the 5% solutions (10.4 ± 0.9 and 10.2 ± 0.8 mm², respectively, vs. 19.2 ± 1.1 mm², n= 10). After treatment for 48 h, 30%of animals having 1 % allopurinol or DMSO remained with injury significantly (p < 0.001) less than that seen with ethanol alone (4.1 ± 0.4 and 3.9 ± 0.5 mm², respectively, vs. 12.1 ± 0.9 mm², n = 10); however, none treated with 2 or 5% solutions remained with any injury. Healing of this injury was confirmed microscopically and was achieved by regeneration. Thus, removing oxygen-derived free radicals stimulates the healing of ethanol-induced acute gastric mucosal injury in the rat.

Antiplasmodial and antiulcer activities of Melanthera scadens

“…This may be due to cytoprotective effect of the extract via antioxidant effects. Ethanol is also reported to cause gastric mucosal damage by stimulating the formation of leukotriene C 4 (LTC 4 ) [18] . The gastroprotective effect of the extract may in part be due to the suppression, by the extract of lipoxygenase activity.…”

“…Swiss albino mice (17)(18)(19)(20)(21)(22)(23)(24) g) and rats (120-145 g) of both sexes were used for these experiments. They were obtained from University of Uyo Animal House.…”

Anti-ulcerogenic activity of virgin coconut oil contribute to the stomach health of humankind

“…On the other hand, it has been found that oxygenderived free radicals are involved in the mechanism of acute and chronic ulceration in the gastric mucosa (Pihan et al, 1987). Furthermore, disturbances in gastric secretion, damage to the gastric mucosa, alterations in permeability, gastric mucus depletion and free radical production are observed after the administration of ethanol (Salim, 1990). These data suggest that antioxidant compounds could be active in this experimental model, producing antiulcerogenic effects.…”

Gastroprotective Effects of DAS-77 (a Phytomedicine) in Ulcer Models in Rats

“…The effects of the phytomedicine were generally comparable to those of misoprostol (50 μg/kg) and cimetidine (100 mg/kg) except in ethanol and indomethacin models in which the positive controls were superior. Incidence of ethanol-induced ulcer is predominant in the glandular part of the stomach and it has been associated with the formation of leukotriene C 4 (LTC 4 ), mast cell secretory products and reactive oxygen species [17]. Hence, the effect of DAS-77 may be mediated by the inhibition of the generation of these principles.…”