Renal Acid–Base Regulation Via Ammonia Transport in Mammals

Weiner, I. David

doi:10.1007/978-1-60327-229-2_13

Epithelial Transport Physiology 2009

DOI: 10.1007/978-1-60327-229-2_13

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Renal Acid–Base Regulation Via Ammonia Transport in Mammals

I. David Weiner

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2015

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Effect of dietary protein restriction on renal ammonia metabolism

Lee

Osis

Handlogten

et al. 2015

American Journal of Physiology-Renal Physiology

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Dietary protein restriction has multiple benefits in kidney disease. Because protein intake is a major determinant of endogenous acid production, it is important that net acid excretion change in parallel during protein restriction. Ammonia is the primary component of net acid excretion, and inappropriate ammonia excretion can lead to negative nitrogen balance. Accordingly, we examined ammonia excretion in response to protein restriction and then we determined the molecular mechanism of the changes observed. Wild-type C57Bl/6 mice fed a 20% protein diet and then changed to 6% protein developed an 85% reduction in ammonia excretion within 2 days, which persisted during a 10-day study. The expression of multiple proteins involved in renal ammonia metabolism was altered, including the ammonia-generating enzymes phosphate-dependent glutaminase (PDG) and phosphoenolpyruvate carboxykinase (PEPCK) and the ammonia-metabolizing enzyme glutamine synthetase. Rhbg, an ammonia transporter, increased in expression in the inner stripe of outer medullary collecting duct intercalated cell (OMCDis-IC). However, collecting duct-specific Rhbg deletion did not alter the response to protein restriction. Rhcg deletion did not alter ammonia excretion in response to dietary protein restriction. These results indicate 1) dietary protein restriction decreases renal ammonia excretion through coordinated regulation of multiple components of ammonia metabolism; 2) increased Rhbg expression in the OMCDis-IC may indicate a biological role in addition to ammonia transport; and 3) Rhcg expression is not necessary to decrease ammonia excretion during dietary protein restriction.

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Effect of dietary protein restriction on renal ammonia metabolism

Lee

Osis

Handlogten

et al. 2015

American Journal of Physiology-Renal Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

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Renal Acid–Base Regulation Via Ammonia Transport in Mammals

Cited by 1 publication

References 99 publications

Effect of dietary protein restriction on renal ammonia metabolism

Effect of dietary protein restriction on renal ammonia metabolism

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