Renal biochemical and histopathological alterations of diabetic rats under treatment with hydro alcoholic Morus nigra extrac

Abstract

Introduction: Morus nigra fruit is known to have antioxidant effects and used to control the blood sugar level in traditional medicine. Objectives: This study was conducted to investigate the biochemical and histopathological changes in the serum and kidneys of diabetic rats treated with hydroalcoholic M. nigra extract. Materials and Methods: In this study, 60 male Wistar rats were divided into five groups of 12 each. After induction of diabetes with alloxan, the diabetic rats were treated with hydroalcoholic extract of M. nigra at different concentrations. Then, the animals were anesthetized and the serum levels of glucose, creatinine, and urea as well as kidney tissue catalase level measured. The kidney tissue was also histopathologically examined. Results: Milder glomerular damage was seen in the group treated with 800 mg/kg of the M. nigra extract compared with diabetic and positive controls, and no difference in the expansion of mesenchymal tissue into renal glomerular vessels observed between the group treated with 800 mg/kg of M. nigra extract and diabetic and positive controls. Furthermore, creatinine levels were significantly higher and urea levels significantly lower in the group treated with 800 mg/kg of M. nigra extract than healthy and positive control groups (P<0.05). Conclusion: Administration of M. nigra extract at 800 mg/kg can prevent kidney tissue damage in diabetic rats and this fruit seems to be beneficial to patients with diabetes.

Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: targeting TNF-α inflammatory pathway

“…Long‐term hyperglycaemia is known as the driving force for glycogen accumulations in the renal tubules of diabetic rats. [ 62 ] According to the present histopathological examination, there is amelioration of glycogen accumulation and fatty degeneration of renal tubules in diabetic rats treated with BMFE, which comes in agreement with the results reported by Rahimi‐Madiseh [ 24 ] who documented that hydroalcoholic extract of M. nigra fruit may prevent damage to the kidney tissue but at higher dose (800 mg/Kg), far from the doses used in the current study. In a similar study using water extract of green tea as an antioxidant, the diabetic‐treated rats showed amelioration of the histopathological changes like fatty degeneration of renal tubule relative to diabetic rats.…”

“…Black mulberry leaves, fruits and barks are used in certain areas of the world as antidiabetic remedies, and recent investigations have proved their efficacy in lowering blood glucose levels in diabetic animals and to less extent in human subjects. [ 23–25 ] Phytochemical investigations of BMFE identified two anthocyanins: cyanidin 3‐ O‐β‐ glucoside and cyanidin 3‐ O‐β‐ rutinoside as the two major constituents of BMFE and juice [ 33,38,39 ] albeit with variable concentrations based on the geographical origin and the variety used. BMFE used in this study showed the same chemical profile of anthocyanins as outlined in Figure 1 and Table .…”

“…[ 23 ] In a separate study, administration of BMFE at 800 mg/kg prevented kidney tissue damage in diabetic rats. [ 24 ] The biological benefits of BMF intake on diabetic and non‐diabetic subjects were reported by Abdalla (2006), who considered BMF an excellent food supplement for anaemic, diabetic and hypertensive patients. [ 25 ]…”

Pharmacological Properties of Morus nigra L. (Black Mulberry) as A Promising Nutraceutical Resource

“…The effects of hydroalcoholic extract of M. nigra fruits on biochemical and histopathological changes in serum and kidney tissues have been evaluated in alloxan-induced diabetic rats [ 69 ]. Milder glomerular damage and no mesenchymal tissue expansion into renal glomerular vessels were observed in the group after 8 weeks of treatment with 800 mg/kg M. nigra fruit extract compared to those in diabetic and positive control (150 mg/kg metformin) groups.…”

To Evaluate the Antidiabetic and Rejuvenating Capability of Tissues on Alloxan Induced Diabetic Rats under the Effect of Ethanolic Leaf Extract of Coriandrum sativum: A Histopathological Study

“…On the other side, histopathology study of kidney tissues were revealed degeneration in alloxan induced tissues after treatment of extract on these group, degeneration had become normal. 49,50 …”