Renal Cell Lines for Study of Nephrotoxicity in Vitro

Abstract

The kidneys are one of the organ that can be commonly damaged by a number of toxic compounds (heavy metals, xenobiotics, drugs, etc.). To characterize the mechanism of toxicity, a variety of methods have been developed. The in vitro methods belong among the mostly used. Especially, the use of cell lines seems to be the leading approach to test and to characterize the toxicity mechanisms. At present, several cell lines of animal (from rat, dog, pig) or human origin are available. A detailed evaluation must go before any selection of a suitable cell line for experiments. Therefore, the aim of this review was to describe and to evaluate the mostly used renal cell lines.

Investigation of the Associations between a Nanomaterial’s Microrheology and Toxicology

“…Due to easy cultivation, maintenance of the functional membrane transporters and cytochromes makes them the mostly used cell line for in vitro studies. 64 The MDCK cells in the submerged culture were exposed to three different concentrations of both CNPs and dendrimers for 24 h, after which the influence on the metabolic activity (WST-1) and generation of ROS was investigated. As shown in Figure 4 A, the highest concentration (1000×) of CNP shows the maximum viability as compared to the positive control and other concentrations of CNPs and dendrimers.…”

“…The epithelial kidney cells MDCK were chosen for the experiment as the model cell lines as the kidney plays an important role in renal clearance, which makes it a favorable place for accumulation and toxicity of foreign bodies including nanoparticles. Due to easy cultivation, maintenance of the functional membrane transporters and cytochromes makes them the mostly used cell line for in vitro studies . The MDCK cells in the submerged culture were exposed to three different concentrations of both CNPs and dendrimers for 24 h, after which the influence on the metabolic activity (WST-1) and generation of ROS was investigated.…”