Renal Clearance of [14C]oxalate: Comparison of Constant-Infusion with Single-Injection Techniques

Prenen, J. A. C.; Boer, P. de; Mees, E. J. Dorhout; Endeman, H.J.; Spoor, S. M.; Oei, H. Y.

doi:10.1042/cs0630047

Cited by 33 publications

(11 citation statements)

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“…Especially, our serum oxalate reference values in normal adult subjects are similar to those of the accepted reference methods (radioisotopic methods) (8), and recent chromatographic (6) or enzymatic methods (3,7,9). Especially, our serum oxalate reference values in normal adult subjects are similar to those of the accepted reference methods (radioisotopic methods) (8), and recent chromatographic (6) or enzymatic methods (3,7,9).…”

Section: Resultssupporting

confidence: 71%

“…This disease, characterized by increased urinary excretion of oxalate, leading to recurrent urolithiasis and nephrocalcinosis, is a rare autosomal recessive inborn error of glyoxylate metabolism due to a functional defect of a liver enzyme, alanine glyoxylate aminotransferase (1). Nevertheless, recent techniques and improvements in sample processing allowed us to obtain reliable analytical methods (3,6,7) providing normal range of serum oxalate less than 3 mol/L in good agreement with in vivo isotope dilution method, which is the accepted reference method (8). Through the years, many methods have been described for the determination of oxalate in serum.…”

Section: Introductionmentioning

confidence: 99%

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Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase

Gaulier

Steghens

Lardet

et al. 1997

J. Biolumin. Chemilumin.

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Today, chemiluminescence detection reactions have become popular in analytical biochemistry essentially due to their high sensitivity. A chemiluminescent synthetic system (luminol/porphyrin) was successfully used to measure serum oxalate by determination of hydrogen peroxide generated through oxalate oxidase (EC 1.2.3.4.). This new method is efficient and simple, highly sensitive and the results obtained in normal adult subjects are in good agreement with those of approved methods. This original application of such a chemiluminescent system allowed us to achieve a sensitive serum oxalate assay (detection limit of 0.2 mumol/L) characterized by a low serum volume (200 microL) required for analysis.

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase

Gaulier

Steghens

Lardet

et al. 1997

J. Biolumin. Chemilumin.

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“…In contrast to the positive correlation found by others, between the urinary clearances of creatinine and oxalate, and a constant ratio of 2 between the clearances of oxalate arid creatinine, in individuals with normal or impaired kidney function (15,28), we were unable to identify any such relationship. However, the experimental protocols applied by those authors were largely different from ours, apart from their use of the [ 34 C]oxalate isotope dilution method.…”

Section: Discussioncontrasting

confidence: 53%

Oxalate Measurement in the Picomol Range by Ion Chromatography: Values in Fasting Plasma and Urine of Controls and Patients with Idiopathic Calcium Urolithiasis

Schwille¹,

Manoharan²,

Rümenapf³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:Oxalate was measured by ion chromatography in the ultrafiltrate of heparinized plasma from peripheral venous blood, using a membrane with a cut-off molecular weight (M r ). The following criteria were established: sensitivity 0.7 μπιοί -1~J; intra-and inter-assay coefficients of Variation 4% and 12%, respectively; precision of duplicate determinations (expressed s Standard deviation) 0.08 μηιοί Ί" 1 ; overall recovery (oxalate added and diluted, respectively) 100.7%. These qualified the method for assessment of plasma oxalate in healthy human controls (males: n = 12) s well s patients with idiopathic renal calcium urolithiasis (males: n = 22; females: n = 16). Renal calcium urolithiasis patients were subclassified into those with normocalciuria and idiopathic hypercalciuria. In male and female controls the mean values (and r nge) of plasma oxalate were 1.98 (1.4 -2.5) and 1.78 (0.7 -2.9) μπιοί -l" 1 , respectively. In male controls Ultrafiltration (membrane cut off M r 10000) revealed that 11 -16% plasma oxalate was bound to constituents having an apparent A/ r above 10000, and that with use of membranes with smaller pore size, the ultrafilterability of oxalate decreases further. In renal calcium urolithiasis the following values were elicited (μηιοί-l" 1 )' male normocalciuria 1.78 (0.8-4.0), idiopathic hypercalciuria 1.58 (1.2-2.2); female normocalciuria 1.69 (0.8 -3.6), idiopathic hypercalciuria 1.21 (0.8 -2.1). The difference from controls is significant in idiopathic hypercalciuria (males and females). In contrast, in fasting urine of renal calcium urolithiasis the oxalate excretion rate (5-45 μηιοί per 120 min) and oxalate clearance (21-328 ml per min) resemble those in controls, whereas in renal calcium urolithiasis the fractional oxalate clearance (30-357% of creatinine clearance) tended to higher values (p < 0.01, in male idiopathic hypercalciuria versus controls).^It is suggested that 1) ion chromatography allows the reliable assessment of ultrafiltrable plasma oxalate in health and disease states, 2) in renal calcium urolithiasis this technique may help to ehicidate oxalate pathophysiology, especially the mode of renal handling of oxalate.

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“…Earlier studies suggested that there was net tubular secretion of oxalate [7]. However, recent studies in man, using newer methods to measure plasma oxalate levels -a notoriously difficult analytical challenge -have suggested fractional excretions of less than 100%, or net tubular reabsorption, in the majority of normal subjects [4,8,9].…”

Section: Renal Handling Of Oxalatementioning

confidence: 99%

Oxalate and urolithiasis

Sutton¹

1997

The Management of Lithiasis

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The importance of oxalic acid in medicine derives from the extreme insolubility of its calcium salt in water, at around 12 mg or 0.13 mM per litre, which is affected little by pH above 5, but increases as the pH falls below 5 [I]. Because calcium and oxalate require to be excreted in substantial quantities by the kidneys, averaging 4-5 mM and 0.3 mM per day respectively in the adult, urinary supersaturation with calcium oxalate is the norm. Complex and elaborate mechanisms exist to prevent the formation, growth and agglomeration of crystals in the urinary tract. processes which are believed to be precursors of stone formation.Calcium oxalate is the major constituent of over 70% of upper urinary tract stones. In calcium-containing stones, oxalate is most commonly the major anion, with varying amounts of calcium phosphate. Pure calcium oxalate stones are common, whereas predominantly calcium phosphate stones are less frequent « 10%). It has generally been assumed that the determination of the relative amounts of oxalate versus phosphate in idiopathic calcium stones is of little clinical value. Gault et al. [2], using a semiquantitative infrared analytical method [3 J, have recently addressed this issue in detail, and found that phosphate stones (oxalate: phosphate ratio < I) are commoner in females, tend to be larger than predominantly oxalate stones (oxalate: phosphate> 10), and in about 250/c of patients are associated with a defect in urinary acidification. These patients did not have complete distal renal tubular acidosis, and most did not have hypocitraturia. It is unclear whether these patients should receive different therapy from the commoner idiopathic calcium oxalate stone former [4]. Aside from these idiopathic calcium phosphate stone formers, calcium phosphate stones are usual in complete distal renal tubular acidosis (RTA), and are common (though less so than calcium oxalate) in primary hyperparathyroidism [5]. Sources of urinary oxalateIt is generally stated that in normal man, some 40% of urinary oxalate is derived from serine and glycine via glyoxylate, 40% from ascorbic acid via as yet uncertain metabolic intermediates, and 5-10% from dietary oxalate. An average (Western) 1. Talati et al. (eds) The Manar;ement of Lithiasis. 57-68

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Renal Clearance of [14C]oxalate: Comparison of Constant-Infusion with Single-Injection Techniques

Cited by 33 publications

References 1 publication

Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase

Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase

Oxalate Measurement in the Picomol Range by Ion Chromatography: Values in Fasting Plasma and Urine of Controls and Patients with Idiopathic Calcium Urolithiasis

Oxalate and urolithiasis

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