Renal Clearances of Inulin, Polyfructosan‐S and a Polyethylene Glycol (PEG 1,000) in the Rat

Berglund, Fredrik

doi:10.1111/j.1748-1716.1965.tb04173.x

Cited by 31 publications

(12 citation statements)

References 23 publications

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“…PMMA, a nontoxic, nonbiodegradable polymer, has high levels of biocompatibility, which expanded its application in the biomedical field from orthopedics to wound‐dressing manufacturing . PEG, a FDA‐approved water‐soluble polymer, has frequently been used in tissue engineering, drug delivery, and surface modification of medical devices because of its biocompatibility and nonimmunogenicity . Low molecular weight PEG is rapidly cleared from the plasma by kidney, which makes the application of this polymer quite safe …”

Section: Resultsmentioning

confidence: 99%

Development of a nanofibrous wound dressing with an antifibrogenic properties in vitro and in vivo model

Poormasjedi‐Meibod

Pakyari

Jackson

et al. 2016

J Biomedical Materials Res

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Dermal fibrosis, characterized by excessive extracellular matrix (ECM), is a pathological condition with limited effective therapeutic modalities. Lack of an antiscarring dressing further impedes the preventive measures for this condition. Here, we develop a new antiscarring dressing and investigate its potential as a slow-releasing vehicle for kynurenic acid (KynA), an antifibrotic agent. KynA was incorporated into polymethyl methacrylate (PMMA) nanofibers, containing increasing concentration of polyethylene glycol (PEG). Fibre morphology, water absorption capacity, surface hydrophilicity, in vitro drug release profile, and in vivo antifibrotic effects were investigated. Increasing concentrations of PEG (1-20%) significantly increased surface hydrophilicity, water absorption capacity, and drug release. Based on the obtained release profiles, PMMA + 10% PEG was the preferred formulation for sustained KynA release up to 120 hours. In vitro studies confirmed the preservation of KynA antifibrotic properties during electrospinning, indicated by fibroblasts proliferation suppression and ECM expression modulation. In vivo application of KynA-incorporated films significantly inhibited collagen (23.89 ± 4.79 vs. 6.99 ± 0.41, collagen-I/β-actin mRNA expression, control vs. treated) and fibronectin expression (7.18 ± 1.09 vs. 2.31 ± 0.05, fibronectin/β-actin mRNA expression, control vs. treated) and enhanced the production of an ECM-degrading enzyme (2.03 ± 0.88 vs. 11.88 ± 1.16 MMP-1/β-actin mRNA expression, control vs. treated). The fabricated KynA-incorporated films can be exploited as antifibrotic wound dressings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2334-2344, 2016.

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Section: Resultsmentioning

confidence: 99%

Development of a nanofibrous wound dressing with an antifibrogenic properties in vitro and in vivo model

Poormasjedi‐Meibod

Pakyari

Jackson

et al. 2016

J Biomedical Materials Res

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“…The situation in the rat is of interest here, for Berglund and his colleagues (Berglund, 1964(Berglund, , 1965Berglund, Engberg, Persson &Ulfendahl, 1969) found that GFR decreased by about 30% as the molecular weight of the indicator substance increased from 400 to 6000 (Table 4). The point at which the clearance of polyethylene glycol became restricted in the rat was at a molecular weight of 4000 (Berglund, 1968) compared with 6000 in the dog (Shaffer, Critchfield & Carpenter, 1948).…”

Section: Renal Implicationsmentioning

confidence: 99%

“…The point at which the clearance of polyethylene glycol became restricted in the rat was at a molecular weight of 4000 (Berglund, 1968) compared with 6000 in the dog (Shaffer, Critchfield & Carpenter, 1948). Berglund (1964Berglund ( , 1965 concluded that inulin clearance gave an underestimate of GFR in the rat and attributed this to the small size of the glomerular pores in this species.…”

Section: Renal Implicationsmentioning

confidence: 99%

Passage of inulin andp-aminohippuric acid through artificial membranes: Implications for measurement of renal function

Middleton

1975

J. Membrain Biol.

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Diffusion of inulin and p-aminohippuric acid (PAH) in combined aqueous solution through artificial membranes was measured at room temperature and atmospheric pressure. The membranes had pore diameters of 26, 50, 100, 200, 250, 350, 510 or 990 A. The diffusion of PAH was only restricted with a pore size of 26 A, but inulin diffusion was restricted at 100 A. When diffusion of both solutes was unrestricted (pore diameter greater than or equal to 200 A), PAH diffused four times faster than inulin, and in restricted situations this ratio was even greater. The results of these diffusion studies allow the major and minor molecular dimensions of the solutes to be estimated. Filtration of the two solutes was studied in slowly flowing situations and also with increased temperature and pressure. Pore sizes required for unrestricted filtration were the same as for unrestricted diffusion but the passage ratio was reduced from 4 to 2. These results suggest strongly that two conditions are necessary if the glomerular filtration rate (GFR) of inulin is to equal the true GFR: membrane pore size must be at least 200 A and passage through the membranes must be by bulk transport.

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“…Measurement of renal clearance of inulin during constant infusion is still used as the reference method for estimation of GFR. The renal clearance of the inulin analogue polyfructosan does not differ from that of inulin clearance (Mertz, 1963) and has been used in a variety of experimental situations in man and animals (Berglund, 1965;Harth, 1963;Roch-Ramel, Chomety & Peters, 1968;Zender et al, 1964). In clinical practice, however, the constant infusion clearance method is less reproduceable than the single injection technique, since the latter does not need urine collection.…”

Section: Discussionmentioning

confidence: 99%

Inulin single injection clearance. Microsample technique useful in children for determination of glomerular filtration rate

Müller-Suur

Göransson

Olsen

et al. 1983

Clinical Physiology

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The agreement between single injection inulin (polyfructosan) clearance and two reference methods for glomerular filtration rate (GFR) determination, namely standard inulin constant infusion clearance and 51-Cr-EDTA single injection clearance, was investigated. Studies were also made to see whether different results were obtained when capillary and venous blood samples were used in the single injection clearance method. The investigation was made in children from one year of age, adult patients and volunteers. For the single injection technique with inulin or 51-Cr-EDTA, five to seven blood samples were taken in a period of up to 180 min after injection. A bi-exponential plasma disappearance curve was used for calculations of single injection clearances. Good agreement was obtained between venous inulin single injection clearance on the one hand, and standard inulin clearance (correlation coefficient r = 0.86) and 51-Cr-EDTA single injection clearance on the other (r = 0.85). With use of capillary blood samples in inulin single injection clearance, similar good agreement was obtained both with venous 51-Cr-EDTA and with venous inulin single injection clearance (r = 0.85 and r = 0.89 respectively). From these results we conclude that inulin single injection clearance is a reliable method for GFR determinations. This method is recommended, especially in children, as capillary blood samples can be used.

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Renal Clearances of Inulin, Polyfructosan‐S and a Polyethylene Glycol (PEG 1,000) in the Rat

Cited by 31 publications

References 23 publications

Development of a nanofibrous wound dressing with an antifibrogenic properties in vitro and in vivo model

Development of a nanofibrous wound dressing with an antifibrogenic properties in vitro and in vivo model

Passage of inulin andp-aminohippuric acid through artificial membranes: Implications for measurement of renal function

Inulin single injection clearance. Microsample technique useful in children for determination of glomerular filtration rate

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