Renal Cortical Necrosis Induced in Rabbits by Local Perfusion Followed by Systemic Administrationof Endotoxin

Abstract

In an experimental model adapted to avoid severe systemic lesions, renal cortical necrosis was frequently induced in rabbits by repeated administration of endotoxin. The experimental model involves perfusion of the kidney in situ with endotoxin. Another dose of endotoxin was given intravenously 24–28 h before or after the perfusion. A significant effect of the perfusion or the endotoxin administered first was reflected by the pronounced unilateral morphological alterations appearing after the second administration of endotoxin. Some of the kidneys with cortical necrosis had band- or ring-shaped areas of preserved juxtamedullary tissue. Heavy infiltration of leukocytes at the borders of these areas called for commentation of the histological picture of infarcts, incorrectly described even in most modern textbooks.

Distribution of endotoxin (lipopolysaccharide) in the tissues of lipopolysaccharide-responsive and -unresponsive mice

“…Certainly, LPS-induced Shwartzman reaction involves damage to kidneys (43), and the accumulation seen here may be involved in the priming for or initial damage of kidneys. A previous report demonstrates that LPS can interact in perused kidney to prepare for generalized Shwartzman (12). In addition, significantly greater accumulation of LPS in tissues such as lungs and heart of responder mice, which are known to be damaged by injection of endotoxin (43), suggests that concentration of LPS in these tissues may be important in initiating the subsequent necrosis.…”

Nierenrindennekrose

“…Zwei Fälle von Nierenrinden nekrose bei ischämischer Enterokolitis bei Frauen werden dahingehend erklärt, dass aus der Plazenta stammende zirkulierende Toxine ein generalisiertes Sanarelli-Shwartzman-Phänomen auslösten und sowohl Nierenrindennekrose als auch ischämische Kolitis erzeugten (12,40). Immunologische Ursachen werden disku tiert (27); tierexperimentell wurden ausserdem durch Endotoxin-Infektionen oder durch Verabreichung hämolysierender Escherichia-coli-Stämme Nieren rindennekrosen erzeugt (19,32,36).…”