Renal disease profoundly alters cortical interstitial cell function

Abstract

Interstitial cells were cultured from explants of the unilaterally hydronephrotic, contralateral, and normal kidneys. Two types of cells were identified in culture, macrophages, and cells which were tentatively identified as fibroblasts. Cells grew at a significantly faster rate in hydronephrotic compared to contralateral or normal kidneys. Cells from the hydronephrotic kidney increased prostaglandin (PG)E2 production in response to bradykinin. Cells from contralateral and normal renal cortex did not increase PGE2 production in response to bradykinin. These results indicate hydronephrosis induces functional changes in interstitial cells cultured from the cortex of hydronephrotic compared to contralateral and normal kidneys. The induction of increased PGE2 synthesis and bradykinin responsiveness in hydronephrotic cortex could be related to the exaggerated prostaglandin synthesis known to occur in hydronephrotic cortex. In hydronephrosis, cortical interstitial cells elaborate increased amounts of substances such as prostaglandins which have the capacity to modulate important parameters of renal function.

Abnormal Growth and Clonal Proliferation of Fibroblasts in an Animal Model of Unilateral Ureteral Obstruction

“…Contrary to Davis et al [35], who found a high proportion of macrophages in rabbit cell culture, these cells were not discernable in passage 2 cultures from rat kidneys. Primary renal cell suspensions contain macrophages which attach to culture dishes, but these cells do not grow in cell culture whereas other cells divide.…”

Prostaglandin E2 production by dispersed canine fundic mucosal cells. Contribution of macrophages and endothelial cells as major sources.

“…However, several lines of evidence obtained in studies with uterus, intestine, and kidney indicate that stromal or lamina propria elements, rather than epithelial cells, may be major PG producing cells (40)(41)(42) and that inflammatory cells specifically may be important contributors to tissue PG production. After the induction of hydronephrosis, renal PG production is markedly increased.…”

Control of the Expression of Ecto-5’-Nucleotidase in Renal Fibroblasts in Cell Culture

“…The cells displayed typical mor phological features of cultured fibroblasts. Be fore confluency, they were identical with the nonphagocytic cells obtained in tissue culture from explants of rabbit kidneys [20] (fig. 1).…”