Renal histopathology features according to various warm ischemiatimes in porcine laparoscopic and open surgery model

Sabbagh, Robert; Chawla, Arun; Tisdale, Britton; Kwan, Kevin; Chatterjee, Suman; Kwiecień, Jacek M.; Kapoor, Anil

doi:10.5489/cuaj.558

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…In the standard PN group, the renal hilum was controlled using bulldog forceps and resection was carried out with metzenbaum scissors. The hilum was clamped for 45 minute, as per previous porcine PN models, 10,11 to recreate a common clinical approach. In the off-clamp PN group, resection was completed using the novel ALTRUS Thermal Tissue device (10 mm, ConMed, Canada), which has the advantage of sealing and resecting parenchyma simultaneously.…”

Section: Surgical Techniquementioning

confidence: 99%

A novel approach to off-clamp partial nephrectomy demonstrates significant improvements in renal injury in an experimental porcine model

Olvera‐Posada

Lin

Aboalsamh

et al. 2017

CUAJ

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Introduction: We sought to design a partial nephrectomy (PN) with contralateral total nephrectomy porcine model and assess the underlying mechanisms of ischemia reperfusion injury (IRI) after PN using a novel, clinically approved resection device. Methods: Domestic male pigs (n=9) underwent left lower pole PN, allocated to either standard (Group 1) or no ischemia PN (Group 2), followed by contralateral nephrectomy. Biochemical studies were performed at baseline, Day 2, and Day 7; after sacrifice, kidneys were processed for histological analysis. Apoptotic markers were measured by Western blot analyses. Urinary biomarkers were measured to assess acute kidney injury. Results: At Day 2 following PN, there was a significant rise in serum creatinine in Group 1 compared to Group 2 (355 vs. 136 mmol/L; p=0.008). Intra-renal tissue oxygen saturation after PN was inversely correlated with postoperative creatinine (r s -0.75; p=0.012) and the grade of acute tubular necrosis (r s -0.70; p=0.036). We observed a rise in expression of pro-apoptotic markers and pro-inflammatory markers in Group 1 following PN compared to Group 2. Histological analysis revealed higher grade of apoptosis in Group 1. Conclusions: IRI associated with standard PN has a deleterious impact on acute renal function, markers of tissue injury, and histological parameters, compared to off-clamp PN using the ALTRUS device. We identified several intraoperative and postoperative markers that may be used as predictors for functional and histological injury following PN.

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Section: Surgical Techniquementioning

confidence: 99%

A novel approach to off-clamp partial nephrectomy demonstrates significant improvements in renal injury in an experimental porcine model

Olvera‐Posada

Lin

Aboalsamh

et al. 2017

CUAJ

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Extracorporeal Cardiopulmonary Resuscitation Guided by End-Tidal Carbon Dioxide—a Porcine Model

Ölander

Vikholm

Lindblom

et al. 2022

J. of Cardiovasc. Trans. Res.

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Extracorporeal membrane cardiopulmonary resuscitation (ECPR) during cardiopulmonary resuscitation (CPR) for selected cases and end-tidal carbon dioxide (ETCO2) could be used to guide initiation of ECPR. Ventricular fibrillation was induced in 12 pigs and CPR was performed until ETCO2 fell below 10 mmHg; then, ECPR was performed. Animals were divided into group short (GShort) and group long (GLong), according to time of CPR. Carotid blood flow was higher (p = 0.02) and mean arterial blood pressure lower in GLong during CPR (p < 0.05). B-Lactate was lower and pH higher in GShort (p < 0.01). In microdialysis lactate-pyruvate ratio, glycerol and glutamate increased in both groups during CPR, but considerably in GLong (p < 0.01). No difference could be seen in histopathology of the brain or kidney post-ECPR. No apparent histological differences of tissue damage in brains or levels of S100B in plasma were detected between groups. This might suggest that ETCO2 could be used as a marker for brain injury following ECPR. Graphical abstract

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A Pilot Study of Postoperative Animal Welfare as a Guidance Tool in the Development of a Kidney Autotransplantation Model With Extended Warm Ischemia

Lohmann¹,

Eijken²,

Møldrup³

et al. 2019

Transplantation Direct

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Background.This pilot study aimed to maintain acceptable animal welfare in the development of a porcine autotransplantation model with severe and incremental renal ischemic injury, a model for usage in future intervention studies. Secondary aims were to develop and test methods to collect blood and urine without the need to restrain or use sedative and avoid transportation to optimize welfare of the pig.Methods.Kidneys from 7 female pigs were subjected to incremental durations of warm ischemia (WI) 30, 45, or 75 minutes by left renal artery and vein clamping. After static cold storage, contralateral nephrectomy was performed, and the injured graft was autotransplanted and animals observed for 14 days. Animal welfare was assessed and recorded using a structured scoring sheet before and 4 days after the kidney autotransplantation. Furthermore, blood samples were drawn daily the first week and every second day the following week using a semi-central venous catheter. An ostomy bag around the genitals was tested for urine collection. Measured glomerular filtration rate was calculated using renal clearance of chromium-51-labeled ethylenediamine tetraacetic acid on day 14.Results.None of the 7 animals died during the follow-up. The animal welfare was moderately affected when applying 75 minutes of WI (n = 2), and for that reason WI was not further increased. Pigs with lower WI had no observed welfare issues. With 75 minutes of WI peak, plasma creatinine was 1486 and 1317 µmol/L, reached on day 4. Lowest glomerular filtration rate levels were observed in the pigs with 75 minutes of WI.Conclusions.WI up to 75 minutes caused the intended severely impaired renal function without significantly compromising animal welfare. Blood and urine was collected postoperatively without sedation of the pigs or use of a metabolic cage.

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Renal histopathology features according to various warm ischemiatimes in porcine laparoscopic and open surgery model

Cited by 4 publications

References 13 publications

A novel approach to off-clamp partial nephrectomy demonstrates significant improvements in renal injury in an experimental porcine model

A novel approach to off-clamp partial nephrectomy demonstrates significant improvements in renal injury in an experimental porcine model

Extracorporeal Cardiopulmonary Resuscitation Guided by End-Tidal Carbon Dioxide—a Porcine Model

A Pilot Study of Postoperative Animal Welfare as a Guidance Tool in the Development of a Kidney Autotransplantation Model With Extended Warm Ischemia

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