Renal Ischemia/Reperfusion Injury

Abstract

Renal ischemia/reperfusion injury (IRI) is an important cause of acute renal failure. Cellular and molecular responses of the kidney to IRI are complex and not fully understood. ␤1 integrins localize to the basal surface of tubular epithelium interacting with extracellular matrix components of the basal membrane, including collagen IV. Whether preservation of tubular epithelium integrity could be a therapeutic approach for IRI was assessed. The effects of HUTS-21 mAb administration, which recognizes an activation-dependent epitope of ␤1 integrins, in a rat model of IRI were investigated. Preischemic HUTS-21 administration resulted in the preservation of renal functional and histopathologic parameters. Analyses of activated ␤1 integrins expression and focal adhesion kinase phosphorylation suggest that its deactivation after IRI was prevented by HUTS-21 treatment. Moreover, HUTS-21 impaired the inflammatory response in vivo, as indicated by inhibition of proinflammatory mediators and the absence of infiltrating cells. Ex vivo adhesion assays using reperfused kidneys revealed that HUTS-21 induced a significant increase of epithelial cell attachment to collagen IV. In conclusion, the data provide evidence that HUTS-21 has a protective effect in renal IRI, preventing tubular epithelial cell detachment by preserving activated ␤1 integrins functions.

Role of Erythropoietin Induced Stem Cell Mobilization in the Repairing of Acute Kidney Injury Following Ischemia Reperfusion in Albino Rats: A Histological Study

“…In this study, the peritubular capillary congestion and inflammatory cellular infiltration seen in the interstitium between the tubules of IRI group were in agreement with an earlier study which confirmed the occurrence of inflammatory response as a squeal of ischemia [19] . Some authors mentioned that inflammatory infiltrate is an important contributor to kidney injury as neutrophils attach to activated endothelium and accumulate particularly in peritubular capillary network of outer medulla, as early as 30 minutes after reperfusion.…”

Histological Study on Effect of Mesenchymal Stem Cell Therapy on Experimental Renal Injury Induced by Ischemia/Reperfusion in Male Albino Rat

“…The incision was closed in two layers using 4-0 silk sutures, followed by topical application of garamycin cream to reduce the risk of wound infection. Sham operation: Sham-operated animals underwent the same surgical procedure, except that the clamps were not applied (15). Preparation of bone marrow derived-mesenchymal stem cells (BM-MSCs): Rat bone marrow derived, PKH26 labeled, mesenchymal stem cells (MSCs) were purchased from Biochemistry Department, Kasr Al-Ainy Medical School.…”

Extracellular Signal-Regulated Kinase Activation during Renal Ischemia/Reperfusion Mediates Focal Adhesion Dissolution and Renal Injury

“…A general phenomenon during I/R is compromised cell adhesion. [1][2][3][4] Cell adhesion is tightly controlled by proper integrity of the actin cytoskeletal network, which requires ATP. 5,6 Likewise, I/R causes cytoskeletal disruption in various tissues.…”