Renal <scp>MR</scp> angiography and perfusion in the pig using hyperpolarized water

Lipsø, Kasper Wigh; Hansen, Esben Søvsø Szocska; Tougaard, Rasmus Stilling; Laustsen, Christoffer; Ardenkjær‐Larsen, Jan Henrik

doi:10.1002/mrm.26478

Cited by 21 publications

(15 citation statements)

References 27 publications

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“…The model-free deconvolution has previously been applied in hyperpolarized experiments, yielding accurate 13 C-MTT maps with a minor deviation originating from the relaxation decay of the tracer 29 . These results are further supported by the recent hyperpolarized water perfusion in the porcine kidney 42 , demonstrating that the accurate use of formalism can determine the perfusion of the kidney.…”

Section: Discussionsupporting

confidence: 60%

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Mikkelsen

Mariager

Nørlinger

et al. 2017

Sci Rep

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11C-acetate is a positron emission tomography (PET) tracer of oxidative metabolism, whereas hyperpolarized 13C-acetate can be used in magnetic resonance imaging (MRI) for investigating specific metabolic processes. The aims of this study were to examine if the kinetic formalism of 11C-acetate PET in the kidneys is comparable to that of 13C-acetate MRI, and to compare the dynamic metabolic information of hyperpolarized 13C-acetate MRI with that obtained with 11C-acetate PET. Rats were examined with dynamic hyperpolarized 13C-acetate MRI or 11C-acetate PET before and after intravenous injection of furosemide, a loop diuretic known to alter both the hemodynamics and oxygen consumption in the kidney. The metabolic clearance rates (MCR) were estimated and compared between the two modalities experimentally in vivo and in simulations. There was a clear dependency on the mean transit time and MCR for both 13C-acetate and 11C-acetate following furosemide administration, while no dependencies on the apparent renal perfusion were observed. This study demonstrated that hyperpolarized 13C-acetate MRI is feasible for measurements of the intrarenal energetic demand via the MCR, and that the quantitative measures are correlated with those measured by 11C-acetate PET, even though the temporal window is more than 30 times longer with 11C-acetate.

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Section: Discussionsupporting

confidence: 60%

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Mikkelsen

Mariager

Nørlinger

et al. 2017

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“…Recent studies have also reported the utilization of HP urea and HP water to image renal perfusion . For example, in a rat model of IRI, Nielsen et al showed a significant reduction in the HP 13 C, 15 N–urea distribution in the injured kidney, which could be a result of a combination of reduced blood flow and altered urea transport.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in a rat model of IRI, Nielsen et al showed a significant reduction in the HP 13 C, 15 N–urea distribution in the injured kidney, which could be a result of a combination of reduced blood flow and altered urea transport. Ardenkjaer‐Larsen and co‐workers demonstrated in swine that renal cortical perfusion can be measured using high‐contrast dynamic HP water imaging. Future studies combining multiple HP probes to simultaneously assess perfusion and metabolism are warranted to further interrogate the multiple molecular events that occur during progressive kidney injuries.…”

Section: Discussionmentioning

confidence: 99%

Hyperpolarized ¹³C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

et al. 2017

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Acute kidney injury (AKI) is a major risk factor for the development of chronic kidney disease (CKD). Persistent oxidative stress and mitochondrial dysfunction are implicated across diverse forms of AKI and in the transition to CKD. In this study, we applied hyperpolarized (HP) 13C dehydroascorbate (DHA) and 13C pyruvate MR spectroscopy to investigate the renal redox capacity and mitochondrial pyruvate dehydrogenase (PDH) activity, respectively, in a murine model of AKI at baseline, and 7 days after unilateral ischemia reperfusion injury (IRI). Compared to the contralateral sham-operated kidneys, the kidneys subjected to IRI showed a significant decrease in the HP 13C Vitamin C/(Vitamin C+DHA) ratio, consistent with a decrease in redox capacity. The kidneys subjected to IRI also showed a significant decrease in the HP 13C bicarbonate/pyruvate ratio, consistent with impaired PDH activity. The IRI kidneys showed a significantly higher HP 13C lactate/pyruvate ratio at day 7 compared to baseline, although the 13C lactate/pyruvate ratio was not significantly different between the IRI and the contralateral sham-operated kidneys at day 7. Arterial spin labeling MRI demonstrated significantly reduced perfusion in the IRI kidneys. Renal tissue analysis showed corresponding increased reactive oxygen species (ROS), and reduced PDH activity in the IRI kidneys. Our results show the feasibility of HP 13C MR for the noninvasive assessment of oxidative stress and mitochondrial PDH activity following renal ischemia reperfusion injury.

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“…The procedure was similar to a previously reported . In short, the pig was anesthetized with an initial dose of intravenous (IV) propofol 12 mg, intubated, and mechanically ventilated using a 60% O 2 ‐air mix using a commercially available Avance respirator system with a built‐in CO 2 and O 2 gas monitoring unit (GE Healthcare, Brøndby, Denmark).…”

Section: Methodsmentioning

confidence: 99%

Coil profile estimation strategies for parallel imaging with hyperpolarized ¹³C MRI

Hansen

Sánchez‐Heredia

Bøgh

et al. 2019

Magnetic Resonance in Med

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Purpose To investigate auto‐ and pre‐calibration coil profile estimation for parallel imaging reconstruction of hyperpolarized 13C MRI volumetric data. Methods Parallel imaging reconstruction was studied with 3 different approaches for coil profile estimation: auto‐calibration, phantom calibration, and theoretic calibration. Acquisition was performed with a 3D stack‐of‐spirals sequence with spectral–spatial excitation and Cartesian undersampling. Parallel imaging reconstructions were done with conjugate gradient SENSE and 3D gridding with inhomogeneity correction. The approaches were compared in simulations with different SNR, through phantom experiments, and in an in vivo pig study focused on the kidneys. All imaging was done with a rigid home‐built 12‐channel 13C receive coil at 3T. Results The phantom calibrated and theoretic approaches resulted in the best structural similarities in simulations and demonstrated higher image quality in the phantom experiments compared to the auto‐calibrated approach. In vivo mapping of pyruvate uptake and lactate conversion improved for accelerated acquisitions because of a better temporal resolution. From a practical and image quality point of view, use of theoretic coil profiles led to improved results compared to the other approaches. Conclusion The success of the theoretic coil profile estimation demonstrates a negligible effect of load on sensitivity profiles at the carbon frequency at 3T. Through theoretic or phantom calibrated parallel imaging, accelerated 3D volumes could be reconstructed with sufficient sensitivity, temporal, and spatial resolution to map the metabolism of kidneys exemplifying abdominal organs. This approach overcomes a critical step in the clinical translation of parallel imaging in hyperpolarized 13C MR.

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Renal MR angiography and perfusion in the pig using hyperpolarized water

Abstract: DNP AbstractPurpose: To study hyperpolarized water as an angiography and perfusion tracer in a large animal model. Methods

Cited by 21 publications

References 27 publications

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Hyperpolarized ¹³C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

Coil profile estimation strategies for parallel imaging with hyperpolarized ¹³C MRI

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Renal MR angiography and perfusion in the pig using hyperpolarized water

Abstract: DNP AbstractPurpose: To study hyperpolarized water as an angiography and perfusion tracer in a large animal model. Methods

Cited by 21 publications

References 27 publications

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping

Hyperpolarized 13C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

Coil profile estimation strategies for parallel imaging with hyperpolarized 13C MRI

Contact Info

Product

Resources

About

Hyperpolarized ¹³C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model

Coil profile estimation strategies for parallel imaging with hyperpolarized ¹³C MRI