Renal Sodium Transporters and Water Channels

Kim, Hye-Young

doi:10.5646/jksh.2013.19.1.17

Cited by 2 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, the AQP6 which has been known to have low water permeability, acting mainly as an anion transporter, is thought to be involved in urinary acid secretion [5, 6, 97]. Furthermore, AQP6 is co-localised with H+ ATPase, suggesting that low pH could activate the protein.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of high-salt diet on mean arterial pressure, renal epithelial sodium channels and aquaporin subunits expression levels in Spontaneously Hypertensive Rats

Ramachandran

Gholami

Lam

et al. 2019

Preprint

View full text Add to dashboard Cite

27An increase in blood pressure (BP) by a high-salt (HS) diet may involve the 28 changes in the expression of epithelium sodium channels (ENaCs) and aquaporins 29 (AQPs) in the kidney which affect the sodium-and water-handling mechanisms. In the 30 present study, spontaneously hypertensive rats (SHRs) and Wistar Kyoto (WKY) rats 31 were exposed to HS and regular-salt (RS) diets for 6 weeks and fluid intake was 32 monitored. After 6 weeks, mean arterial pressure (MAP) and plasma hormonal activity 33 of atrial natriuretic peptide (ANP), levels of angiotensin II (Ang II), aldosterone and 34 arginine vasopressin (AVP) were determined. The expression of mRNA and protein 35 levels of ENaC and AQP subunits in kidneys were quantified by real-time PCR and 36 Western blotting. High-salt diet caused higher MAP only in SHRs and higher fluid 37 intake in both strains of rats when compared with their respective controls on RS diet.38 The plasma levels of Ang II and aldosterone were low in both SHRs and WKY rats fed 39 with HS diet. Meanwhile, plasma ANP activity was high in both strains of rats on HS 40 diet; whilst the AVP showed vice versa effects. The renal expression of mRNA and 41 protein levels of α-and γ-ENaCs was lowered by HS diet in both SHRs and WKY rats.42 Although β-ENaC mRNA and protein expression levels were depressed in SHRs but 43 they were enhanced in WKY rats. On the other hand, AQP-1, 2 and 7 mRNA and 44 protein expression levels were lowered in both strains of rats fed with HS diet, while 45 that of AQP-3, 4 and 6 showed no significant changes. The suppression of mRNA and 46 protein expression levels of ENaC and AQP subunits suggests that the HS-induced 47 increase in the MAP of SHRs may not be due to the renal sodium and water retention 48 solely. 49 3 50 Introduction 51 Dietary salt (i.e. sodium chloride, NaCl) intake is the most remarkably modifiable 52 environmental risk factor that attracts many studies on hypertension (HPN). It has been 53 acknowledged as an important contributing factor of the aetiology and progression of 54 HPN [1]. Despite the abundant experimental, interventional and epidemiological 55 observations demonstrating an association between dietary salt and HPN, scepticism 56 remains as to how high salt (HS) intake can be mechanistically linked to the increase in 57 blood pressure (BP). Knowing the heterogeneity of HPN, it is likely to involve the 58 intricate integration of multiple regulatory systems and the kidneys have long been 59 implicated to play a central role in regulating BP. Defects in the kidneys sodium-and 60 water-handling mechanisms have been mooted as one of the primary causes of HPN in 61 HS intake [2]. 62The kidneys have the capacity to return altered BP to baseline level by 63 increasing or decreasing sodium and water excretion in response to elevated or reduced 64 BP [3]. This is accomplished in the kidney by the presence of renal membrane-bound 65 protein i.e. epithelial sodium channel (ENaC) that fine-tune sodium reabsorption [4] 66 and aquaporins (AQPs) that ...

show abstract

Section: Discussionmentioning

confidence: 99%

“…This is accomplished in the kidney by the presence of renal membrane-bound protein i.e. epithelial sodium channel (ENaC) that fine-tune sodium reabsorption [4] and aquaporins (AQPs) that facilitate the transport of water and in some cases, other small uncharged solutes [5, 6].…”

Section: Introductionmentioning

confidence: 99%

Effect of high-salt diet on mean arterial pressure, renal epithelial sodium channels and aquaporin subunits expression levels in Spontaneously Hypertensive Rats

Ramachandran

Gholami

Lam

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The AQP6 is known to have low water permeability, acting mainly as an anion transporter, is thought to be involved in urinary acid secretion. 13,14 Furthermore, AQP6 is co-localized with H + ATPase, suggesting that low pH could activate the protein. These indicate that AQP6 is most likely not involved in the transepithelial water transport; 72 therefore, the upregulation of its mRNA (Figure 5(E)) level as a consequence of the HS diet hugely remains unexplained.…”

Section: Discussionmentioning

confidence: 99%

“…11 This is accomplished in the kidney by the presence of renal membrane-bound protein, that is, epithelial sodium channel (ENaC), sodium-potassium ATPase, sodium-hydrogen exchanger and sodium-potassium-2 chloride co-transporter in the loop of Henle, sodium-phosphate co-transporter in the proximal tubules, and sodium chloride co-transporter in the distal tubules that fine-tune sodium reabsorption 12 and aquaporins (AQPs) that facilitate the transport of water and in some cases, other small uncharged solutes. 13,14 Furthermore, there is no other sodium transporter beyond the kidney’s cortical collecting duct (CD) except ENaC which further strengthens the importance of this transporter in BP regulation. 15…”

Section: Introductionmentioning

confidence: 97%

Effects of a high-salt diet on MAP and expression levels of renal ENaCs and aquaporins in SHR

Ramachandran,

Gholami,

Lam

et al. 2023

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

An increase in blood pressure by a high-salt (HS) diet may change the expression levels of renal epithelial sodium channels (ENaCs) and aquaporins (AQPs). Spontaneously hypertensive rats (SHRs) and Wistar Kyoto (WKY) rats were exposed to HS and regular-salt (RS) diets for 6 weeks. Mean arterial pressure (MAP) and plasma atrial natriuretic peptide (ANP), angiotensin II (Ang II), aldosterone, and arginine vasopressin (AVP) levels were determined. Expression of mRNA levels of ENaCs and AQPs were quantified by real-time PCR. The MAP was higher in SHRs on the HS diet. Plasma Ang II and aldosterone levels were low while plasma ANP level was high in both strains of rats. Renal expression of mRNA levels of α-, β-, and γ-ENaCs was lowered in SHRs on the HS diet. Meanwhile, renal AQP1, AQP2, and AQP7 mRNA expression levels were lowered in both strains of rats on the HS diet. Suppression of mRNA expression levels of ENaC and AQP subunits suggests that the high-salt-induced increase in the MAP of SHR may not be solely due to renal sodium and water retention.

show abstract

Renal Sodium Transporters and Water Channels

Cited by 2 publications

References 34 publications

Effect of high-salt diet on mean arterial pressure, renal epithelial sodium channels and aquaporin subunits expression levels in Spontaneously Hypertensive Rats

Effect of high-salt diet on mean arterial pressure, renal epithelial sodium channels and aquaporin subunits expression levels in Spontaneously Hypertensive Rats

Effects of a high-salt diet on MAP and expression levels of renal ENaCs and aquaporins in SHR

Contact Info

Product

Resources

About